Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells
<i>Papaver rhoeas</i> plant is common in many regions worldwide and contributes to the landscape with its red flower. In the present study we first carried out morphological investigation by optical and scanning electron microscopy of the ovules within the ovary. After ovules’ isolation...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1850 |
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| author | Elisa Ovidi Valentina Laghezza Masci Stefania Garzoli Gabriella Gambellini Saran Vittoria Keita Daniele Zago Giovanni Turchetti Lorenzo Modesti Antonio Tiezzi |
| author_facet | Elisa Ovidi Valentina Laghezza Masci Stefania Garzoli Gabriella Gambellini Saran Vittoria Keita Daniele Zago Giovanni Turchetti Lorenzo Modesti Antonio Tiezzi |
| author_sort | Elisa Ovidi |
| collection | DOAJ |
| description | <i>Papaver rhoeas</i> plant is common in many regions worldwide and contributes to the landscape with its red flower. In the present study we first carried out morphological investigation by optical and scanning electron microscopy of the ovules within the ovary. After ovules’ isolation we prepared extracts to test possible cytotoxic activities on HL60 leukemia human cells and investigated the extracts using thin-layer chromatography (TLC) and gas-chromatography/mass spectrometry (GC-MS). <i>P. rhoeas</i> ovules showed an elongated, round shape and the presence of ordered sculptures on the ovule surface. The ovule extracts showed cytotoxic activity on HL60 human cells mainly found in some TLC-isolated spots. Compounds consisting of active spots were identified by GC-MS investigations. Our findings on the <i>P. rhoeas</i> ovule compounds open perspectives for further investigations of TLC-isolated spots on other human cancer cell lines. |
| first_indexed | 2024-03-10T20:23:57Z |
| format | Article |
| id | doaj.art-27ecca3541ca45eeb0c2286457ce2ae2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:23:57Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-27ecca3541ca45eeb0c2286457ce2ae22023-11-19T21:54:34ZengMDPI AGMolecules1420-30492020-04-01258185010.3390/molecules25081850Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human CellsElisa Ovidi0Valentina Laghezza Masci1Stefania Garzoli2Gabriella Gambellini3Saran Vittoria Keita4Daniele Zago5Giovanni Turchetti6Lorenzo Modesti7Antonio Tiezzi8Department for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00100 Rome, ItalyCenter of Electron Microscopy, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agro-food and Forestal systems, Tuscia University, 01100 Viterbo, Italy<i>Papaver rhoeas</i> plant is common in many regions worldwide and contributes to the landscape with its red flower. In the present study we first carried out morphological investigation by optical and scanning electron microscopy of the ovules within the ovary. After ovules’ isolation we prepared extracts to test possible cytotoxic activities on HL60 leukemia human cells and investigated the extracts using thin-layer chromatography (TLC) and gas-chromatography/mass spectrometry (GC-MS). <i>P. rhoeas</i> ovules showed an elongated, round shape and the presence of ordered sculptures on the ovule surface. The ovule extracts showed cytotoxic activity on HL60 human cells mainly found in some TLC-isolated spots. Compounds consisting of active spots were identified by GC-MS investigations. Our findings on the <i>P. rhoeas</i> ovule compounds open perspectives for further investigations of TLC-isolated spots on other human cancer cell lines.https://www.mdpi.com/1420-3049/25/8/1850<i>Papaver rhoeas</i>ovulesplant compoundscytotoxic activityGC-MS |
| spellingShingle | Elisa Ovidi Valentina Laghezza Masci Stefania Garzoli Gabriella Gambellini Saran Vittoria Keita Daniele Zago Giovanni Turchetti Lorenzo Modesti Antonio Tiezzi Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells Molecules <i>Papaver rhoeas</i> ovules plant compounds cytotoxic activity GC-MS |
| title | Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells |
| title_full | Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells |
| title_fullStr | Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells |
| title_full_unstemmed | Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells |
| title_short | Antiproliferative Properties of <i>Papaver rhoeas</i> Ovule Extracts and Derived Fractions Tested on HL60 Leukemia Human Cells |
| title_sort | antiproliferative properties of i papaver rhoeas i ovule extracts and derived fractions tested on hl60 leukemia human cells |
| topic | <i>Papaver rhoeas</i> ovules plant compounds cytotoxic activity GC-MS |
| url | https://www.mdpi.com/1420-3049/25/8/1850 |
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