The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection
To successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1140101/full |
| _version_ | 1797859201206190080 |
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| author | Andrei Kiselev Laurent Camborde Laura Ossorio Carballo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Elodie Gaulin |
| author_facet | Andrei Kiselev Laurent Camborde Laura Ossorio Carballo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Elodie Gaulin |
| author_sort | Andrei Kiselev |
| collection | DOAJ |
| description | To successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we aimed to identify active secreted proteases from the oomycete Aphanomyces euteiches, which causes root rot diseases on legumes. Genome mining and expression analysis highlighted an overrepresentation of microbial tandemly repeated proteases, which are upregulated during host infection. Activity Based Protein Profiling and mass spectrometry (ABPP-MS) on apoplastic fluids isolated from pea roots infected by the pathogen led to the identification of 35 active extracellular microbial proteases, which represents around 30% of the genes expressed encoding serine and cysteine proteases during infection. Notably, eight of the detected active secreted proteases carry an additional C-terminal domain. This study reveals novel active modular extracellular eukaryotic proteases as potential pathogenicity factors in Aphanomyces genus. |
| first_indexed | 2024-04-09T21:25:37Z |
| format | Article |
| id | doaj.art-2813ac0ce7d549ac9d42df06c7c0cc34 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T21:25:37Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2813ac0ce7d549ac9d42df06c7c0cc342023-03-27T14:08:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11401011140101The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infectionAndrei Kiselev0Laurent Camborde1Laura Ossorio Carballo2Farnusch Kaschani3Markus Kaiser4Renier A. L. van der Hoorn5Elodie Gaulin6Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, Auzeville-Tolosane, FranceLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, Auzeville-Tolosane, FranceThe Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, Oxford, United KingdomZMB Chemical Biology, Faculty of Biology, University of Duisburg-Essen, Essen, GermanyZMB Chemical Biology, Faculty of Biology, University of Duisburg-Essen, Essen, GermanyThe Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, Oxford, United KingdomLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, Auzeville-Tolosane, FranceTo successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we aimed to identify active secreted proteases from the oomycete Aphanomyces euteiches, which causes root rot diseases on legumes. Genome mining and expression analysis highlighted an overrepresentation of microbial tandemly repeated proteases, which are upregulated during host infection. Activity Based Protein Profiling and mass spectrometry (ABPP-MS) on apoplastic fluids isolated from pea roots infected by the pathogen led to the identification of 35 active extracellular microbial proteases, which represents around 30% of the genes expressed encoding serine and cysteine proteases during infection. Notably, eight of the detected active secreted proteases carry an additional C-terminal domain. This study reveals novel active modular extracellular eukaryotic proteases as potential pathogenicity factors in Aphanomyces genus.https://www.frontiersin.org/articles/10.3389/fpls.2023.1140101/fullAphanomycesroot rotplant pathogenproteasesapoplastextracellular |
| spellingShingle | Andrei Kiselev Laurent Camborde Laura Ossorio Carballo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Elodie Gaulin The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection Frontiers in Plant Science Aphanomyces root rot plant pathogen proteases apoplast extracellular |
| title | The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| title_full | The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| title_fullStr | The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| title_full_unstemmed | The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| title_short | The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| title_sort | root pathogen aphanomyces euteiches secretes modular proteases in pea apoplast during host infection |
| topic | Aphanomyces root rot plant pathogen proteases apoplast extracellular |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1140101/full |
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