The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.
The natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properti...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5094676?pdf=render |
| _version_ | 1818944439330013184 |
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| author | Marian Morales Vladimir Sentchilo Claire Bertelli Andrea Komljenovic Nadezda Kryuchkova-Mostacci Audrey Bourdilloud Burkhard Linke Alexander Goesmann Keith Harshman Francisca Segers Fabien Delapierre Damien Fiorucci Mathieu Seppey Evgeniya Trofimenco Pauline Berra Athimed El Taher Chloé Loiseau Dejan Roggero Madeleine Sulfiotti Angela Etienne Gustavo Ruiz Buendia Loïc Pillard Angelique Escoriza Roxane Moritz Cedric Schneider Esteban Alfonso Fatma Ben Jeddou Oliver Selmoni Gregory Resch Gilbert Greub Olivier Emery Manupriyam Dubey Trestan Pillonel Marc Robinson-Rechavi Jan Roelof van der Meer |
| author_facet | Marian Morales Vladimir Sentchilo Claire Bertelli Andrea Komljenovic Nadezda Kryuchkova-Mostacci Audrey Bourdilloud Burkhard Linke Alexander Goesmann Keith Harshman Francisca Segers Fabien Delapierre Damien Fiorucci Mathieu Seppey Evgeniya Trofimenco Pauline Berra Athimed El Taher Chloé Loiseau Dejan Roggero Madeleine Sulfiotti Angela Etienne Gustavo Ruiz Buendia Loïc Pillard Angelique Escoriza Roxane Moritz Cedric Schneider Esteban Alfonso Fatma Ben Jeddou Oliver Selmoni Gregory Resch Gilbert Greub Olivier Emery Manupriyam Dubey Trestan Pillonel Marc Robinson-Rechavi Jan Roelof van der Meer |
| author_sort | Marian Morales |
| collection | DOAJ |
| description | The natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. In order to better understand the adaptive response of cells during a transition to contaminated material, we analyzed here the genome and short-term (1 h) changes in genome-wide gene expression of the BTEX-degrading bacterium Pseudomonas veronii 1YdBTEX2 in non-sterile soil and liquid medium, both in presence or absence of toluene. We obtained a gapless genome sequence of P. veronii 1YdBTEX2 covering three individual replicons with a total size of 8 Mb, two of which are largely unrelated to current known bacterial replicons. One-hour exposure to toluene, both in soil and liquid, triggered massive transcription (up to 208-fold induction) of multiple gene clusters, such as toluene degradation pathway(s), chemotaxis and toluene efflux pumps. This clearly underlines their key role in the adaptive response to toluene. In comparison to liquid medium, cells in soil drastically changed expression of genes involved in membrane functioning (e.g., lipid composition, lipid metabolism, cell fatty acid synthesis), osmotic stress response (e.g., polyamine or trehalose synthesis, uptake of potassium) and putrescine metabolism, highlighting the immediate response mechanisms of P. veronii 1YdBTEX2 for successful establishment in polluted soil. |
| first_indexed | 2024-12-20T07:43:14Z |
| format | Article |
| id | doaj.art-28020bdb2be14e7c8e41d4fcfed3cdbd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:43:14Z |
| publishDate | 2016-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-28020bdb2be14e7c8e41d4fcfed3cdbd2022-12-21T19:48:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011111e016585010.1371/journal.pone.0165850The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.Marian MoralesVladimir SentchiloClaire BertelliAndrea KomljenovicNadezda Kryuchkova-MostacciAudrey BourdilloudBurkhard LinkeAlexander GoesmannKeith HarshmanFrancisca SegersFabien DelapierreDamien FiorucciMathieu SeppeyEvgeniya TrofimencoPauline BerraAthimed El TaherChloé LoiseauDejan RoggeroMadeleine SulfiottiAngela EtienneGustavo Ruiz BuendiaLoïc PillardAngelique EscorizaRoxane MoritzCedric SchneiderEsteban AlfonsoFatma Ben JeddouOliver SelmoniGregory ReschGilbert GreubOlivier EmeryManupriyam DubeyTrestan PillonelMarc Robinson-RechaviJan Roelof van der MeerThe natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. In order to better understand the adaptive response of cells during a transition to contaminated material, we analyzed here the genome and short-term (1 h) changes in genome-wide gene expression of the BTEX-degrading bacterium Pseudomonas veronii 1YdBTEX2 in non-sterile soil and liquid medium, both in presence or absence of toluene. We obtained a gapless genome sequence of P. veronii 1YdBTEX2 covering three individual replicons with a total size of 8 Mb, two of which are largely unrelated to current known bacterial replicons. One-hour exposure to toluene, both in soil and liquid, triggered massive transcription (up to 208-fold induction) of multiple gene clusters, such as toluene degradation pathway(s), chemotaxis and toluene efflux pumps. This clearly underlines their key role in the adaptive response to toluene. In comparison to liquid medium, cells in soil drastically changed expression of genes involved in membrane functioning (e.g., lipid composition, lipid metabolism, cell fatty acid synthesis), osmotic stress response (e.g., polyamine or trehalose synthesis, uptake of potassium) and putrescine metabolism, highlighting the immediate response mechanisms of P. veronii 1YdBTEX2 for successful establishment in polluted soil.http://europepmc.org/articles/PMC5094676?pdf=render |
| spellingShingle | Marian Morales Vladimir Sentchilo Claire Bertelli Andrea Komljenovic Nadezda Kryuchkova-Mostacci Audrey Bourdilloud Burkhard Linke Alexander Goesmann Keith Harshman Francisca Segers Fabien Delapierre Damien Fiorucci Mathieu Seppey Evgeniya Trofimenco Pauline Berra Athimed El Taher Chloé Loiseau Dejan Roggero Madeleine Sulfiotti Angela Etienne Gustavo Ruiz Buendia Loïc Pillard Angelique Escoriza Roxane Moritz Cedric Schneider Esteban Alfonso Fatma Ben Jeddou Oliver Selmoni Gregory Resch Gilbert Greub Olivier Emery Manupriyam Dubey Trestan Pillonel Marc Robinson-Rechavi Jan Roelof van der Meer The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. PLoS ONE |
| title | The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. |
| title_full | The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. |
| title_fullStr | The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. |
| title_full_unstemmed | The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. |
| title_short | The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. |
| title_sort | genome of the toluene degrading pseudomonas veronii strain 1ydbtex2 and its differential gene expression in contaminated sand |
| url | http://europepmc.org/articles/PMC5094676?pdf=render |
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