The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model
Despite the identification of several innovative targets for avoiding cognitive decline, there has yet to be a widely accepted approach that deals with minimising the deterioration of cognitive function. In this light, recent studies suggest that regulating the gut-brain axis with probiotics is a po...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12281 |
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| author | Sara Ferrari Rebecca Galla Simone Mulè Giorgia Rosso Arianna Brovero Valentina Macchi Sara Ruga Francesca Uberti |
| author_facet | Sara Ferrari Rebecca Galla Simone Mulè Giorgia Rosso Arianna Brovero Valentina Macchi Sara Ruga Francesca Uberti |
| author_sort | Sara Ferrari |
| collection | DOAJ |
| description | Despite the identification of several innovative targets for avoiding cognitive decline, there has yet to be a widely accepted approach that deals with minimising the deterioration of cognitive function. In this light, recent studies suggest that regulating the gut-brain axis with probiotics is a potential therapeutic strategy to support brain health. For this reason, in vitro models were used to examine the efficacy of different probiotic combinations to enhance intestinal homeostasis and positively affect the brain. Therefore, the new formulation has been evaluated for its capacity to modify intestinal barrier functions in a 3D in vitro model without any adverse effects and directly impact the mechanisms underlying cognitive function in a gut-brain axis model. According to our findings, <i>B. bifidum</i> novaBBF7 10 mg/mL, <i>B. longum</i> novaBLG2 5 mg/mL and <i>L. paracasei</i> TJB8 10 mg/mL may successfully modify the intestinal barrier and improve SCFA production. Successively, the probiotics studied caused no harm at the neuronal level, as demonstrated by iNOS, mitochondrial potential, and cell viability tests, confirming their safety features and enhancing antioxidant mechanisms and antineuroinflammation activity. Additionally, the damage caused by oxidative stress was also healed, and critical pathways that result in cognitive impairment were changed by synergetic action, supporting the hypothesis that brain ageing and neurodegeneration are slowed down. All these findings demonstrate the ability of probiotics to affect cognitive processes and their ability to sustain the mechanisms underlying cognitive function by acting on intestinal function. |
| first_indexed | 2024-03-11T00:25:10Z |
| format | Article |
| id | doaj.art-27ef870489d74376956995332498d2c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:25:10Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-27ef870489d74376956995332498d2c42023-11-18T23:02:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124151228110.3390/ijms241512281The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis ModelSara Ferrari0Rebecca Galla1Simone Mulè2Giorgia Rosso3Arianna Brovero4Valentina Macchi5Sara Ruga6Francesca Uberti7Laboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyNoivita Srls, Spin Off, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyLaboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyDespite the identification of several innovative targets for avoiding cognitive decline, there has yet to be a widely accepted approach that deals with minimising the deterioration of cognitive function. In this light, recent studies suggest that regulating the gut-brain axis with probiotics is a potential therapeutic strategy to support brain health. For this reason, in vitro models were used to examine the efficacy of different probiotic combinations to enhance intestinal homeostasis and positively affect the brain. Therefore, the new formulation has been evaluated for its capacity to modify intestinal barrier functions in a 3D in vitro model without any adverse effects and directly impact the mechanisms underlying cognitive function in a gut-brain axis model. According to our findings, <i>B. bifidum</i> novaBBF7 10 mg/mL, <i>B. longum</i> novaBLG2 5 mg/mL and <i>L. paracasei</i> TJB8 10 mg/mL may successfully modify the intestinal barrier and improve SCFA production. Successively, the probiotics studied caused no harm at the neuronal level, as demonstrated by iNOS, mitochondrial potential, and cell viability tests, confirming their safety features and enhancing antioxidant mechanisms and antineuroinflammation activity. Additionally, the damage caused by oxidative stress was also healed, and critical pathways that result in cognitive impairment were changed by synergetic action, supporting the hypothesis that brain ageing and neurodegeneration are slowed down. All these findings demonstrate the ability of probiotics to affect cognitive processes and their ability to sustain the mechanisms underlying cognitive function by acting on intestinal function.https://www.mdpi.com/1422-0067/24/15/12281cognitive declinegut-brain axisprobioticoral supplementationgut microbiome |
| spellingShingle | Sara Ferrari Rebecca Galla Simone Mulè Giorgia Rosso Arianna Brovero Valentina Macchi Sara Ruga Francesca Uberti The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model International Journal of Molecular Sciences cognitive decline gut-brain axis probiotic oral supplementation gut microbiome |
| title | The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model |
| title_full | The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model |
| title_fullStr | The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model |
| title_full_unstemmed | The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model |
| title_short | The Role of <i>Bifidobacterium bifidum</i> novaBBF7, <i>Bifidobacterium longum</i> novaBLG2 and <i>Lactobacillus paracasei</i> TJB8 to Improve Mechanisms Linked to Neuronal Cells Protection against Oxidative Condition in a Gut-Brain Axis Model |
| title_sort | role of i bifidobacterium bifidum i novabbf7 i bifidobacterium longum i novablg2 and i lactobacillus paracasei i tjb8 to improve mechanisms linked to neuronal cells protection against oxidative condition in a gut brain axis model |
| topic | cognitive decline gut-brain axis probiotic oral supplementation gut microbiome |
| url | https://www.mdpi.com/1422-0067/24/15/12281 |
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