Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
Abstract Background Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technol...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-019-1827-1 |
| _version_ | 1811206076001943552 |
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| author | Rui Fang Weimin Yang Xin Zhao Fang Xiong Caiqing Guo Jianping Xiao Li Chen Xiaoqing Song Honghua Wang Jie Chen Xiao Xiao Bing Yao Li-Yi Cai |
| author_facet | Rui Fang Weimin Yang Xin Zhao Fang Xiong Caiqing Guo Jianping Xiao Li Chen Xiaoqing Song Honghua Wang Jie Chen Xiao Xiao Bing Yao Li-Yi Cai |
| author_sort | Rui Fang |
| collection | DOAJ |
| description | Abstract Background Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. Methods A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. Results A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. Conclusions NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes. |
| first_indexed | 2024-04-12T03:41:50Z |
| format | Article |
| id | doaj.art-27eecda2d13c408699a085ccc727c37b |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-12T03:41:50Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-27eecda2d13c408699a085ccc727c37b2022-12-22T03:49:15ZengBMCJournal of Translational Medicine1479-58762019-03-011711810.1186/s12967-019-1827-1Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical studyRui Fang0Weimin Yang1Xin Zhao2Fang Xiong3Caiqing Guo4Jianping Xiao5Li Chen6Xiaoqing Song7Honghua Wang8Jie Chen9Xiao Xiao10Bing Yao11Li-Yi Cai12Centre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Hebei Maternity and Reproductive HospitalWuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityWuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityReproductive Medical Center of Nanjing Jinling Hospital and the Collaborative Innovation Platform for Reproductive Biology and Technology, Nanjing University School of MedicineCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityReproductive Medical Center of Nanjing Jinling Hospital and the Collaborative Innovation Platform for Reproductive Biology and Technology, Nanjing University School of MedicineCentre for Reproductive Medicine, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical UniversityAbstract Background Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology. Methods A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. Results A total of 413 embryos were obtained; 170 blastocysts were subjected to NICS. The screening showed euploidy in 79 embryos, aneuploidy in 52 embryos, and mosaic ploidy for 33 embryos. The rate of euploidy was comparable in couples with normal karyotype (50.7%; 38/75) vs. chromosomal rearrangement (43.2%; 41/95). A total of 52 euploid embryos (50 oocyte retrieval cycles) were transferred in 43 women. Biochemical pregnancy rate was 72.0% (36/50). Clinical pregnancy rate was 58.0% (29/50). The rate of spontaneous miscarriage was 3/29 (none with chromosomal aneuploidy). A total of 27 healthy babies were delivered. Conclusions NICS could identify embryo chromosomal abnormalities in couples either with or without chromosomal rearrangement, with satisfying clinical outcomes.http://link.springer.com/article/10.1186/s12967-019-1827-1Non-invasive chromosome screeningAssisted reproductive technologyChromosomal ploidyNext-generation sequencingClinical outcome |
| spellingShingle | Rui Fang Weimin Yang Xin Zhao Fang Xiong Caiqing Guo Jianping Xiao Li Chen Xiaoqing Song Honghua Wang Jie Chen Xiao Xiao Bing Yao Li-Yi Cai Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study Journal of Translational Medicine Non-invasive chromosome screening Assisted reproductive technology Chromosomal ploidy Next-generation sequencing Clinical outcome |
| title | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
| title_full | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
| title_fullStr | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
| title_full_unstemmed | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
| title_short | Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study |
| title_sort | chromosome screening using culture medium of embryos fertilised in vitro a pilot clinical study |
| topic | Non-invasive chromosome screening Assisted reproductive technology Chromosomal ploidy Next-generation sequencing Clinical outcome |
| url | http://link.springer.com/article/10.1186/s12967-019-1827-1 |
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