Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture
There is increasing attention being paid to utilizing microbial communities to improve plant health while reducing management inputs. Thus, the objectives of this research were to assess changes in the rhizosphere bacterial community structure associated with long-term turfgrass monoculture and to d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.530067/full |
| _version_ | 1818960408936972288 |
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| author | Chang-Wook Jeon Da-Ran Kim Eun-Ji Bae Youn-Sig Kwak Youn-Sig Kwak |
| author_facet | Chang-Wook Jeon Da-Ran Kim Eun-Ji Bae Youn-Sig Kwak Youn-Sig Kwak |
| author_sort | Chang-Wook Jeon |
| collection | DOAJ |
| description | There is increasing attention being paid to utilizing microbial communities to improve plant health while reducing management inputs. Thus, the objectives of this research were to assess changes in the rhizosphere bacterial community structure associated with long-term turfgrass monoculture and to demonstrate the feasibility of using functional bacteria as beneficial biocontrol agents. Large patch disease, caused by the fungal pathogen Rhizoctonia solani AG2-2, is a significant threat to turfgrass cultivation. Rhizosphere samples were collected from 2-, 13- and 25-year turfgrass (Zoysia japonica) monocultures. The 13-year monoculture field had a higher pathogen population density than both the 2- and 25-year monoculture fields. Analyses of the rhizosphere bacterial communities revealed that Streptomyces was dominant in the 2-year field and Burkholderia was enriched in the 25-year field. Based on the culturable rhizosphere bacteria, Streptomyces neyagawaensis J6 and Burkholderia vietnamiensis J10 were obtained from the 2- and 25-year fields, respectively. Application of S. neyagawaensis J6 and B. vietnamiensis J10 led to excellent inhibition of large patch disease as well as enhanced tolerance against drought and temperature stresses. The results showed that the selected bacteria could be developed as biocontrol and abiotic stress tolerance agents for turfgrass cultivation. |
| first_indexed | 2024-12-20T11:57:04Z |
| format | Article |
| id | doaj.art-280b2cbd7f1c4830b88016298c6dfa6d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-20T11:57:04Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-280b2cbd7f1c4830b88016298c6dfa6d2022-12-21T19:41:38ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-01-01810.3389/fbioe.2020.530067530067Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass MonocultureChang-Wook Jeon0Da-Ran Kim1Eun-Ji Bae2Youn-Sig Kwak3Youn-Sig Kwak4Dvision of Applied Life Science (BK21 Plus) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, South KoreaDvision of Applied Life Science (BK21 Plus) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, South KoreaForest Biomaterials Research Center, National Institute of Forest Science, Jinju, South KoreaDvision of Applied Life Science (BK21 Plus) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, South KoreaDepartment of Plant Medicine, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, South KoreaThere is increasing attention being paid to utilizing microbial communities to improve plant health while reducing management inputs. Thus, the objectives of this research were to assess changes in the rhizosphere bacterial community structure associated with long-term turfgrass monoculture and to demonstrate the feasibility of using functional bacteria as beneficial biocontrol agents. Large patch disease, caused by the fungal pathogen Rhizoctonia solani AG2-2, is a significant threat to turfgrass cultivation. Rhizosphere samples were collected from 2-, 13- and 25-year turfgrass (Zoysia japonica) monocultures. The 13-year monoculture field had a higher pathogen population density than both the 2- and 25-year monoculture fields. Analyses of the rhizosphere bacterial communities revealed that Streptomyces was dominant in the 2-year field and Burkholderia was enriched in the 25-year field. Based on the culturable rhizosphere bacteria, Streptomyces neyagawaensis J6 and Burkholderia vietnamiensis J10 were obtained from the 2- and 25-year fields, respectively. Application of S. neyagawaensis J6 and B. vietnamiensis J10 led to excellent inhibition of large patch disease as well as enhanced tolerance against drought and temperature stresses. The results showed that the selected bacteria could be developed as biocontrol and abiotic stress tolerance agents for turfgrass cultivation.https://www.frontiersin.org/articles/10.3389/fbioe.2020.530067/fullantifungal activityBurkholderiamonocultureplant growth promotionStreptomycesturfgrass |
| spellingShingle | Chang-Wook Jeon Da-Ran Kim Eun-Ji Bae Youn-Sig Kwak Youn-Sig Kwak Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture Frontiers in Bioengineering and Biotechnology antifungal activity Burkholderia monoculture plant growth promotion Streptomyces turfgrass |
| title | Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture |
| title_full | Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture |
| title_fullStr | Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture |
| title_full_unstemmed | Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture |
| title_short | Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture |
| title_sort | changes in bacterial community structure and enriched functional bacteria associated with turfgrass monoculture |
| topic | antifungal activity Burkholderia monoculture plant growth promotion Streptomyces turfgrass |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2020.530067/full |
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