Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
Abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-021-00184-6 |
| _version_ | 1797350045104734208 |
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| author | Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira |
| author_facet | Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira |
| author_sort | Bárbara M. de Sousa |
| collection | DOAJ |
| description | Abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial osseointegration but lack long-term personalized actuation on the bone–implant interface. Novel bioelectronic devices comprising biophysical stimulators and sensing systems are thus emerging, aiming for long-term control of peri-implant bone growth through biointerface monitoring. These acting-sensing dual systems require high frequency (HF) operations able to stimulate osteoinduction/osteoconduction, including matrix maturation and mineralization. A sensing-compatible capacitive stimulator of thin interdigitated electrodes and delivering an electrical 60 kHz HF stimulation, 30 min/day, is here shown to promote osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). HF stimulation through this capacitive interdigitated system had significant effects on osteoblasts’ collagen-I synthesis, matrix, and mineral deposition. A proteomic analysis of microvesicles released from electrically-stimulated osteoblasts revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). Proteomics data are available via ProteomeXchange with the identifier PXD028551. Further, under HF stimulation, hASCs exhibited higher osteogenic commitment and enhanced hydroxyapatite deposition. These promising osteoinductive/conductive capacitive stimulators will integrate novel bioelectronic implants able to monitor the bone–implant interface and deliver personalized stimulation to peri-implant tissues. |
| first_indexed | 2024-03-08T12:39:09Z |
| format | Article |
| id | doaj.art-281173fd7b014b119611c44e55c55dd1 |
| institution | Directory Open Access Journal |
| issn | 2057-3995 |
| language | English |
| last_indexed | 2024-03-08T12:39:09Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj.art-281173fd7b014b119611c44e55c55dd12024-01-21T12:13:20ZengNature Portfolionpj Regenerative Medicine2057-39952021-11-016111410.1038/s41536-021-00184-6Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implantsBárbara M. de Sousa0Clara R. Correia1Jorge A. F. Ferreira2João F. Mano3Edward P. Furlani4Marco P. Soares dos Santos5Sandra I. Vieira6Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of AveiroDepartment of Chemistry, CICECO – Aveiro Institute of Materials, University of AveiroDepartment of Mechanical Engineering, Centre for Mechanical Technology & Automation (TEMA), University of AveiroDepartment of Chemistry, CICECO – Aveiro Institute of Materials, University of AveiroDepartment of Chemical and Biological Engineering, Department of Electrical Engineering, University at Buffalo (SUNY)Department of Mechanical Engineering, Centre for Mechanical Technology & Automation (TEMA), University of AveiroDepartment of Medical Sciences, Institute of Biomedicine (iBiMED), University of AveiroAbstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial osseointegration but lack long-term personalized actuation on the bone–implant interface. Novel bioelectronic devices comprising biophysical stimulators and sensing systems are thus emerging, aiming for long-term control of peri-implant bone growth through biointerface monitoring. These acting-sensing dual systems require high frequency (HF) operations able to stimulate osteoinduction/osteoconduction, including matrix maturation and mineralization. A sensing-compatible capacitive stimulator of thin interdigitated electrodes and delivering an electrical 60 kHz HF stimulation, 30 min/day, is here shown to promote osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). HF stimulation through this capacitive interdigitated system had significant effects on osteoblasts’ collagen-I synthesis, matrix, and mineral deposition. A proteomic analysis of microvesicles released from electrically-stimulated osteoblasts revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). Proteomics data are available via ProteomeXchange with the identifier PXD028551. Further, under HF stimulation, hASCs exhibited higher osteogenic commitment and enhanced hydroxyapatite deposition. These promising osteoinductive/conductive capacitive stimulators will integrate novel bioelectronic implants able to monitor the bone–implant interface and deliver personalized stimulation to peri-implant tissues.https://doi.org/10.1038/s41536-021-00184-6 |
| spellingShingle | Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants npj Regenerative Medicine |
| title | Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants |
| title_full | Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants |
| title_fullStr | Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants |
| title_full_unstemmed | Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants |
| title_short | Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants |
| title_sort | capacitive interdigitated system of high osteoinductive conductive performance for personalized acting sensing implants |
| url | https://doi.org/10.1038/s41536-021-00184-6 |
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