Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010
Introduction: Salmonella enterica serotype Typhimuri-um variant 5- is a pathogen closely related to animals, especially pigeons, which has been also associ- ated in rare cases with sporadic infections in humans. However, epidemiological surveillance systems have enabled the detection of this variant...
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| Format: | Article |
| Language: | Spanish |
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Universidad de Antioquia
2014-01-01
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| Series: | Iatreia |
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| Online Access: | http://www.iatreia.udea.edu.co/index.php/iatreia/article/view/14327/15420 |
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| author | Díaz Osorio, Miguel Ángel Díaz Guevara, Paula Lucía Rodríguez Cárdenas, Edna Catering Montaño Valencia, Lucy Angeline Medina Alfonso, Mabel Idaliana Realpe, María Elena González Patiño, Gloria Isabel |
| author_facet | Díaz Osorio, Miguel Ángel Díaz Guevara, Paula Lucía Rodríguez Cárdenas, Edna Catering Montaño Valencia, Lucy Angeline Medina Alfonso, Mabel Idaliana Realpe, María Elena González Patiño, Gloria Isabel |
| author_sort | Díaz Osorio, Miguel Ángel |
| collection | DOAJ |
| description | Introduction: Salmonella enterica serotype Typhimuri-um variant 5- is a pathogen closely related to animals, especially pigeons, which has been also associ- ated in rare cases with sporadic infections in humans. However, epidemiological surveillance systems have enabled the detection of this variant in human out- breaks.
Objective: To characterize by means of phenotypic and genotypic techniques the isolates of Salmonella Typhimurium variant 5- associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia (2010), in order to establish their molecular relationships.
Materials and methods: Twelve isolates of Salmonella -spp., were analyzed by biochemical, serotyping and antimicrobial susceptibility tests. Pulsed-field gel electrophoresis (PFGE) with Xball Blnl enzymes was used to establish their molecular relationships
Results: All isolates were confirmed as Salmonella spp. They were resistant to tetracycline and streptomycin and sensitive to the rest of antibiotics tested. Eleven isolates were identified as Salmonella Typhimurium variant 5- and grouped in COIN10.JPX.X01.0168 pattern using the enzyme Xball two isolates in this group were confirmed using the enzyme Blnl with the COIN10.JPX.A26.0002 pattern. One isolate was identified as Salmonella Typhimurium with COIN10.JPX.X01.0221 pattern with the enzyme Xbal
Conclusion: This is the first outbreak in Colombia of foodborne illness epidemiologically associated with isolates of Typhimurium variant 5 -, which Epidemiologic Sur were phenotypically and genetically related. |
| first_indexed | 2024-12-11T12:15:46Z |
| format | Article |
| id | doaj.art-27e7f4f0ee9e437082bffebad496d708 |
| institution | Directory Open Access Journal |
| issn | 0121-0793 2011-7965 |
| language | Spanish |
| last_indexed | 2024-12-11T12:15:46Z |
| publishDate | 2014-01-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Iatreia |
| spelling | doaj.art-27e7f4f0ee9e437082bffebad496d7082022-12-22T01:07:39ZspaUniversidad de AntioquiaIatreia0121-07932011-79652014-01-012712330Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010Díaz Osorio, Miguel Ángel 0Díaz Guevara, Paula Lucía 1Rodríguez Cárdenas, Edna Catering 2Montaño Valencia, Lucy Angeline 3Medina Alfonso, Mabel Idaliana 4Realpe, María Elena 5González Patiño, Gloria Isabel 6Instituto Nacional de SaludLaboratorio de Salud Pública de BoyacáInstituto Nacional de SaludLaboratorio de Salud Pública de BoyacáLaboratorio de Salud Pública de BoyacáLaboratorio de Salud Pública de BoyacáInstituto Nacional de SaludIntroduction: Salmonella enterica serotype Typhimuri-um variant 5- is a pathogen closely related to animals, especially pigeons, which has been also associ- ated in rare cases with sporadic infections in humans. However, epidemiological surveillance systems have enabled the detection of this variant in human out- breaks. Objective: To characterize by means of phenotypic and genotypic techniques the isolates of Salmonella Typhimurium variant 5- associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia (2010), in order to establish their molecular relationships. Materials and methods: Twelve isolates of Salmonella -spp., were analyzed by biochemical, serotyping and antimicrobial susceptibility tests. Pulsed-field gel electrophoresis (PFGE) with Xball Blnl enzymes was used to establish their molecular relationships Results: All isolates were confirmed as Salmonella spp. They were resistant to tetracycline and streptomycin and sensitive to the rest of antibiotics tested. Eleven isolates were identified as Salmonella Typhimurium variant 5- and grouped in COIN10.JPX.X01.0168 pattern using the enzyme Xball two isolates in this group were confirmed using the enzyme Blnl with the COIN10.JPX.A26.0002 pattern. One isolate was identified as Salmonella Typhimurium with COIN10.JPX.X01.0221 pattern with the enzyme Xbal Conclusion: This is the first outbreak in Colombia of foodborne illness epidemiologically associated with isolates of Typhimurium variant 5 -, which Epidemiologic Sur were phenotypically and genetically related.http://www.iatreia.udea.edu.co/index.php/iatreia/article/view/14327/15420Epidemiologic SurveillanceFoodborne DiseasesSalmonella TyphimuriumSerotypingSalmonella Infections Enfermedades Transmitidas por los AlimentosInfecciones por Salmonella Salmonella TyphimuriumSerotipificaciónVigilancia Epidemiológica |
| spellingShingle | Díaz Osorio, Miguel Ángel Díaz Guevara, Paula Lucía Rodríguez Cárdenas, Edna Catering Montaño Valencia, Lucy Angeline Medina Alfonso, Mabel Idaliana Realpe, María Elena González Patiño, Gloria Isabel Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 Iatreia Epidemiologic Surveillance Foodborne Diseases Salmonella Typhimurium Serotyping Salmonella Infections Enfermedades Transmitidas por los Alimentos Infecciones por Salmonella Salmonella Typhimurium Serotipificación Vigilancia Epidemiológica |
| title | Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 |
| title_full | Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 |
| title_fullStr | Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 |
| title_full_unstemmed | Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 |
| title_short | Phenotypic and genotypic characterization of Salmonella Typhimurium variant 5- isolates associated with an outbreak of food-borne disease in Paz de Rio, Boyacá, Colombia, in 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 = Caracterización fenotípica y genotípica de Salmonella Typhimurium variante 5- asociada a un brote de enfermedad transmitida por alimentos en el municipio de Paz de Río, Boyacá, 2010 |
| title_sort | phenotypic and genotypic characterization of salmonella typhimurium variant 5 isolates associated with an outbreak of food borne disease in paz de rio boyaca colombia in 2010 caracterizacion fenotipica y genotipica de salmonella typhimurium variante 5 asociada a un brote de enfermedad transmitida por alimentos en el municipio de paz de rio boyaca 2010 caracterizacion fenotipica y genotipica de salmonella typhimurium variante 5 asociada a un brote de enfermedad transmitida por alimentos en el municipio de paz de rio boyaca 2010 |
| topic | Epidemiologic Surveillance Foodborne Diseases Salmonella Typhimurium Serotyping Salmonella Infections Enfermedades Transmitidas por los Alimentos Infecciones por Salmonella Salmonella Typhimurium Serotipificación Vigilancia Epidemiológica |
| url | http://www.iatreia.udea.edu.co/index.php/iatreia/article/view/14327/15420 |
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