Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice
Abstract N6-methyladenosine (m6A) modification has been shown to regulate RNA metabolism. Here, we investigate m6A dynamics during maternal-to-zygotic transition (MZT) in mice through multi-omic analysis. Our results show that m6A can be maternally inherited or de novo gained after fertilization. In...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Genome Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13059-023-02918-9 |
| _version_ | 1797850017687404544 |
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| author | Wencheng Zhu Yufeng Ding Juan Meng Lei Gu Wenjun Liu Li Li Hongyu Chen Yining Wang Ziyi Li Chen Li Yidi Sun Zhen Liu |
| author_facet | Wencheng Zhu Yufeng Ding Juan Meng Lei Gu Wenjun Liu Li Li Hongyu Chen Yining Wang Ziyi Li Chen Li Yidi Sun Zhen Liu |
| author_sort | Wencheng Zhu |
| collection | DOAJ |
| description | Abstract N6-methyladenosine (m6A) modification has been shown to regulate RNA metabolism. Here, we investigate m6A dynamics during maternal-to-zygotic transition (MZT) in mice through multi-omic analysis. Our results show that m6A can be maternally inherited or de novo gained after fertilization. Interestingly, m6A modification on maternal mRNAs not only correlates with mRNA degradation, but also maintains the stability of a small group of mRNAs thereby promoting their translation after fertilization. We identify Ythdc1 and Ythdf2 as key m6A readers for mouse preimplantation development. Our study reveals a key role of m6A mediated RNA metabolism during MZT in mammals. |
| first_indexed | 2024-04-09T18:53:34Z |
| format | Article |
| id | doaj.art-2815cc0862a749eebd1438a1ac5ce677 |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-04-09T18:53:34Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-2815cc0862a749eebd1438a1ac5ce6772023-04-09T11:17:45ZengBMCGenome Biology1474-760X2023-04-0124111610.1186/s13059-023-02918-9Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in miceWencheng Zhu0Yufeng Ding1Juan Meng2Lei Gu3Wenjun Liu4Li Li5Hongyu Chen6Yining Wang7Ziyi Li8Chen Li9Yidi Sun10Zhen Liu11Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of MedicineInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesShanghai Applied Protein Technology Co., Ltd.Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of MedicineInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of SciencesAbstract N6-methyladenosine (m6A) modification has been shown to regulate RNA metabolism. Here, we investigate m6A dynamics during maternal-to-zygotic transition (MZT) in mice through multi-omic analysis. Our results show that m6A can be maternally inherited or de novo gained after fertilization. Interestingly, m6A modification on maternal mRNAs not only correlates with mRNA degradation, but also maintains the stability of a small group of mRNAs thereby promoting their translation after fertilization. We identify Ythdc1 and Ythdf2 as key m6A readers for mouse preimplantation development. Our study reveals a key role of m6A mediated RNA metabolism during MZT in mammals.https://doi.org/10.1186/s13059-023-02918-9m6A landscapeMulti-omicsMaternal-to-zygotic transitionYthdc1Ythdf2 |
| spellingShingle | Wencheng Zhu Yufeng Ding Juan Meng Lei Gu Wenjun Liu Li Li Hongyu Chen Yining Wang Ziyi Li Chen Li Yidi Sun Zhen Liu Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice Genome Biology m6A landscape Multi-omics Maternal-to-zygotic transition Ythdc1 Ythdf2 |
| title | Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice |
| title_full | Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice |
| title_fullStr | Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice |
| title_full_unstemmed | Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice |
| title_short | Reading and writing of mRNA m6A modification orchestrate maternal-to-zygotic transition in mice |
| title_sort | reading and writing of mrna m6a modification orchestrate maternal to zygotic transition in mice |
| topic | m6A landscape Multi-omics Maternal-to-zygotic transition Ythdc1 Ythdf2 |
| url | https://doi.org/10.1186/s13059-023-02918-9 |
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