Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach

Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach

Key points We demonstrate that an unmodified viromic next-generation sequencing methodology effectively detects bacterial pathogen ribosomal RNA in cerebrospinal fluid samples. Sensitivity and specificity displayed pathogen dependent variation. Our finding in principle offers avenues for simultaneou...

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Bibliographic Details
Main Authors: Cormac M. Kinsella, Arthur W. D. Edridge, Ingeborg E. van Zeggeren, Martin Deijs, Diederik van de Beek, Matthijs C. Brouwer, Lia van der Hoek
Format: Article
Language:English
Published: BMC 2022-12-01
Series:Fluids and Barriers of the CNS
Subjects:
Bacterial meningitis
Pathogen detection
Cerebrospinal fluid
Viromics
Metagenomics
Online Access:https://doi.org/10.1186/s12987-022-00400-5
Description
Summary:Key points We demonstrate that an unmodified viromic next-generation sequencing methodology effectively detects bacterial pathogen ribosomal RNA in cerebrospinal fluid samples. Sensitivity and specificity displayed pathogen dependent variation. Our finding in principle offers avenues for simultaneous detection of neuropathogenic viruses and bacteria.
ISSN:2045-8118

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