Examination of Impact of Arsenic Stress on Soil Microbes

The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10...

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Bibliographic Details
Main Authors: Rashida Sultana, Maleeha Umber, Fareeha Iffat, Shanzah Kiran, Ayesha Khalid, Mirza Naseer Ahmad
Format: Article
Language:English
Published: National Centre of Excellence in Analytical Chemsitry 2020-12-01
Series:Pakistan Journal of Analytical & Environmental Chemistry
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Online Access:http://www.pjaec.pk/index.php/pjaec/article/view/592
Description
Summary:The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10 mg/L). The research was based on completely randomized design. Three drops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient) media to determine zone of inhibition through disk, well and spread method, while control group was without sodium arsenite application. After application of various levels of arsenic stress organisms were incubated for 24 h at 37°C. After incubation, zones of inhibition were measured. Our study has shown that both micobes cannot overcome higher levels of arsenic stress because in higher stress petriplates, increased inhibitory zones were observed.
ISSN:1996-918X
2221-5255