Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence
Sheath blight of rice is a destructive disease that could be calamitous to rice cultivation. The significant objective of this study is to contemplate the proteomic analysis of the high virulent and less virulent isolate of <i>Rhizoctonia solani</i> using a quantitative LC-MS/MS-based pr...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/4/370 |
| _version_ | 1797410542137114624 |
|---|---|
| author | Seenichamy Rathinam Prabhukarthikeyan Chidambaranathan Parameswaran Shraddha Bhaskar Sawant Ramasamy Naveenkumar Arabinda Mahanty Umapathy Keerthana Manoj Kumar Yadav Annamalai Anandan Periyasamy Panneerselvam Manas Kumar Bag Prakash Chandra Rath |
| author_facet | Seenichamy Rathinam Prabhukarthikeyan Chidambaranathan Parameswaran Shraddha Bhaskar Sawant Ramasamy Naveenkumar Arabinda Mahanty Umapathy Keerthana Manoj Kumar Yadav Annamalai Anandan Periyasamy Panneerselvam Manas Kumar Bag Prakash Chandra Rath |
| author_sort | Seenichamy Rathinam Prabhukarthikeyan |
| collection | DOAJ |
| description | Sheath blight of rice is a destructive disease that could be calamitous to rice cultivation. The significant objective of this study is to contemplate the proteomic analysis of the high virulent and less virulent isolate of <i>Rhizoctonia solani</i> using a quantitative LC-MS/MS-based proteomic approach to identify the differentially expressed proteins promoting higher virulence. Across several rice-growing regions in Odisha, Eastern India, 58 <i>Rhizoctonia</i> isolates were obtained. All the isolates varied in their pathogenicity. The isolate RS15 was found to be the most virulent and RS22 was identified as the least virulent. The PCR amplification confirmed that the RS15 and RS22 belonged to the <i>Rhizoctonia</i> subgroup of AG1-IA with a specific primer. The proteomic information generated has been deposited in the PRIDE database with PXD023430. The virulent isolate consisted of 48 differentially abundant proteins, out of which 27 proteins had higher abundance, while 21 proteins had lower abundance. The analyzed proteins acquired functionality in fungal development, sporulation, morphology, pathogenicity, detoxification, antifungal activity, essential metabolism and transcriptional activities, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi. A Quantitative Real-Time PCR (qRT-PCR) was used to validate changes in differentially expressed proteins at the mRNA level for selected genes. The abundances of proteins and transcripts were positively correlated. This study provides the role of the proteome in the pathogenicity of <i>R. solani</i> AG1-IA in rice and underpins the mechanism behind the pathogen’s virulence in causing sheath blight disease. |
| first_indexed | 2024-03-09T04:31:33Z |
| format | Article |
| id | doaj.art-27ec522db0e14400b9f0524dbaedea45 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:31:33Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-27ec522db0e14400b9f0524dbaedea452023-12-03T13:34:38ZengMDPI AGJournal of Fungi2309-608X2022-04-018437010.3390/jof8040370Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in VirulenceSeenichamy Rathinam Prabhukarthikeyan0Chidambaranathan Parameswaran1Shraddha Bhaskar Sawant2Ramasamy Naveenkumar3Arabinda Mahanty4Umapathy Keerthana5Manoj Kumar Yadav6Annamalai Anandan7Periyasamy Panneerselvam8Manas Kumar Bag9Prakash Chandra Rath10ICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaSheath blight of rice is a destructive disease that could be calamitous to rice cultivation. The significant objective of this study is to contemplate the proteomic analysis of the high virulent and less virulent isolate of <i>Rhizoctonia solani</i> using a quantitative LC-MS/MS-based proteomic approach to identify the differentially expressed proteins promoting higher virulence. Across several rice-growing regions in Odisha, Eastern India, 58 <i>Rhizoctonia</i> isolates were obtained. All the isolates varied in their pathogenicity. The isolate RS15 was found to be the most virulent and RS22 was identified as the least virulent. The PCR amplification confirmed that the RS15 and RS22 belonged to the <i>Rhizoctonia</i> subgroup of AG1-IA with a specific primer. The proteomic information generated has been deposited in the PRIDE database with PXD023430. The virulent isolate consisted of 48 differentially abundant proteins, out of which 27 proteins had higher abundance, while 21 proteins had lower abundance. The analyzed proteins acquired functionality in fungal development, sporulation, morphology, pathogenicity, detoxification, antifungal activity, essential metabolism and transcriptional activities, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi. A Quantitative Real-Time PCR (qRT-PCR) was used to validate changes in differentially expressed proteins at the mRNA level for selected genes. The abundances of proteins and transcripts were positively correlated. This study provides the role of the proteome in the pathogenicity of <i>R. solani</i> AG1-IA in rice and underpins the mechanism behind the pathogen’s virulence in causing sheath blight disease.https://www.mdpi.com/2309-608X/8/4/370<i>Rhizoctonia solani</i> AG1-IAsheath blightLC-MS/MSvirulent proteinsfungal proteomics |
| spellingShingle | Seenichamy Rathinam Prabhukarthikeyan Chidambaranathan Parameswaran Shraddha Bhaskar Sawant Ramasamy Naveenkumar Arabinda Mahanty Umapathy Keerthana Manoj Kumar Yadav Annamalai Anandan Periyasamy Panneerselvam Manas Kumar Bag Prakash Chandra Rath Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence Journal of Fungi <i>Rhizoctonia solani</i> AG1-IA sheath blight LC-MS/MS virulent proteins fungal proteomics |
| title | Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence |
| title_full | Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence |
| title_fullStr | Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence |
| title_full_unstemmed | Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence |
| title_short | Comparative Proteomic Analysis of <i>Rhizoctonia solani</i> Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence |
| title_sort | comparative proteomic analysis of i rhizoctonia solani i isolates identifies the differentially expressed proteins with roles in virulence |
| topic | <i>Rhizoctonia solani</i> AG1-IA sheath blight LC-MS/MS virulent proteins fungal proteomics |
| url | https://www.mdpi.com/2309-608X/8/4/370 |
| work_keys_str_mv | AT seenichamyrathinamprabhukarthikeyan comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT chidambaranathanparameswaran comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT shraddhabhaskarsawant comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT ramasamynaveenkumar comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT arabindamahanty comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT umapathykeerthana comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT manojkumaryadav comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT annamalaianandan comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT periyasamypanneerselvam comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT manaskumarbag comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence AT prakashchandrarath comparativeproteomicanalysisofirhizoctoniasolaniiisolatesidentifiesthedifferentiallyexpressedproteinswithrolesinvirulence |