Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation
Micron-scale structure biphasic calcium phosphate (BCP) materials have demonstrated promising clinical outcomes in the field of bone tissue repair. However, research on biphasic calcium phosphate materials at the nanoscale level remains limited. In this study, we synthesize granular-shaped biphasic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1236429/full |
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| author | Yi-Chun Su Trinh T. T. Phan Tzu-Wei Wang Shao-Hsuan Chang Shao-Hsuan Chang Feng-Huei Lin Feng-Huei Lin Tzu-Sheng Hsu Lih-Yuan Lin |
| author_facet | Yi-Chun Su Trinh T. T. Phan Tzu-Wei Wang Shao-Hsuan Chang Shao-Hsuan Chang Feng-Huei Lin Feng-Huei Lin Tzu-Sheng Hsu Lih-Yuan Lin |
| author_sort | Yi-Chun Su |
| collection | DOAJ |
| description | Micron-scale structure biphasic calcium phosphate (BCP) materials have demonstrated promising clinical outcomes in the field of bone tissue repair. However, research on biphasic calcium phosphate materials at the nanoscale level remains limited. In this study, we synthesize granular-shaped biphasic calcium phosphate nanomaterials with multiple desirable characteristics, including negatively charged surfaces, non-cytotoxicity, and the capability to penetrate cells, using a nanogrinding dispersion process with a polymeric carboxylic acid as the dispersant. Our results reveal that treating human osteoblasts with 0.5 μg/mL biphasic calcium phosphate nanomaterials results in a marked increase in alkaline phosphatase (ALP) activity and the upregulation of osteogenesis-related genes. Furthermore, these biphasic calcium phosphate nanomaterials exhibit immunomodulatory properties. Treatment of THP-1-derived macrophages with BCP nanomaterials decreases the expression of various inflammatory genes. Biphasic calcium phosphate nanomaterials also mitigate the elevated inflammatory gene expression and protein production triggered by lipopolysaccharide (LPS) exposure in THP-1-derived macrophages. Notably, we observe that biphasic calcium phosphate nanomaterials have the capacity to reverse the detrimental effects of LPS-stimulated macrophage-conditioned medium on osteoblastic activity and mineralization. These findings underscore the potential utility of biphasic calcium phosphate nanomaterials in clinical settings for the repair and regeneration of bone tissue. In conclusion, this study highlights the material properties and positive effects of biphasic calcium phosphate nanomaterials on osteogenesis and immune regulation, opening a promising avenue for further research on inflammatory osteolysis in patients undergoing clinical surgery. |
| first_indexed | 2024-03-09T14:12:32Z |
| format | Article |
| id | doaj.art-27fa8d0173314342ac82e6678026d964 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-09T14:12:32Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-27fa8d0173314342ac82e6678026d9642023-11-29T05:54:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-11-011110.3389/fbioe.2023.12364291236429Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammationYi-Chun Su0Trinh T. T. Phan1Tzu-Wei Wang2Shao-Hsuan Chang3Shao-Hsuan Chang4Feng-Huei Lin5Feng-Huei Lin6Tzu-Sheng Hsu7Lih-Yuan Lin8Institute of Molecular and Cellular Biology, College of Life Sciences and Medicine, National Tsing Hua University, Hsinchu, TaiwanInstitute of Molecular and Cellular Biology, College of Life Sciences and Medicine, National Tsing Hua University, Hsinchu, TaiwanDepartment of Materials Science and Engineering, National Tsing Hua University, Hsinchu, TaiwanSchool of Engineering, University of Liverpool, Liverpool, United KingdomProfessional Master’s Program of Biotechnology Management, National Taiwan University, Taipei, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei, TaiwanInstitute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, TaiwanInstitute of Molecular and Cellular Biology, College of Life Sciences and Medicine, National Tsing Hua University, Hsinchu, TaiwanInstitute of Molecular and Cellular Biology, College of Life Sciences and Medicine, National Tsing Hua University, Hsinchu, TaiwanMicron-scale structure biphasic calcium phosphate (BCP) materials have demonstrated promising clinical outcomes in the field of bone tissue repair. However, research on biphasic calcium phosphate materials at the nanoscale level remains limited. In this study, we synthesize granular-shaped biphasic calcium phosphate nanomaterials with multiple desirable characteristics, including negatively charged surfaces, non-cytotoxicity, and the capability to penetrate cells, using a nanogrinding dispersion process with a polymeric carboxylic acid as the dispersant. Our results reveal that treating human osteoblasts with 0.5 μg/mL biphasic calcium phosphate nanomaterials results in a marked increase in alkaline phosphatase (ALP) activity and the upregulation of osteogenesis-related genes. Furthermore, these biphasic calcium phosphate nanomaterials exhibit immunomodulatory properties. Treatment of THP-1-derived macrophages with BCP nanomaterials decreases the expression of various inflammatory genes. Biphasic calcium phosphate nanomaterials also mitigate the elevated inflammatory gene expression and protein production triggered by lipopolysaccharide (LPS) exposure in THP-1-derived macrophages. Notably, we observe that biphasic calcium phosphate nanomaterials have the capacity to reverse the detrimental effects of LPS-stimulated macrophage-conditioned medium on osteoblastic activity and mineralization. These findings underscore the potential utility of biphasic calcium phosphate nanomaterials in clinical settings for the repair and regeneration of bone tissue. In conclusion, this study highlights the material properties and positive effects of biphasic calcium phosphate nanomaterials on osteogenesis and immune regulation, opening a promising avenue for further research on inflammatory osteolysis in patients undergoing clinical surgery.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1236429/fullnanoscaled biphasic calcium phosphate nanomaterialsbiphasic materialsmacrophagesosteoblastsinflammatory responseimmunomodulation |
| spellingShingle | Yi-Chun Su Trinh T. T. Phan Tzu-Wei Wang Shao-Hsuan Chang Shao-Hsuan Chang Feng-Huei Lin Feng-Huei Lin Tzu-Sheng Hsu Lih-Yuan Lin Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation Frontiers in Bioengineering and Biotechnology nanoscaled biphasic calcium phosphate nanomaterials biphasic materials macrophages osteoblasts inflammatory response immunomodulation |
| title | Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation |
| title_full | Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation |
| title_fullStr | Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation |
| title_full_unstemmed | Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation |
| title_short | Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation |
| title_sort | nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates lps induced inflammation |
| topic | nanoscaled biphasic calcium phosphate nanomaterials biphasic materials macrophages osteoblasts inflammatory response immunomodulation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1236429/full |
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