Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats
The mechanisms underlying the crosstalk between periodontitis and osteoporosis remain unclear. Recently, the gut microbiota has been recognized as a pivotal regulator of bone metabolism, and oral and gut mucosae are microbiologically connected. In this study, we investigated the effects of periodont...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.983608/full |
| _version_ | 1811310405600935936 |
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| author | Nannan Wang Lichun Zheng Jun Qian Min Wang Lili Li Yuezhen Huang Qian Zhang Yanfen Li Fuhua Yan |
| author_facet | Nannan Wang Lichun Zheng Jun Qian Min Wang Lili Li Yuezhen Huang Qian Zhang Yanfen Li Fuhua Yan |
| author_sort | Nannan Wang |
| collection | DOAJ |
| description | The mechanisms underlying the crosstalk between periodontitis and osteoporosis remain unclear. Recently, the gut microbiota has been recognized as a pivotal regulator of bone metabolism, and oral and gut mucosae are microbiologically connected. In this study, we investigated the effects of periodontitis on osteoporosis through the oral-gut axis. The salivary microbiota of patients with periodontitis was collected and then pumped into the intestine of Sprague–Dawley rats via intragastric administration for 2 weeks. An osteoporosis model was established using ovariectomy. Changes in the maxillae and femora were evaluated using microcomputed tomography (micro CT) and HE staining. Intestinal barrier integrity and inflammatory factors were examined using real-time quantitative polymerase chain reaction and immunofluorescence. The gut microbiota was profiled by 16S rRNA gene sequencing. Metabolome profiling of serum was performed using liquid chromatography-mass spectrometry sequencing. Micro CT and HE staining revealed osteoporotic phenotypes in the maxillae and femora of ovariectomized (OVX) rats. Our results confirmed that the salivary microbiota of patients with periodontitis aggravated femoral bone resorption in OVX rats. In addition, intestinal inflammation was exacerbated after periodontitis salivary microbiota gavage in OVX rats. Correlation analysis of microbiota and metabolomics revealed that lipolysis and tryptophan metabolism may be related to the bone loss induced by the salivary microbiota of patients with periodontitis. In conclusion, periodontitis can aggravate long bone loss through the oral-gut axis in OVX rats. |
| first_indexed | 2024-04-13T09:58:17Z |
| format | Article |
| id | doaj.art-27ff390257a24ecdb1095ec10d387d69 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T09:58:17Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-27ff390257a24ecdb1095ec10d387d692022-12-22T02:51:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.983608983608Salivary microbiota of periodontitis aggravates bone loss in ovariectomized ratsNannan WangLichun ZhengJun QianMin WangLili LiYuezhen HuangQian ZhangYanfen LiFuhua YanThe mechanisms underlying the crosstalk between periodontitis and osteoporosis remain unclear. Recently, the gut microbiota has been recognized as a pivotal regulator of bone metabolism, and oral and gut mucosae are microbiologically connected. In this study, we investigated the effects of periodontitis on osteoporosis through the oral-gut axis. The salivary microbiota of patients with periodontitis was collected and then pumped into the intestine of Sprague–Dawley rats via intragastric administration for 2 weeks. An osteoporosis model was established using ovariectomy. Changes in the maxillae and femora were evaluated using microcomputed tomography (micro CT) and HE staining. Intestinal barrier integrity and inflammatory factors were examined using real-time quantitative polymerase chain reaction and immunofluorescence. The gut microbiota was profiled by 16S rRNA gene sequencing. Metabolome profiling of serum was performed using liquid chromatography-mass spectrometry sequencing. Micro CT and HE staining revealed osteoporotic phenotypes in the maxillae and femora of ovariectomized (OVX) rats. Our results confirmed that the salivary microbiota of patients with periodontitis aggravated femoral bone resorption in OVX rats. In addition, intestinal inflammation was exacerbated after periodontitis salivary microbiota gavage in OVX rats. Correlation analysis of microbiota and metabolomics revealed that lipolysis and tryptophan metabolism may be related to the bone loss induced by the salivary microbiota of patients with periodontitis. In conclusion, periodontitis can aggravate long bone loss through the oral-gut axis in OVX rats.https://www.frontiersin.org/articles/10.3389/fcimb.2022.983608/fullperiodontitisosteoporosissalivary microbiotagut microbiotabone loss |
| spellingShingle | Nannan Wang Lichun Zheng Jun Qian Min Wang Lili Li Yuezhen Huang Qian Zhang Yanfen Li Fuhua Yan Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats Frontiers in Cellular and Infection Microbiology periodontitis osteoporosis salivary microbiota gut microbiota bone loss |
| title | Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| title_full | Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| title_fullStr | Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| title_full_unstemmed | Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| title_short | Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| title_sort | salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats |
| topic | periodontitis osteoporosis salivary microbiota gut microbiota bone loss |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.983608/full |
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