Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia
We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandul...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2018-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800022G.pdf |
| _version_ | 1819213333880897536 |
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| author | Gavrilović Milan Tešević Vele Đorđević Iris Rajčević Nemanja Bakhia Arsena Garcia Jacas Núria Susanna Alfonso Marin Petar D. Janaćković Peđa |
| author_facet | Gavrilović Milan Tešević Vele Đorđević Iris Rajčević Nemanja Bakhia Arsena Garcia Jacas Núria Susanna Alfonso Marin Petar D. Janaćković Peđa |
| author_sort | Gavrilović Milan |
| collection | DOAJ |
| description | We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2΄-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029 and Grant no. 172053] |
| first_indexed | 2024-12-23T06:57:12Z |
| format | Article |
| id | doaj.art-28039145dae340fdbc118145afbede83 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-23T06:57:12Z |
| publishDate | 2018-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-28039145dae340fdbc118145afbede832022-12-21T17:56:17ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392018-01-0170461362010.2298/ABS180309022G0354-46641800022GLeaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from GeorgiaGavrilović Milan0Tešević Vele1Đorđević Iris2Rajčević Nemanja3Bakhia Arsena4Garcia Jacas Núria5Susanna Alfonso6Marin Petar D.7Janaćković Peđa8Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeFaculty of Chemistry, BelgradeFaculty of Veterinary Medicine, BelgradeFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeIlia State University, School of Natural Sciences and Engineering, Tbilisi, GeorgiaBotanic Institute of Barcelona (IBB,CSIC-ICUB), Barcelona, SpainBotanic Institute of Barcelona (IBB,CSIC-ICUB), Barcelona, SpainFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeWe examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2΄-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029 and Grant no. 172053]http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800022G.pdfAmphoricarpos elegansglandular trichomes3-deoxyamphoricarpolideantioxidative activity |
| spellingShingle | Gavrilović Milan Tešević Vele Đorđević Iris Rajčević Nemanja Bakhia Arsena Garcia Jacas Núria Susanna Alfonso Marin Petar D. Janaćković Peđa Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia Archives of Biological Sciences Amphoricarpos elegans glandular trichomes 3-deoxyamphoricarpolide antioxidative activity |
| title | Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia |
| title_full | Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia |
| title_fullStr | Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia |
| title_full_unstemmed | Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia |
| title_short | Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia |
| title_sort | leaf micromorphology antioxidative activity and a new record of 3 deoxyamphoricarpolide of relict and limestone endemic amphoricarpos elegans albov compositae from georgia |
| topic | Amphoricarpos elegans glandular trichomes 3-deoxyamphoricarpolide antioxidative activity |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800022G.pdf |
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