Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens
In this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in naturaand in the commercially available form. The antibacterial acti...
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| Format: | Article |
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Academia Brasileira de Ciências
2015-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100147&lng=en&tlng=en |
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| author | Luís F. Leandro Carlos A. Mendes Luciana A. Casemiro Adriana H.C. Vinholis Wilson R. Cunha Rosana de Almeida Carlos H.G. Martins |
| author_facet | Luís F. Leandro Carlos A. Mendes Luciana A. Casemiro Adriana H.C. Vinholis Wilson R. Cunha Rosana de Almeida Carlos H.G. Martins |
| author_sort | Luís F. Leandro |
| collection | DOAJ |
| description | In this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in naturaand in the commercially available form. The antibacterial actions of the main components of this apitoxin, phospholipase A2, and melittin were also assessed, alone and in combination. The following bacteria were tested: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, Lactobacillus casei, and Enterococcus faecalis. The MIC results obtained for the commercially available apitoxin and for the apitoxin in natura were close and lay between 20 and 40µg / mL, which indicated good antibacterial activity. Melittin was the most active component in apitoxin; it displayed very promising MIC values, from 4 to 40µg / mL. Phospholipase A2 presented MIC values higher than 400µg / mL. Association of mellitin with phospholipase A2 yielded MIC values ranging between 6 and 80µg / mL. Considering that tooth decay affects people's health, apitoxin and its component melittin have potential application against oral pathogens. |
| first_indexed | 2024-12-14T20:23:33Z |
| format | Article |
| id | doaj.art-27f36dfc6c224d1d88671c1c6bafb9c7 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-14T20:23:33Z |
| publishDate | 2015-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-27f36dfc6c224d1d88671c1c6bafb9c72022-12-21T22:48:40ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-03-0187114715510.1590/0001-3765201520130511S0001-37652015000100147Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogensLuís F. LeandroCarlos A. MendesLuciana A. CasemiroAdriana H.C. VinholisWilson R. CunhaRosana de AlmeidaCarlos H.G. MartinsIn this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in naturaand in the commercially available form. The antibacterial actions of the main components of this apitoxin, phospholipase A2, and melittin were also assessed, alone and in combination. The following bacteria were tested: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, Lactobacillus casei, and Enterococcus faecalis. The MIC results obtained for the commercially available apitoxin and for the apitoxin in natura were close and lay between 20 and 40µg / mL, which indicated good antibacterial activity. Melittin was the most active component in apitoxin; it displayed very promising MIC values, from 4 to 40µg / mL. Phospholipase A2 presented MIC values higher than 400µg / mL. Association of mellitin with phospholipase A2 yielded MIC values ranging between 6 and 80µg / mL. Considering that tooth decay affects people's health, apitoxin and its component melittin have potential application against oral pathogens.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100147&lng=en&tlng=enatividade antibacterianaapitoxinaMelitinaFosfolipase A2cárie dentalpatógenos bucais |
| spellingShingle | Luís F. Leandro Carlos A. Mendes Luciana A. Casemiro Adriana H.C. Vinholis Wilson R. Cunha Rosana de Almeida Carlos H.G. Martins Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens Anais da Academia Brasileira de Ciências atividade antibacteriana apitoxina Melitina Fosfolipase A2 cárie dental patógenos bucais |
| title | Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens |
| title_full | Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens |
| title_fullStr | Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens |
| title_full_unstemmed | Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens |
| title_short | Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens |
| title_sort | antimicrobial activity of apitoxin melittin and phospholipase a2 of honey bee apis mellifera venom against oral pathogens |
| topic | atividade antibacteriana apitoxina Melitina Fosfolipase A2 cárie dental patógenos bucais |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100147&lng=en&tlng=en |
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