Examination of Impact of Arsenic Stress on Soil Microbes
The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10...
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| Format: | Article |
| Language: | English |
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National Centre of Excellence in Analytical Chemsitry
2020-12-01
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| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
| Subjects: | |
| Online Access: | http://www.pjaec.pk/index.php/pjaec/article/view/592 |
| _version_ | 1818560700227780608 |
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| author | Rashida Sultana Maleeha Umber Fareeha Iffat Shanzah Kiran Ayesha Khalid Mirza Naseer Ahmad |
| author_facet | Rashida Sultana Maleeha Umber Fareeha Iffat Shanzah Kiran Ayesha Khalid Mirza Naseer Ahmad |
| author_sort | Rashida Sultana |
| collection | DOAJ |
| description | The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10 mg/L). The research was based on completely randomized design. Three drops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient) media to determine zone of inhibition through disk, well and spread method, while control group was without sodium arsenite application. After application of various levels of arsenic stress organisms were incubated for 24 h at 37°C. After incubation, zones of inhibition were measured. Our study has shown that both micobes cannot overcome higher levels of arsenic stress because in higher stress petriplates, increased inhibitory zones were observed. |
| first_indexed | 2024-12-14T00:41:38Z |
| format | Article |
| id | doaj.art-27f7eb7bb84d471b8aa48f8cd66cd0af |
| institution | Directory Open Access Journal |
| issn | 1996-918X 2221-5255 |
| language | English |
| last_indexed | 2024-12-14T00:41:38Z |
| publishDate | 2020-12-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
| record_format | Article |
| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj.art-27f7eb7bb84d471b8aa48f8cd66cd0af2022-12-21T23:24:20ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552020-12-01212342348doi.org/10.21743/pjaec/2020.12.36Examination of Impact of Arsenic Stress on Soil MicrobesRashida Sultana0Maleeha Umber1Fareeha Iffat2Shanzah Kiran3Ayesha Khalid4Mirza Naseer Ahmad5Department of Botany, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.Department of Botany, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.Department of Botany, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.Department of Botany, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.Department of Botany, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.Department of Geography, Faculty of Earth Sciences, Abdus Salaam Research Forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10 mg/L). The research was based on completely randomized design. Three drops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient) media to determine zone of inhibition through disk, well and spread method, while control group was without sodium arsenite application. After application of various levels of arsenic stress organisms were incubated for 24 h at 37°C. After incubation, zones of inhibition were measured. Our study has shown that both micobes cannot overcome higher levels of arsenic stress because in higher stress petriplates, increased inhibitory zones were observed.http://www.pjaec.pk/index.php/pjaec/article/view/592pseudomonaspenicilliumarseniccledsoil microbeswell methodspread method |
| spellingShingle | Rashida Sultana Maleeha Umber Fareeha Iffat Shanzah Kiran Ayesha Khalid Mirza Naseer Ahmad Examination of Impact of Arsenic Stress on Soil Microbes Pakistan Journal of Analytical & Environmental Chemistry pseudomonas penicillium arsenic cled soil microbes well method spread method |
| title | Examination of Impact of Arsenic Stress on Soil Microbes |
| title_full | Examination of Impact of Arsenic Stress on Soil Microbes |
| title_fullStr | Examination of Impact of Arsenic Stress on Soil Microbes |
| title_full_unstemmed | Examination of Impact of Arsenic Stress on Soil Microbes |
| title_short | Examination of Impact of Arsenic Stress on Soil Microbes |
| title_sort | examination of impact of arsenic stress on soil microbes |
| topic | pseudomonas penicillium arsenic cled soil microbes well method spread method |
| url | http://www.pjaec.pk/index.php/pjaec/article/view/592 |
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