Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication
3D bioprinting has become a versatile and powerful method in tissue engineering and regenerative medicine and is increasingly adapted by other disciplines due to its tremendous potential beyond its typical applications. However, commercially available 3D bioprinting systems are typically expensive c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00184/full |
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| author | Melanie Kahl Markus Gertig Phillipp Hoyer Oliver Friedrich Daniel F. Gilbert |
| author_facet | Melanie Kahl Markus Gertig Phillipp Hoyer Oliver Friedrich Daniel F. Gilbert |
| author_sort | Melanie Kahl |
| collection | DOAJ |
| description | 3D bioprinting has become a versatile and powerful method in tissue engineering and regenerative medicine and is increasingly adapted by other disciplines due to its tremendous potential beyond its typical applications. However, commercially available 3D bioprinting systems are typically expensive circumventing the broad implementation, including laboratories in low-resource settings. To address the limitations of conventional and commercially available technology, we developed a 3D bioprinter by modification of an off-the-shelf 3D desktop printer, that can be installed within a single day, is of handy size to fit into a standard laminar flow hood, customizable, ultra-low cost and thus, affordable to a broad range of research labs, or educational institutions. We evaluate accuracy and reproducibility of printing results using alginate and alginate/gelatin-hydrogels and demonstrate its potential for biomedical use by printing of various two-and three-dimensional cell-free and mammalian cell-laden objects using recombinant HEKYFP cells, stably expressing yellow fluorescent protein (YFP) as a model system and high-content imaging. We further provide a parts list and 3D design files in STL and STEP format for reconstructing the device. A time-lapse video of the custom-built device during operation is available at https://vimeo.com/274482794. |
| first_indexed | 2024-12-16T16:41:25Z |
| format | Article |
| id | doaj.art-2800d0ca53044b7c99692c1cc457cd79 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-16T16:41:25Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2800d0ca53044b7c99692c1cc457cd792022-12-21T22:24:18ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-07-01710.3389/fbioe.2019.00184464779Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for BiofabricationMelanie KahlMarkus GertigPhillipp HoyerOliver FriedrichDaniel F. Gilbert3D bioprinting has become a versatile and powerful method in tissue engineering and regenerative medicine and is increasingly adapted by other disciplines due to its tremendous potential beyond its typical applications. However, commercially available 3D bioprinting systems are typically expensive circumventing the broad implementation, including laboratories in low-resource settings. To address the limitations of conventional and commercially available technology, we developed a 3D bioprinter by modification of an off-the-shelf 3D desktop printer, that can be installed within a single day, is of handy size to fit into a standard laminar flow hood, customizable, ultra-low cost and thus, affordable to a broad range of research labs, or educational institutions. We evaluate accuracy and reproducibility of printing results using alginate and alginate/gelatin-hydrogels and demonstrate its potential for biomedical use by printing of various two-and three-dimensional cell-free and mammalian cell-laden objects using recombinant HEKYFP cells, stably expressing yellow fluorescent protein (YFP) as a model system and high-content imaging. We further provide a parts list and 3D design files in STL and STEP format for reconstructing the device. A time-lapse video of the custom-built device during operation is available at https://vimeo.com/274482794.https://www.frontiersin.org/article/10.3389/fbioe.2019.00184/fullultra-low-cost bioprinting technologyalginate and alginate/gelatin-based hydrogelHEK293YFPI152L cellsrapid prototyping3D desktop printing |
| spellingShingle | Melanie Kahl Markus Gertig Phillipp Hoyer Oliver Friedrich Daniel F. Gilbert Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication Frontiers in Bioengineering and Biotechnology ultra-low-cost bioprinting technology alginate and alginate/gelatin-based hydrogel HEK293YFPI152L cells rapid prototyping 3D desktop printing |
| title | Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication |
| title_full | Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication |
| title_fullStr | Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication |
| title_full_unstemmed | Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication |
| title_short | Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication |
| title_sort | ultra low cost 3d bioprinting modification and application of an off the shelf desktop 3d printer for biofabrication |
| topic | ultra-low-cost bioprinting technology alginate and alginate/gelatin-based hydrogel HEK293YFPI152L cells rapid prototyping 3D desktop printing |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00184/full |
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