Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51
Brucella abortus is a gram negative, zoonotic pathogen that can cause abortions and stillbirths in the cattle industry and has contributed to significant economic losses to cow-calf producers. Cell mediated immunity (CMI) is an important component of the immune response associated with protection ag...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1105485/full |
| _version_ | 1797904896654049280 |
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| author | Lauren Crawford Lauren Crawford Lauren Crawford Shollie Falkenberg Ellie Jordan Putz Steven Olsen Paola M. Boggiatto |
| author_facet | Lauren Crawford Lauren Crawford Lauren Crawford Shollie Falkenberg Ellie Jordan Putz Steven Olsen Paola M. Boggiatto |
| author_sort | Lauren Crawford |
| collection | DOAJ |
| description | Brucella abortus is a gram negative, zoonotic pathogen that can cause abortions and stillbirths in the cattle industry and has contributed to significant economic losses to cow-calf producers. Cell mediated immunity (CMI) is an important component of the immune response associated with protection against Brucella abortus and other intracellular pathogens. Brucellosis and viral modified live vaccines (vMLV) are licensed individually but may be used concurrently under field conditions. Peripheral blood mononuclear cells (PBMC) from non-vaccinated cattle and cattle vaccinated with either Brucella abortus strain RB51, a vMLV or both RB51 and a vMLV vaccine were isolated. The frequency of CD4+, CD8+ and γδ+ T cell populations within PBMC, and the frequency of interferon gamma (IFN-γ) production within these cell types was characterized via flow-cytometry. The goal of this study was to characterize immune responses to RB51 vaccination and determine the effect of concurrent vaccine administration. Although immune responses were greatest in PBMC from cattle vaccinated with only RB51, cattle vaccinated with both RB51 and vMLV demonstrated measurable T cell responses associated with protective immunity. Data suggests a lack of significant biological differences between the groups in protective immune responses. Collectively, our data demonstrated a lack of vaccine interference following concurrent administration of vMLV and RB51. Although concurrent administration of individually licensed vaccines may influence immune responses and contribute to vaccine interference, potential vaccine combinations should be evaluated for biological effects. |
| first_indexed | 2024-04-10T09:56:18Z |
| format | Article |
| id | doaj.art-281bf87512254c7b9e4cefec8f057e8c |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T09:56:18Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-281bf87512254c7b9e4cefec8f057e8c2023-02-16T12:18:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-02-011010.3389/fvets.2023.11054851105485Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51Lauren Crawford0Lauren Crawford1Lauren Crawford2Shollie Falkenberg3Ellie Jordan Putz4Steven Olsen5Paola M. Boggiatto6Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Ames, IA, United StatesORISE, Oak Ridge, TN, United StatesImmunobiology Graduate Program, Iowa State University, Ames, IA, United StatesDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesInfectious Bacterial Diseases Research Unit, National Animal Disease Center, Ames, IA, United StatesInfectious Bacterial Diseases Research Unit, National Animal Disease Center, Ames, IA, United StatesInfectious Bacterial Diseases Research Unit, National Animal Disease Center, Ames, IA, United StatesBrucella abortus is a gram negative, zoonotic pathogen that can cause abortions and stillbirths in the cattle industry and has contributed to significant economic losses to cow-calf producers. Cell mediated immunity (CMI) is an important component of the immune response associated with protection against Brucella abortus and other intracellular pathogens. Brucellosis and viral modified live vaccines (vMLV) are licensed individually but may be used concurrently under field conditions. Peripheral blood mononuclear cells (PBMC) from non-vaccinated cattle and cattle vaccinated with either Brucella abortus strain RB51, a vMLV or both RB51 and a vMLV vaccine were isolated. The frequency of CD4+, CD8+ and γδ+ T cell populations within PBMC, and the frequency of interferon gamma (IFN-γ) production within these cell types was characterized via flow-cytometry. The goal of this study was to characterize immune responses to RB51 vaccination and determine the effect of concurrent vaccine administration. Although immune responses were greatest in PBMC from cattle vaccinated with only RB51, cattle vaccinated with both RB51 and vMLV demonstrated measurable T cell responses associated with protective immunity. Data suggests a lack of significant biological differences between the groups in protective immune responses. Collectively, our data demonstrated a lack of vaccine interference following concurrent administration of vMLV and RB51. Although concurrent administration of individually licensed vaccines may influence immune responses and contribute to vaccine interference, potential vaccine combinations should be evaluated for biological effects.https://www.frontiersin.org/articles/10.3389/fvets.2023.1105485/fullruminant immunologylivestock vaccinationT helper 1 (IFN-γ)modified live vaccine (MLV)cell mediated immunity (CMI) |
| spellingShingle | Lauren Crawford Lauren Crawford Lauren Crawford Shollie Falkenberg Ellie Jordan Putz Steven Olsen Paola M. Boggiatto Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 Frontiers in Veterinary Science ruminant immunology livestock vaccination T helper 1 (IFN-γ) modified live vaccine (MLV) cell mediated immunity (CMI) |
| title | Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 |
| title_full | Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 |
| title_fullStr | Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 |
| title_full_unstemmed | Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 |
| title_short | Effects of concurrent administration of modified live viral vaccines with RB51 on immune responses to RB51 |
| title_sort | effects of concurrent administration of modified live viral vaccines with rb51 on immune responses to rb51 |
| topic | ruminant immunology livestock vaccination T helper 1 (IFN-γ) modified live vaccine (MLV) cell mediated immunity (CMI) |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1105485/full |
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