Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination
ABSTRACT Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm), usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the...
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| Language: | English |
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Sociedade Botânica do Brasil
2016-01-01
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| Series: | Acta Botânica Brasílica |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016005007103&lng=en&tlng=en |
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| author | Elisa Monteze Bicalho Sérgio Yoshimitsu Motoike Eduardo Euclydes de Lima e Borges Glauciana da Mata Ataíde Valéria Monteze Guimarães |
| author_facet | Elisa Monteze Bicalho Sérgio Yoshimitsu Motoike Eduardo Euclydes de Lima e Borges Glauciana da Mata Ataíde Valéria Monteze Guimarães |
| author_sort | Elisa Monteze Bicalho |
| collection | DOAJ |
| description | ABSTRACT Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm), usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the composition of storage compounds in macaw palm seed and the roles of two enzymes (endo-β-mannanase, α-galactosidase) during and after germination. The seeds were firstly submitted to pre-established protocol to overcome dormancy and promote germination. Enzyme activity in both embryo and endosperm were assayed from the initiation of germinative activities until leaf sheath appearance, and the status of seed structures and reserve compounds were evaluated. Protein content of the embryo decreased with the initiation of imbibition while the lipid content began decreasing six days after removal of the operculum. Increases in enzyme activity and starch content were both observed after visible germination. We suggest that endo-β-mannanase and α-galactosidase become active immediately at germination, facilitating haustorium expansion and providing carbohydrates for initial seedling development. Protein is the first storage compound mobilized during early imbibition, and the observed increase in the starch content of the haustorium was related to lipid degradation in that organ and mannan degradation in the adjacent endosperm. |
| first_indexed | 2024-12-20T09:53:30Z |
| format | Article |
| id | doaj.art-281ea8ee85e647cf8614e3e4d076eb3f |
| institution | Directory Open Access Journal |
| issn | 1677-941X |
| language | English |
| last_indexed | 2024-12-20T09:53:30Z |
| publishDate | 2016-01-01 |
| publisher | Sociedade Botânica do Brasil |
| record_format | Article |
| series | Acta Botânica Brasílica |
| spelling | doaj.art-281ea8ee85e647cf8614e3e4d076eb3f2022-12-21T19:44:31ZengSociedade Botânica do BrasilActa Botânica Brasílica1677-941X2016-01-01010.1590/0102-33062016abb0181S0102-33062016005007103Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germinationElisa Monteze BicalhoSérgio Yoshimitsu MotoikeEduardo Euclydes de Lima e BorgesGlauciana da Mata AtaídeValéria Monteze GuimarãesABSTRACT Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm), usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the composition of storage compounds in macaw palm seed and the roles of two enzymes (endo-β-mannanase, α-galactosidase) during and after germination. The seeds were firstly submitted to pre-established protocol to overcome dormancy and promote germination. Enzyme activity in both embryo and endosperm were assayed from the initiation of germinative activities until leaf sheath appearance, and the status of seed structures and reserve compounds were evaluated. Protein content of the embryo decreased with the initiation of imbibition while the lipid content began decreasing six days after removal of the operculum. Increases in enzyme activity and starch content were both observed after visible germination. We suggest that endo-β-mannanase and α-galactosidase become active immediately at germination, facilitating haustorium expansion and providing carbohydrates for initial seedling development. Protein is the first storage compound mobilized during early imbibition, and the observed increase in the starch content of the haustorium was related to lipid degradation in that organ and mannan degradation in the adjacent endosperm.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016005007103&lng=en&tlng=enα-galactosidaseArecaceaeendo-β-mannanasegalactomannanlipidpalm seedproteinstarch |
| spellingShingle | Elisa Monteze Bicalho Sérgio Yoshimitsu Motoike Eduardo Euclydes de Lima e Borges Glauciana da Mata Ataíde Valéria Monteze Guimarães Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination Acta Botânica Brasílica α-galactosidase Arecaceae endo-β-mannanase galactomannan lipid palm seed protein starch |
| title | Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination |
| title_full | Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination |
| title_fullStr | Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination |
| title_full_unstemmed | Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination |
| title_short | Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination |
| title_sort | enzyme activity and reserve mobilization during macaw palm acrocomia aculeata seed germination |
| topic | α-galactosidase Arecaceae endo-β-mannanase galactomannan lipid palm seed protein starch |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016005007103&lng=en&tlng=en |
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