Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey.
The cyclic peptide Melanin-Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory and reward. Based on neuroanatomical and electrophysiological studies these functions were at...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-11-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00182/full |
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| author | Gregory eConductier Gregory eConductier Agnès eMartin Agnès eMartin Agnès eMartin Pierre-Yves eRisold Sonia eJégo Raphaël eLavoie Chrystel eLafont Chrystel eLafont Chrystel eLafont Patrice eMollard Patrice eMollard Patrice eMollard Antoine eAdamantidis Jean-Louis eNahon Jean-Louis eNahon Jean-Louis eNahon |
| author_facet | Gregory eConductier Gregory eConductier Agnès eMartin Agnès eMartin Agnès eMartin Pierre-Yves eRisold Sonia eJégo Raphaël eLavoie Chrystel eLafont Chrystel eLafont Chrystel eLafont Patrice eMollard Patrice eMollard Patrice eMollard Antoine eAdamantidis Jean-Louis eNahon Jean-Louis eNahon Jean-Louis eNahon |
| author_sort | Gregory eConductier |
| collection | DOAJ |
| description | The cyclic peptide Melanin-Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory and reward. Based on neuroanatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (Conductier et al. 2013) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1 knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis. |
| first_indexed | 2024-12-14T01:10:09Z |
| format | Article |
| id | doaj.art-28173dd2c069486992dc8b03328b1b02 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-14T01:10:09Z |
| publishDate | 2013-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-28173dd2c069486992dc8b03328b1b022022-12-21T23:22:47ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922013-11-01410.3389/fendo.2013.0018265503Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey.Gregory eConductier0Gregory eConductier1Agnès eMartin2Agnès eMartin3Agnès eMartin4Pierre-Yves eRisold5Sonia eJégo6Raphaël eLavoie7Chrystel eLafont8Chrystel eLafont9Chrystel eLafont10Patrice eMollard11Patrice eMollard12Patrice eMollard13Antoine eAdamantidis14Jean-Louis eNahon15Jean-Louis eNahon16Jean-Louis eNahon17Institut de Pharmacologie Moleculaire et Cellulaire, UMR CNRS 7275University of Nice Sophia AntipolisInstitut de Genomique Fonctionnelle, CNRS, UMR-5203, Institut de Génomique Fonctionnelle; CNRS UMR-5203; INSERM, U661; Universités de Montpellier 1 & 2INSERM U661Universités de Montpellier 1 and 2 UMR 5203IFR 133, faculté de médecine et de pharmacieDouglass Mental Health University IntituteDouglass Mental Health University IntituteInstitut de Genomique Fonctionnelle, CNRS, UMR-5203, Institut de Génomique Fonctionnelle; CNRS UMR-5203; INSERM, U661; Universités de Montpellier 1 & 2INSERM U661Universités de Montpellier 1 and 2 UMR 5203Institut de Genomique Fonctionnelle, CNRS, UMR-5203, Institut de Génomique Fonctionnelle; CNRS UMR-5203; INSERM, U661; Universités de Montpellier 1 & 2INSERM U661Universités de Montpellier 1 and 2 UMR 5203Douglass Mental Health University IntituteInstitut de Pharmacologie Moleculaire et Cellulaire, UMR CNRS 7275University of Nice Sophia AntipolisStation de Primatologie UPS 846 CNRSThe cyclic peptide Melanin-Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory and reward. Based on neuroanatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (Conductier et al. 2013) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1 knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00182/fullCerebrospinal FluidCiliaMCHMCHR1cerebraospinal fluid flownon neuronal function |
| spellingShingle | Gregory eConductier Gregory eConductier Agnès eMartin Agnès eMartin Agnès eMartin Pierre-Yves eRisold Sonia eJégo Raphaël eLavoie Chrystel eLafont Chrystel eLafont Chrystel eLafont Patrice eMollard Patrice eMollard Patrice eMollard Antoine eAdamantidis Jean-Louis eNahon Jean-Louis eNahon Jean-Louis eNahon Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. Frontiers in Endocrinology Cerebrospinal Fluid Cilia MCH MCHR1 cerebraospinal fluid flow non neuronal function |
| title | Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. |
| title_full | Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. |
| title_fullStr | Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. |
| title_full_unstemmed | Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. |
| title_short | Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey. |
| title_sort | control of ventricular ciliary beating by the melanin concentrating hormone expressing neurons of the lateral hypothalamus a functional imaging survey |
| topic | Cerebrospinal Fluid Cilia MCH MCHR1 cerebraospinal fluid flow non neuronal function |
| url | http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00182/full |
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