Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
Calcium (Ca<sup>2+</sup>) plays essential roles in plant growth and development. Ca<sup>2+</sup> deficiency causes a physiological disorder of tip-burn in Brassiceae crops and is involved in the regulation of cellular Ca<sup>2+</sup> homeostasis. Although the func...
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MDPI AG
2023-09-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/9/1810 |
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| author | Shuning Cui Hong Liu Yong Wu Lugang Zhang Shanshan Nie |
| author_facet | Shuning Cui Hong Liu Yong Wu Lugang Zhang Shanshan Nie |
| author_sort | Shuning Cui |
| collection | DOAJ |
| description | Calcium (Ca<sup>2+</sup>) plays essential roles in plant growth and development. Ca<sup>2+</sup> deficiency causes a physiological disorder of tip-burn in Brassiceae crops and is involved in the regulation of cellular Ca<sup>2+</sup> homeostasis. Although the functions of Ca<sup>2+</sup>/H<sup>+</sup> exchanger antiporters (CAXs) in mediating transmembrane transport of Ca<sup>2+</sup> have been extensively characterized in multiple plant species, the potential roles of <i>BrCAX</i> genes remain unclear in Chinese cabbage. In this study, eight genes of the <i>BrCAX</i> family were genome-widely identified in Chinese cabbage. These BrCAX proteins contained conserved Na_Ca_ex domain and belonged to five members of the <i>CAX</i> family. Molecular evolutionary analysis and sequence alignment revealed the evolutionary conservation of <i>BrCAX</i> family genes. Expression profiling demonstrated that eight <i>BrCAX</i> genes exhibited differential expression in different tissues and under heat stress. Furthermore, Ca<sup>2+</sup> deficiency treatment induced the typical symptoms of tip-burn in Chinese cabbage seedlings and a significant decrease in total Ca<sup>2+</sup> content in both roots and leaves. The expression changes in <i>BrCAX</i> genes were related to the response to Ca<sup>2+</sup> deficiency-induced tip-burn of Chinese cabbage. Specially, <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes were highly expressed gene members of the <i>BrCAX</i> family in the leaves and were significantly differentially expressed under Ca<sup>2+</sup> deficiency stress. Moreover, overexpression of <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes in yeast and Chinese cabbage cotyledons exhibited a higher Ca<sup>2+</sup> tolerance, indicating the Ca<sup>2+</sup> transport capacity of <i>BrCAX1-1</i> and <i>BrCAX1-2</i>. In addition, suppression expression of <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes reduced cytosolic Ca<sup>2+</sup> levels in the root tips of Chinese cabbage. These results provide references for functional studies of <i>BrCAX</i> genes and to investigate the regulatory mechanisms underlying Ca<sup>2+</sup> deficiency disorder in Brassiceae vegetables. |
| first_indexed | 2024-03-10T22:43:06Z |
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| last_indexed | 2024-03-10T22:43:06Z |
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| spelling | doaj.art-2809de18ecde449ab8cea801921c56a52023-11-19T10:54:01ZengMDPI AGGenes2073-44252023-09-01149181010.3390/genes14091810Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)Shuning Cui0Hong Liu1Yong Wu2Lugang Zhang3Shanshan Nie4State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaCalcium (Ca<sup>2+</sup>) plays essential roles in plant growth and development. Ca<sup>2+</sup> deficiency causes a physiological disorder of tip-burn in Brassiceae crops and is involved in the regulation of cellular Ca<sup>2+</sup> homeostasis. Although the functions of Ca<sup>2+</sup>/H<sup>+</sup> exchanger antiporters (CAXs) in mediating transmembrane transport of Ca<sup>2+</sup> have been extensively characterized in multiple plant species, the potential roles of <i>BrCAX</i> genes remain unclear in Chinese cabbage. In this study, eight genes of the <i>BrCAX</i> family were genome-widely identified in Chinese cabbage. These BrCAX proteins contained conserved Na_Ca_ex domain and belonged to five members of the <i>CAX</i> family. Molecular evolutionary analysis and sequence alignment revealed the evolutionary conservation of <i>BrCAX</i> family genes. Expression profiling demonstrated that eight <i>BrCAX</i> genes exhibited differential expression in different tissues and under heat stress. Furthermore, Ca<sup>2+</sup> deficiency treatment induced the typical symptoms of tip-burn in Chinese cabbage seedlings and a significant decrease in total Ca<sup>2+</sup> content in both roots and leaves. The expression changes in <i>BrCAX</i> genes were related to the response to Ca<sup>2+</sup> deficiency-induced tip-burn of Chinese cabbage. Specially, <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes were highly expressed gene members of the <i>BrCAX</i> family in the leaves and were significantly differentially expressed under Ca<sup>2+</sup> deficiency stress. Moreover, overexpression of <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes in yeast and Chinese cabbage cotyledons exhibited a higher Ca<sup>2+</sup> tolerance, indicating the Ca<sup>2+</sup> transport capacity of <i>BrCAX1-1</i> and <i>BrCAX1-2</i>. In addition, suppression expression of <i>BrCAX1-1</i> and <i>BrCAX1-2</i> genes reduced cytosolic Ca<sup>2+</sup> levels in the root tips of Chinese cabbage. These results provide references for functional studies of <i>BrCAX</i> genes and to investigate the regulatory mechanisms underlying Ca<sup>2+</sup> deficiency disorder in Brassiceae vegetables.https://www.mdpi.com/2073-4425/14/9/1810Chinese cabbage<i>BrCAX</i> genesCa<sup>2+</sup> deficiency stresstip-burnexpression analysis |
| spellingShingle | Shuning Cui Hong Liu Yong Wu Lugang Zhang Shanshan Nie Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) Genes Chinese cabbage <i>BrCAX</i> genes Ca<sup>2+</sup> deficiency stress tip-burn expression analysis |
| title | Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) |
| title_full | Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) |
| title_fullStr | Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) |
| title_full_unstemmed | Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) |
| title_short | Genome-Wide Identification of <i>BrCAX</i> Genes and Functional Analysis of <i>BrCAX1</i> Involved in Ca<sup>2+</sup> Transport and Ca<sup>2+</sup> Deficiency-Induced Tip-Burn in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) |
| title_sort | genome wide identification of i brcax i genes and functional analysis of i brcax1 i involved in ca sup 2 sup transport and ca sup 2 sup deficiency induced tip burn in chinese cabbage i brassica rapa i l ssp i pekinensis i |
| topic | Chinese cabbage <i>BrCAX</i> genes Ca<sup>2+</sup> deficiency stress tip-burn expression analysis |
| url | https://www.mdpi.com/2073-4425/14/9/1810 |
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