The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells
Hydrogel materials have been successfully used as matrices to explore the role of biophysical and biochemical stimuli in directing stem cell behavior. Here, we present our findings on the role of modulus in guiding bone marrow fetal mesenchymal stem cell (BMfMSC) fate determination using semi-synthe...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-12-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01555/full |
| _version_ | 1819053536958218240 |
|---|---|
| author | Abdul Jalil Rufaihah Suganya Cheyyatraivendran Muhammad Danial Mohd Mazlan Kenrich Lim Mark Seow Khoon Chong Citra Nurfarah Zaini Mattar Jerry Kok Yen Chan Theodoros Kofidis Theodoros Kofidis Dror Seliktar Dror Seliktar |
| author_facet | Abdul Jalil Rufaihah Suganya Cheyyatraivendran Muhammad Danial Mohd Mazlan Kenrich Lim Mark Seow Khoon Chong Citra Nurfarah Zaini Mattar Jerry Kok Yen Chan Theodoros Kofidis Theodoros Kofidis Dror Seliktar Dror Seliktar |
| author_sort | Abdul Jalil Rufaihah |
| collection | DOAJ |
| description | Hydrogel materials have been successfully used as matrices to explore the role of biophysical and biochemical stimuli in directing stem cell behavior. Here, we present our findings on the role of modulus in guiding bone marrow fetal mesenchymal stem cell (BMfMSC) fate determination using semi-synthetic hydrogels made from PEG-fibrinogen (PF). The BMfMSCs were cultivated in the PF for up to 2 weeks to study the influence of matrix modulus (i.e., cross-linking density of the PF) on BMfMSC survival, morphology and integrin expression. Both two-dimensional (2D) and three-dimensional (3D) culture conditions were employed to examine the BMfMSCs as single cells or as cell spheroids. The hydrogel modulus affected the rate of BMfMSC metabolic activity, the integrin expression levels and the cell morphology, both as single cells and as spheroids. The cell seeding density was also found to be an important parameter of the system in that high densities were favorable in facilitating more cell-to-cell contacts that favored higher metabolic activity. Our findings provide important insight about design of a hydrogel scaffold that can be used to optimize the biological response of BMfMSCs for various tissue engineering applications. |
| first_indexed | 2024-12-21T12:37:18Z |
| format | Article |
| id | doaj.art-280ec02425e048eda8f8d3e384c7cd3f |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T12:37:18Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-280ec02425e048eda8f8d3e384c7cd3f2022-12-21T19:03:53ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-12-01910.3389/fphys.2018.01555374593The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem CellsAbdul Jalil Rufaihah0Suganya Cheyyatraivendran1Muhammad Danial Mohd Mazlan2Kenrich Lim3Mark Seow Khoon Chong4Citra Nurfarah Zaini Mattar5Jerry Kok Yen Chan6Theodoros Kofidis7Theodoros Kofidis8Dror Seliktar9Dror Seliktar10Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDivision of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, SingaporeDepartment of Obstretics and Gynaecology, National University of Singapore, Singapore, SingaporeDepartment of Obstretics and Gynaecology, National University of Singapore, Singapore, SingaporeDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Cardiac, Thoracic and Vascular Surgery, National University Heart Centre Singapore, National University Health System, Singapore, SingaporeNanoscience and Nanotechnology Initiative, National University of Singapore, Singapore, SingaporeFaculty of Biomedical Engineering, Technion – Israel Institute of Technology, Haifa, IsraelHydrogel materials have been successfully used as matrices to explore the role of biophysical and biochemical stimuli in directing stem cell behavior. Here, we present our findings on the role of modulus in guiding bone marrow fetal mesenchymal stem cell (BMfMSC) fate determination using semi-synthetic hydrogels made from PEG-fibrinogen (PF). The BMfMSCs were cultivated in the PF for up to 2 weeks to study the influence of matrix modulus (i.e., cross-linking density of the PF) on BMfMSC survival, morphology and integrin expression. Both two-dimensional (2D) and three-dimensional (3D) culture conditions were employed to examine the BMfMSCs as single cells or as cell spheroids. The hydrogel modulus affected the rate of BMfMSC metabolic activity, the integrin expression levels and the cell morphology, both as single cells and as spheroids. The cell seeding density was also found to be an important parameter of the system in that high densities were favorable in facilitating more cell-to-cell contacts that favored higher metabolic activity. Our findings provide important insight about design of a hydrogel scaffold that can be used to optimize the biological response of BMfMSCs for various tissue engineering applications.https://www.frontiersin.org/article/10.3389/fphys.2018.01555/fullhydrogelscaffoldtissue engineeringmatrix stiffnessbiomaterialsPEGylated fibrinogen |
| spellingShingle | Abdul Jalil Rufaihah Suganya Cheyyatraivendran Muhammad Danial Mohd Mazlan Kenrich Lim Mark Seow Khoon Chong Citra Nurfarah Zaini Mattar Jerry Kok Yen Chan Theodoros Kofidis Theodoros Kofidis Dror Seliktar Dror Seliktar The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells Frontiers in Physiology hydrogel scaffold tissue engineering matrix stiffness biomaterials PEGylated fibrinogen |
| title | The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells |
| title_full | The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells |
| title_fullStr | The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells |
| title_full_unstemmed | The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells |
| title_short | The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells |
| title_sort | effect of scaffold modulus on the morphology and remodeling of fetal mesenchymal stem cells |
| topic | hydrogel scaffold tissue engineering matrix stiffness biomaterials PEGylated fibrinogen |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01555/full |
| work_keys_str_mv | AT abduljalilrufaihah theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT suganyacheyyatraivendran theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT muhammaddanialmohdmazlan theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT kenrichlim theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT markseowkhoonchong theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT citranurfarahzainimattar theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT jerrykokyenchan theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT theodoroskofidis theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT theodoroskofidis theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT drorseliktar theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT drorseliktar theeffectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT abduljalilrufaihah effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT suganyacheyyatraivendran effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT muhammaddanialmohdmazlan effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT kenrichlim effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT markseowkhoonchong effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT citranurfarahzainimattar effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT jerrykokyenchan effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT theodoroskofidis effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT theodoroskofidis effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT drorseliktar effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells AT drorseliktar effectofscaffoldmodulusonthemorphologyandremodelingoffetalmesenchymalstemcells |