In silico recombinant plasmid design of pHA171 with phdABCD insertion for ethidium bromide degradation

In silico recombinant plasmid design of pHA171 with phdABCD insertion for ethidium bromide degradation

Background: Ethidium bromide is a common reagent that is used in nucleic acid staining. However, ethidium bromide has toxic and carcinogenic properties that are harmful to the environment. Phenanthrene dioxygenase (encoded by phdA, phdB, phdC, and phdD genes) in Nocardioides sp. KP7 can oxidize the...

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Bibliographic Details
Main Authors: Muhammad Ilham Fahri, Rabiah Musfira Alatiffa, Sania Isma Yanti, Indira Prakoso, Alysha Naomi Mashitah
Format: Article
Language:English
Published: Indonesian Society for Biochemistry and Molecular Biology 2021-08-01
Series:Acta Biochimica Indonesiana
Subjects:
bioinformatic
bioremediation
Escherichia coli
ethidium bromide
phenanthridine
Online Access:https://pbbmi.org/newjurnal/index.php/actabioina/article/view/7

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