Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus

In recent years, African swine fever (ASF) has caused a devastating blow to the swine industry globally. Since no effective vaccine is available, strict biosafety measures and rapid diagnosis are the most effective strategies for ASF control. ASFV p30 is one of the most antigenic viral proteins that...

Full description

Bibliographic Details
Main Authors:	Jingjing Ding, Jifei Yang, Daoyuan Jiang, Yanyang Zhou, Chenxi Li, Yanhua Li
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-09-01
Series:	Frontiers in Cellular and Infection Microbiology
Subjects:	African swine fever virus luciferase immunoprecipitation system gaussia luciferase p30 p54 Serological diagnosis
Online Access:	https://www.frontiersin.org/articles/10.3389/fcimb.2022.988355/full

Similar Items

A Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing a Gaussia Luciferase for Antiviral Drug Screening Assay and Luciferase-Based Neutralization Assay
by: Yanhua Li, et al.
Published: (2022-05-01)

A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase
by: Ping Li, et al.
Published: (2018-06-01)

A Recombinant Genotype I Japanese Encephalitis Virus Expressing a <i>Gaussia</i> Luciferase Gene for Antiviral Drug Screening Assay and Neutralizing Antibodies Detection
by: Chenxi Li, et al.
Published: (2022-12-01)

Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus
by: Jie Li, et al.
Published: (2018-11-01)

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes
by: Casey A Maguire, et al.
Published: (2013-01-01)

Gaussia Luciferase as a Reporter for Quorum Sensing in <i>Staphylococcus aureus</i>
by: Isobel Blower, et al.
Published: (2020-08-01)

Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression
by: Michael Puckette, et al.
Published: (2017-06-01)

Seroprevalence of toxoplasmosis among reproductive-aged women in Myanmar and evaluation of luciferase immunoprecipitation system assay
by: Khin Myo Aye, et al.
Published: (2020-11-01)

An improved luciferase immunosorbent assay for ultrasensitive detection of antibodies against African swine fever virus
by: Qiongjie Wang, et al.
Published: (2022-09-01)

Evaluation of Antibody Response Directed against Porcine Reproductive and Respiratory Syndrome Virus Structural Proteins
by: Hung Q. Luong, et al.
Published: (2020-09-01)

Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means
by: Michael Puckette, et al.
Published: (2022-03-01)

Advancing Luciferase-Based Antibody Immunoassays to Next-Generation Mix and Read Testing
by: Peter D. Burbelo, et al.
Published: (2023-02-01)

Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines
by: Hung Q. Luong, et al.
Published: (2023-11-01)

Screening and identification of the dominant antigens of the African swine fever virus
by: Zhaoyang Xu, et al.
Published: (2023-05-01)

Localization of the Catalytic Domain of Copepod Luciferases: Analysis of Truncated Mutants of the <i>Metridia longa</i> Luciferase
by: Svetlana V. Markova, et al.
Published: (2023-05-01)

Triple Reporter Assay: A Non-Overlapping Luciferase Assay for the Measurement of Complex Macromolecular Regulation in Cancer Cells Using a New Mushroom Luciferase–Luciferin Pair
by: Aaiyas Mujawar, et al.
Published: (2023-08-01)

Basic characterization of antibodies targeting receptors of the tumor necrosis factor receptor superfamily
by: Olena Zaitseva, et al.
Published: (2023-03-01)

Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays
by: Sung-Bae Kim, et al.
Published: (2023-07-01)

Processing and Subcellular Localization of the Hepatitis E Virus Replicase: Identification of Candidate Viral Factories
by: Karoline Metzger, et al.
Published: (2022-02-01)

Is LRP2 Involved in Leptin Transport over the Blood-Brain Barrier and Development of Obesity?
by: Elvira S. Sandin, et al.
Published: (2021-05-01)

Identification of Vaccinia Virus Inhibitors and Cellular Functions Necessary for Efficient Viral Replication by Screening Bioactives and FDA-Approved Drugs
by: Chen Peng, et al.
Published: (2020-07-01)

Constitutive glucose dehydrogenase elevates intracellular NADPH levels and luciferase luminescence in Bacillus subtilis
by: Yuzheng Wu, et al.
Published: (2022-12-01)

Novel p22 and p30 dual-proteins combination based indirect ELISA for detecting antibodies against African swine fever virus
by: Jianda Li, et al.
Published: (2023-02-01)

Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma
by: Peter D. Burbelo, et al.
Published: (2019-08-01)

Assessment of the Anti-Amyloidogenic Properties of Essential Oils and Their Constituents in Cells Using a Whole-Cell Recombinant Biosensor
by: Electra Stylianopoulou, et al.
Published: (2023-12-01)

HSV as A Platform for the Generation of Retargeted, Armed, and Reporter-Expressing Oncolytic Viruses
by: Laura Menotti, et al.
Published: (2018-06-01)

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase
by: Duc Long Nguyen, et al.
Published: (2018-03-01)

Evaluation of Firefly and <i>Renilla</i> Luciferase Inhibition in Reporter-Gene Assays: A Case of Isoflavonoids
by: Maša Kenda, et al.
Published: (2021-06-01)

Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens
by: Michael Tessler, et al.
Published: (2018-09-01)

Indirect ELISA Using Multi–Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs
by: Dexin Li, et al.
Published: (2022-11-01)

β‐arrestin2 recruitment at the β2 adrenergic receptor: A luciferase complementation assay adapted for undergraduate training in pharmacology
by: Mattia Ferraiolo, et al.
Published: (2021-02-01)

A Dual Luciferase Reporter System for B. burgdorferi Measures Transcriptional Activity during Tick-Pathogen Interactions
by: Philip P. Adams, et al.
Published: (2017-05-01)

Robust Normalization of Luciferase Reporter Data
by: Andrea Repele, et al.
Published: (2019-07-01)

Approaches to engineer stability of beetle luciferases
by: Mikhail Koksharov, et al.
Published: (2012-09-01)

Firefly Algorithm With Luciferase Inhibition Mechanism
by: Hu Peng, et al.
Published: (2019-01-01)

Serological Detection of SARS-CoV-2 Antibodies in Naturally-Infected Mink and Other Experimentally-Infected Animals
by: Francisco J. Berguido, et al.
Published: (2021-08-01)

Absence of SARS-CoV-2 infection in cats and dogs in close contact with a cluster of COVID-19 patients in a veterinary campus
by: Sarah Temmam, et al.
Published: (2020-12-01)

Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay
by: Aleš Berlec, et al.
Published: (2021-02-01)

Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging
by: A. Chini, et al.
Published: (2002-12-01)

Identification of the Offspring of Vegfr2-luc Transgenic Mouse
by: Qinghua ZHOU, et al.
Published: (2011-05-01)