Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice
The anaerobic actinobacterium Gardnerella was first isolated from the bladder by suprapubic aspiration more than 50 years ago. Since then, Gardnerella has been increasingly recognized as a common and often abundant member of the female urinary microbiome (urobiome). Some studies even suggest that th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.909799/full |
| _version_ | 1811246151890894848 |
|---|---|
| author | Nicole M. Gilbert Valerie P. O’Brien Chevaughn Waller Ekatherina Batourina Cathy Lee Mendelsohn Amanda L. Lewis |
| author_facet | Nicole M. Gilbert Valerie P. O’Brien Chevaughn Waller Ekatherina Batourina Cathy Lee Mendelsohn Amanda L. Lewis |
| author_sort | Nicole M. Gilbert |
| collection | DOAJ |
| description | The anaerobic actinobacterium Gardnerella was first isolated from the bladder by suprapubic aspiration more than 50 years ago. Since then, Gardnerella has been increasingly recognized as a common and often abundant member of the female urinary microbiome (urobiome). Some studies even suggest that the presence of Gardnerella is associated with urological disorders in women. We recently reported that inoculation of Gardnerella into the bladders of mice results in urothelial exfoliation. Here, we performed whole bladder RNA-seq in our mouse model to identify additional host pathways involved in the response to Gardnerella bladder exposure. The transcriptional response to Gardnerella reflected the urothelial turnover that is a consequence of exfoliation while also illustrating the activation of pathways involved in inflammation and immunity. Additional timed exposure experiments in mice provided further evidence of a potentially clinically relevant consequence of bladder exposure to Gardnerella—increased susceptibility to subsequent UTI caused by uropathogenic Escherichia coli. Together, these data provide a broader picture of the bladder’s response to Gardnerella and lay the groundwork for future studies examining the impact of Gardnerella on bladder health. |
| first_indexed | 2024-04-12T14:50:29Z |
| format | Article |
| id | doaj.art-280c7a8abf2846e3aacdb0e8e43482d9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T14:50:29Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-280c7a8abf2846e3aacdb0e8e43482d92022-12-22T03:28:29ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.909799909799Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in MiceNicole M. Gilbert0Valerie P. O’Brien1Chevaughn Waller2Ekatherina Batourina3Cathy Lee Mendelsohn4Amanda L. Lewis5Department of Pediatrics, Division of Infectious Diseases, Washington University in St. Louis School of Medicine, St. Louis, MO, United StatesHuman Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United StatesDepartment of Urology, Columbia University Irving Medical Center, New York, NY, United StatesDepartment of Urology, Columbia University Irving Medical Center, New York, NY, United StatesDepartment of Urology, Columbia University Irving Medical Center, New York, NY, United StatesDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Diego, San Diego, CA, United StatesThe anaerobic actinobacterium Gardnerella was first isolated from the bladder by suprapubic aspiration more than 50 years ago. Since then, Gardnerella has been increasingly recognized as a common and often abundant member of the female urinary microbiome (urobiome). Some studies even suggest that the presence of Gardnerella is associated with urological disorders in women. We recently reported that inoculation of Gardnerella into the bladders of mice results in urothelial exfoliation. Here, we performed whole bladder RNA-seq in our mouse model to identify additional host pathways involved in the response to Gardnerella bladder exposure. The transcriptional response to Gardnerella reflected the urothelial turnover that is a consequence of exfoliation while also illustrating the activation of pathways involved in inflammation and immunity. Additional timed exposure experiments in mice provided further evidence of a potentially clinically relevant consequence of bladder exposure to Gardnerella—increased susceptibility to subsequent UTI caused by uropathogenic Escherichia coli. Together, these data provide a broader picture of the bladder’s response to Gardnerella and lay the groundwork for future studies examining the impact of Gardnerella on bladder health.https://www.frontiersin.org/articles/10.3389/fcimb.2022.909799/fullurobiomebladderdysbiosisurotheliumbacterial vaginosisurinary tract infection |
| spellingShingle | Nicole M. Gilbert Valerie P. O’Brien Chevaughn Waller Ekatherina Batourina Cathy Lee Mendelsohn Amanda L. Lewis Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice Frontiers in Cellular and Infection Microbiology urobiome bladder dysbiosis urothelium bacterial vaginosis urinary tract infection |
| title | Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice |
| title_full | Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice |
| title_fullStr | Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice |
| title_full_unstemmed | Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice |
| title_short | Gardnerella Exposures Alter Bladder Gene Expression and Augment Uropathogenic Escherichia coli Urinary Tract Infection in Mice |
| title_sort | gardnerella exposures alter bladder gene expression and augment uropathogenic escherichia coli urinary tract infection in mice |
| topic | urobiome bladder dysbiosis urothelium bacterial vaginosis urinary tract infection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.909799/full |
| work_keys_str_mv | AT nicolemgilbert gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice AT valeriepobrien gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice AT chevaughnwaller gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice AT ekatherinabatourina gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice AT cathyleemendelsohn gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice AT amandallewis gardnerellaexposuresalterbladdergeneexpressionandaugmenturopathogenicescherichiacoliurinarytractinfectioninmice |