Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification
Heterotrophic nitrification–aerobic denitrification (HN-AD) is an efficient nitrogen removal process and the genus <i>Paracoccus</i> is one important group of the HN-AD bacteria. During an investigation of the microbial diversity in marine ranching of the Pearl River Estuary (PR China),...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1532 |
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| author | Kun Zhang Qi Zeng Rouyun Jiang Songbiao Shi Jian Yang Lijuan Long Xinpeng Tian |
| author_facet | Kun Zhang Qi Zeng Rouyun Jiang Songbiao Shi Jian Yang Lijuan Long Xinpeng Tian |
| author_sort | Kun Zhang |
| collection | DOAJ |
| description | Heterotrophic nitrification–aerobic denitrification (HN-AD) is an efficient nitrogen removal process and the genus <i>Paracoccus</i> is one important group of the HN-AD bacteria. During an investigation of the microbial diversity in marine ranching of the Pearl River Estuary (PR China), three bacterial strains, designated SCSIO 75817<sup>T</sup>, SCSIO 76264<sup>T</sup> and SCSIO 80058<sup>T</sup>, were isolated from sediments. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the three strains belonged to the genus <i>Paracoccus</i> and their closest neighbors were <i>P. isoporae</i> DSM 22220<sup>T</sup> (97.6–98.0%), <i>P</i>. <i>aurantiacus</i> CGMCC 1.13898<sup>T</sup> (97.3–97.6%) and <i>P</i>. <i>xiamenensis</i> MCCC 1A16381<sup>T</sup> (97.1–97.4%), respectively. The analysis results of 16S rRNA gene similarity, ANI, AAI and dDDH showed that the pairwise similarities between these three strains and their closest neighbors were 97.4–98.5%, 76.9–81.0%, 75.5–79.6% and 20.3–23.3%, respectively. Polyphasic taxonomic data of the phylogenetic, phenotypic and chemotaxonomic analyses indicate that these strains represent three novel species in the genus <i>Paracoccus</i>, for which the names <i>Paracoccus aerodenitrificans</i> sp. nov., <i>Paracoccus sediminicola</i> sp. nov. and <i>Paracoccus albus</i> sp. nov. are proposed, respectively. The study also demonstrated the heterotrophic nitrification–aerobic denitrification (HN-AD) ability of the novel species <i>P. aerodenitrificans</i> SCSIO 75817<sup>T</sup>. When it was aerobically cultivated at 28 °C using NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N and NO<sub>2</sub><sup>−</sup>-N as the sole nitrogen sources, the nitrogen removal efficiencies were 73.4, 55.27 and 49.2%, respectively, and the maximum removal rates were 3.05, 1.82 and 1.63 mg/L/h, respectively. The results suggest that it has promising potential for wastewater treatment. |
| first_indexed | 2024-03-11T02:08:02Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-11T02:08:02Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-27edc5f3bb0141d6b17a7395510f14222023-11-18T11:43:12ZengMDPI AGMicroorganisms2076-26072023-06-01116153210.3390/microorganisms11061532Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic DenitrificationKun Zhang0Qi Zeng1Rouyun Jiang2Songbiao Shi3Jian Yang4Lijuan Long5Xinpeng Tian6CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Sanya Institute of Oceanology, SCSIO, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaHeterotrophic nitrification–aerobic denitrification (HN-AD) is an efficient nitrogen removal process and the genus <i>Paracoccus</i> is one important group of the HN-AD bacteria. During an investigation of the microbial diversity in marine ranching of the Pearl River Estuary (PR China), three bacterial strains, designated SCSIO 75817<sup>T</sup>, SCSIO 76264<sup>T</sup> and SCSIO 80058<sup>T</sup>, were isolated from sediments. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the three strains belonged to the genus <i>Paracoccus</i> and their closest neighbors were <i>P. isoporae</i> DSM 22220<sup>T</sup> (97.6–98.0%), <i>P</i>. <i>aurantiacus</i> CGMCC 1.13898<sup>T</sup> (97.3–97.6%) and <i>P</i>. <i>xiamenensis</i> MCCC 1A16381<sup>T</sup> (97.1–97.4%), respectively. The analysis results of 16S rRNA gene similarity, ANI, AAI and dDDH showed that the pairwise similarities between these three strains and their closest neighbors were 97.4–98.5%, 76.9–81.0%, 75.5–79.6% and 20.3–23.3%, respectively. Polyphasic taxonomic data of the phylogenetic, phenotypic and chemotaxonomic analyses indicate that these strains represent three novel species in the genus <i>Paracoccus</i>, for which the names <i>Paracoccus aerodenitrificans</i> sp. nov., <i>Paracoccus sediminicola</i> sp. nov. and <i>Paracoccus albus</i> sp. nov. are proposed, respectively. The study also demonstrated the heterotrophic nitrification–aerobic denitrification (HN-AD) ability of the novel species <i>P. aerodenitrificans</i> SCSIO 75817<sup>T</sup>. When it was aerobically cultivated at 28 °C using NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N and NO<sub>2</sub><sup>−</sup>-N as the sole nitrogen sources, the nitrogen removal efficiencies were 73.4, 55.27 and 49.2%, respectively, and the maximum removal rates were 3.05, 1.82 and 1.63 mg/L/h, respectively. The results suggest that it has promising potential for wastewater treatment.https://www.mdpi.com/2076-2607/11/6/1532<i>Paracoccus</i> novel speciespolyphasic characterizationheterotrophic nitrification–aerobic denitrification |
| spellingShingle | Kun Zhang Qi Zeng Rouyun Jiang Songbiao Shi Jian Yang Lijuan Long Xinpeng Tian Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification Microorganisms <i>Paracoccus</i> novel species polyphasic characterization heterotrophic nitrification–aerobic denitrification |
| title | Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification |
| title_full | Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification |
| title_fullStr | Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification |
| title_full_unstemmed | Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification |
| title_short | Three Novel Marine Species of <i>Paracoccus</i>, <i>P. aerodenitrificans</i> sp. nov., <i>P. sediminicola</i> sp. nov. and <i>P. albus</i> sp. nov., and the Characterization of Their Capability to Perform Heterotrophic Nitrification and Aerobic Denitrification |
| title_sort | three novel marine species of i paracoccus i i p aerodenitrificans i sp nov i p sediminicola i sp nov and i p albus i sp nov and the characterization of their capability to perform heterotrophic nitrification and aerobic denitrification |
| topic | <i>Paracoccus</i> novel species polyphasic characterization heterotrophic nitrification–aerobic denitrification |
| url | https://www.mdpi.com/2076-2607/11/6/1532 |
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