Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells
The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor fo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2015-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/08413 |
| _version_ | 1828166355211255808 |
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| author | Nathan Kumar Jenna Richter Josh Cutts Kevin T Bush Cleber Trujillo Sanjay K Nigam Terry Gaasterland David Brafman Karl Willert |
| author_facet | Nathan Kumar Jenna Richter Josh Cutts Kevin T Bush Cleber Trujillo Sanjay K Nigam Terry Gaasterland David Brafman Karl Willert |
| author_sort | Nathan Kumar |
| collection | DOAJ |
| description | The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate. |
| first_indexed | 2024-04-12T02:00:00Z |
| format | Article |
| id | doaj.art-27f020729a6c4f04b5fe3dcd04b64c52 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:00:00Z |
| publishDate | 2015-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-27f020729a6c4f04b5fe3dcd04b64c522022-12-22T03:52:41ZengeLife Sciences Publications LtdeLife2050-084X2015-11-01410.7554/eLife.08413Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cellsNathan Kumar0Jenna Richter1Josh Cutts2Kevin T Bush3https://orcid.org/0000-0001-5889-2847Cleber Trujillo4Sanjay K Nigam5Terry Gaasterland6David Brafman7Karl Willert8Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United StatesDepartment of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United StatesSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, United StatesDepartment of Pediatrics, University of California, San Diego, San Diego, United StatesDepartment of Pediatrics, University of California, San Diego, San Diego, United StatesDepartment of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States; Department of Pediatrics, University of California, San Diego, San Diego, United StatesScripps Institution of Oceanography, Scripps Genome Center, University of California, San Diego, San Diego, United StatesSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, United StatesDepartment of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United StatesThe field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.https://elifesciences.org/articles/08413pluripotent stem cellintermediate mesodermdifferentiationarrayed cellular microenvironment |
| spellingShingle | Nathan Kumar Jenna Richter Josh Cutts Kevin T Bush Cleber Trujillo Sanjay K Nigam Terry Gaasterland David Brafman Karl Willert Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells eLife pluripotent stem cell intermediate mesoderm differentiation arrayed cellular microenvironment |
| title | Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| title_full | Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| title_fullStr | Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| title_full_unstemmed | Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| title_short | Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| title_sort | generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells |
| topic | pluripotent stem cell intermediate mesoderm differentiation arrayed cellular microenvironment |
| url | https://elifesciences.org/articles/08413 |
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