Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity
ABSTRACTE-cadherin-catenin complex together with the cytoskeleton, builds the core of Adherens junctions (AJs). It has been reported that Scribble stabilizes the coupling of E-cadherin with catenins promoting epithelial cell adhesion, but the mechanism remains unknown. We show that Scribble, Lgl1, a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2023.2260645 |
| _version_ | 1797435027066191872 |
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| author | Maha Abedrabbo Shirel Sloomy Reham Abu-Leil Einav Kfir-Cohen Shoshana Ravid |
| author_facet | Maha Abedrabbo Shirel Sloomy Reham Abu-Leil Einav Kfir-Cohen Shoshana Ravid |
| author_sort | Maha Abedrabbo |
| collection | DOAJ |
| description | ABSTRACTE-cadherin-catenin complex together with the cytoskeleton, builds the core of Adherens junctions (AJs). It has been reported that Scribble stabilizes the coupling of E-cadherin with catenins promoting epithelial cell adhesion, but the mechanism remains unknown. We show that Scribble, Lgl1, and NMII-A reside in a complex with E-cadherin-catenin complex. Depletion of either Scribble or Lgl1 disrupts the localization of E-cadherin-catenin complex to AJs. aPKCζ phosphorylation of Lgl1 regulates AJ localization of Lgl1 and E-cadherin-catenin complexes. Both Scribble and Lgl1 regulate the activation and recruitment of NMII-A at AJs. Finally, Scribble and Lgl1 are downregulated by TGFβ-induced EMT, and their re-expression during EMT impedes its progression. Our results provide insight into the mechanism regulating AJ integrity by Scribble, Lgl1, and NMII-A. |
| first_indexed | 2024-03-09T10:42:19Z |
| format | Article |
| id | doaj.art-27f63c96dc4547c786dc8da988cebd25 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-03-09T10:42:19Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-27f63c96dc4547c786dc8da988cebd252023-12-01T13:29:40ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262023-12-0117112310.1080/19336918.2023.2260645Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrityMaha Abedrabbo0Shirel Sloomy1Reham Abu-Leil2Einav Kfir-CohenShoshana Ravid3Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, IsraelDepartment of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, IsraelDepartment of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, IsraelDepartment of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, IsraelABSTRACTE-cadherin-catenin complex together with the cytoskeleton, builds the core of Adherens junctions (AJs). It has been reported that Scribble stabilizes the coupling of E-cadherin with catenins promoting epithelial cell adhesion, but the mechanism remains unknown. We show that Scribble, Lgl1, and NMII-A reside in a complex with E-cadherin-catenin complex. Depletion of either Scribble or Lgl1 disrupts the localization of E-cadherin-catenin complex to AJs. aPKCζ phosphorylation of Lgl1 regulates AJ localization of Lgl1 and E-cadherin-catenin complexes. Both Scribble and Lgl1 regulate the activation and recruitment of NMII-A at AJs. Finally, Scribble and Lgl1 are downregulated by TGFβ-induced EMT, and their re-expression during EMT impedes its progression. Our results provide insight into the mechanism regulating AJ integrity by Scribble, Lgl1, and NMII-A.https://www.tandfonline.com/doi/10.1080/19336918.2023.2260645Adherens junctioncell adhesioncell polarityEMTmyosintumor suppressor |
| spellingShingle | Maha Abedrabbo Shirel Sloomy Reham Abu-Leil Einav Kfir-Cohen Shoshana Ravid Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity Cell Adhesion & Migration Adherens junction cell adhesion cell polarity EMT myosin tumor suppressor |
| title | Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity |
| title_full | Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity |
| title_fullStr | Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity |
| title_full_unstemmed | Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity |
| title_short | Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity |
| title_sort | scribble lgl1 and myosin iia interact with α β catenin to maintain epithelial junction integrity |
| topic | Adherens junction cell adhesion cell polarity EMT myosin tumor suppressor |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2023.2260645 |
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