Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma
Background. Advanced glycation end products’ receptor (AGER) is a multiligand receptor that interacts with a wide range of ligands. Previous studies have shown that abnormal AGER expression is closely related to immune infiltration and tumorigenesis. However, the AGER DNA methylation relationship be...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi - Cambridge University Press
2023-01-01
|
| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2023/7129325 |
| _version_ | 1797772577638187008 |
|---|---|
| author | Jun Yang Mingqiang Lin Mengyan Zhang Zhiping Wang Hancui Lin Yilin Yu Qunhao Zheng Xiaohui Chen Yahua Wu Qiwei Yao Jiancheng Li |
| author_facet | Jun Yang Mingqiang Lin Mengyan Zhang Zhiping Wang Hancui Lin Yilin Yu Qunhao Zheng Xiaohui Chen Yahua Wu Qiwei Yao Jiancheng Li |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | Background. Advanced glycation end products’ receptor (AGER) is a multiligand receptor that interacts with a wide range of ligands. Previous studies have shown that abnormal AGER expression is closely related to immune infiltration and tumorigenesis. However, the AGER DNA methylation relationship between prognosis and infiltrating immune cells in LUAD and LUSC is still unclear. Methods. AGER expression in pan-cancer was obtained by using the UALCAN databases. Kaplan–Meier plotter showed the correlation of AGER mRNA expression levels and clinicopathological parameters. The protein expression levels for AGER were derived from Human Protein Atlas Database Analysis. The copy number, somatic mutation, and DNA methylation of AGER were presented with UCSC Xena database. TIMER platform and TISIDB website were used to show the correlation between AGER expression and tumor immune cell infiltration level. Results. The expression level of AGER was significantly reduced in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Low expression of AGER was significantly correlated with histology, stage, lymph node metastasis, and tumor protein 53 (TP53) mutation and could be used as a potential indicator of poor prognosis of LUAD and LUSC. Moreover, AGER expression was positively correlated with the infiltrating immune cells. Further analysis showed that copy number variation (CNV), mutation, and DNA methylation were involved in AGER downregulation. In addition, we also found that hypermethylated AGER was significantly correlated with tumor-infiltrating lymphocytes. Conclusion. AGER may be a candidate for the prognostic biomarker of LUAD and LUSC related to tumor immune microenvironment. |
| first_indexed | 2024-03-12T21:52:53Z |
| format | Article |
| id | doaj.art-28008b581d834e7fad4cabf900efed5f |
| institution | Directory Open Access Journal |
| issn | 1469-5073 |
| language | English |
| last_indexed | 2024-03-12T21:52:53Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi - Cambridge University Press |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj.art-28008b581d834e7fad4cabf900efed5f2023-07-26T00:00:00ZengHindawi - Cambridge University PressGenetics Research1469-50732023-01-01202310.1155/2023/7129325Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell CarcinomaJun Yang0Mingqiang Lin1Mengyan Zhang2Zhiping Wang3Hancui Lin4Yilin Yu5Qunhao Zheng6Xiaohui Chen7Yahua Wu8Qiwei Yao9Jiancheng Li10Department of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyClinical Oncology School of Fujian Medical UniversityDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyBackground. Advanced glycation end products’ receptor (AGER) is a multiligand receptor that interacts with a wide range of ligands. Previous studies have shown that abnormal AGER expression is closely related to immune infiltration and tumorigenesis. However, the AGER DNA methylation relationship between prognosis and infiltrating immune cells in LUAD and LUSC is still unclear. Methods. AGER expression in pan-cancer was obtained by using the UALCAN databases. Kaplan–Meier plotter showed the correlation of AGER mRNA expression levels and clinicopathological parameters. The protein expression levels for AGER were derived from Human Protein Atlas Database Analysis. The copy number, somatic mutation, and DNA methylation of AGER were presented with UCSC Xena database. TIMER platform and TISIDB website were used to show the correlation between AGER expression and tumor immune cell infiltration level. Results. The expression level of AGER was significantly reduced in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Low expression of AGER was significantly correlated with histology, stage, lymph node metastasis, and tumor protein 53 (TP53) mutation and could be used as a potential indicator of poor prognosis of LUAD and LUSC. Moreover, AGER expression was positively correlated with the infiltrating immune cells. Further analysis showed that copy number variation (CNV), mutation, and DNA methylation were involved in AGER downregulation. In addition, we also found that hypermethylated AGER was significantly correlated with tumor-infiltrating lymphocytes. Conclusion. AGER may be a candidate for the prognostic biomarker of LUAD and LUSC related to tumor immune microenvironment.http://dx.doi.org/10.1155/2023/7129325 |
| spellingShingle | Jun Yang Mingqiang Lin Mengyan Zhang Zhiping Wang Hancui Lin Yilin Yu Qunhao Zheng Xiaohui Chen Yahua Wu Qiwei Yao Jiancheng Li Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma Genetics Research |
| title | Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_full | Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_fullStr | Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_full_unstemmed | Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_short | Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_sort | advanced glycation end products receptor dna methylation associated with immune infiltration and prognosis of lung adenocarcinoma and lung squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2023/7129325 |
| work_keys_str_mv | AT junyang advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT mingqianglin advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT mengyanzhang advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT zhipingwang advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT hancuilin advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT yilinyu advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT qunhaozheng advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT xiaohuichen advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT yahuawu advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT qiweiyao advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma AT jianchengli advancedglycationendproductsreceptordnamethylationassociatedwithimmuneinfiltrationandprognosisoflungadenocarcinomaandlungsquamouscellcarcinoma |