Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum

Antagonistic activity of two bacteria Bacillus subtilis and Pseudomonas fluorescens against Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus and Fusarium oxysporum of Pennisetum americanum was studied. In vitro studied were carried out in both dual culture technique...

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Main Authors: Gaurav Bhushan, Sweta Chhangani, Jaspal Singh, A.P. Singh
Format: Article
Language:English
Published: Environment, Agriculture and Education Society 2013-12-01
Series:G-Journal of Environmental Science and Technology
Subjects:
Online Access:https://gjestenv.com/index.php/gjest/article/view/17
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author Gaurav Bhushan
Sweta Chhangani
Jaspal Singh
A.P. Singh
author_facet Gaurav Bhushan
Sweta Chhangani
Jaspal Singh
A.P. Singh
author_sort Gaurav Bhushan
collection DOAJ
description Antagonistic activity of two bacteria Bacillus subtilis and Pseudomonas fluorescens against Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus and Fusarium oxysporum of Pennisetum americanum was studied. In vitro studied were carried out in both dual culture technique and blotter test method. In dual culture technique the Bacillus subtilis was most antagonistic ones to the seed-borne mycoflora in vitro, while the other isolate Pseudomonas fluroescens did not show any antagonistic activity on any seed-borne mycoflora. Bacillus subtilis antagonistic isolate as well as the commercial biocide was applied as seed treatment for controlling seed-borne mycoflora under Blotter test in vitro and Pot experiment in vivo conditions. It was observed that maximum seed germination and maximum shoot and root length recorded with Aspergillus flavus and Bacillus subtilis combination in Pot experiment. Experiment shows, that Bacillus subtilis antagonistic isolate was able to significant reduction in seed-borne mycoflora than Pseudomonas fluorescens in Pennisetum americanum.
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spelling doaj.art-280b8eef6ed6436fa7a2b899a9cf03b02023-02-02T04:59:05ZengEnvironment, Agriculture and Education SocietyG-Journal of Environmental Science and Technology2322-02282013-12-0113Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanumGaurav Bhushan0Sweta Chhangani1Jaspal Singh2A.P. Singh3Department of Environmental Science, Bareilly College, Bareilly, U.P. INDIADepartment of Botany, University of Rajasthan, Jaipur, Rajasthan INDIADepartment of Environmental Science, Bareilly College, Bareilly, U.P. INDIADepartment of Environmental Science, Bareilly College, Bareilly, U.P. INDIAAntagonistic activity of two bacteria Bacillus subtilis and Pseudomonas fluorescens against Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus and Fusarium oxysporum of Pennisetum americanum was studied. In vitro studied were carried out in both dual culture technique and blotter test method. In dual culture technique the Bacillus subtilis was most antagonistic ones to the seed-borne mycoflora in vitro, while the other isolate Pseudomonas fluroescens did not show any antagonistic activity on any seed-borne mycoflora. Bacillus subtilis antagonistic isolate as well as the commercial biocide was applied as seed treatment for controlling seed-borne mycoflora under Blotter test in vitro and Pot experiment in vivo conditions. It was observed that maximum seed germination and maximum shoot and root length recorded with Aspergillus flavus and Bacillus subtilis combination in Pot experiment. Experiment shows, that Bacillus subtilis antagonistic isolate was able to significant reduction in seed-borne mycoflora than Pseudomonas fluorescens in Pennisetum americanum.https://gjestenv.com/index.php/gjest/article/view/17Seed-borne mycoflorabiocides agentsBacillus subtilisPseudomonas fluorescensPennisetum americanum seeds
spellingShingle Gaurav Bhushan
Sweta Chhangani
Jaspal Singh
A.P. Singh
Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
G-Journal of Environmental Science and Technology
Seed-borne mycoflora
biocides agents
Bacillus subtilis
Pseudomonas fluorescens
Pennisetum americanum seeds
title Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
title_full Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
title_fullStr Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
title_full_unstemmed Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
title_short Antagonistic Effects of Bacillus subtilis and Pseudomonas fluorescens Against Seed- Borne Mycoflora of Pennisetum americanum
title_sort antagonistic effects of bacillus subtilis and pseudomonas fluorescens against seed borne mycoflora of pennisetum americanum
topic Seed-borne mycoflora
biocides agents
Bacillus subtilis
Pseudomonas fluorescens
Pennisetum americanum seeds
url https://gjestenv.com/index.php/gjest/article/view/17
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