Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach
Key points We demonstrate that an unmodified viromic next-generation sequencing methodology effectively detects bacterial pathogen ribosomal RNA in cerebrospinal fluid samples. Sensitivity and specificity displayed pathogen dependent variation. Our finding in principle offers avenues for simultaneou...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | Fluids and Barriers of the CNS |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12987-022-00400-5 |
| _version_ | 1797977244180676608 |
|---|---|
| author | Cormac M. Kinsella Arthur W. D. Edridge Ingeborg E. van Zeggeren Martin Deijs Diederik van de Beek Matthijs C. Brouwer Lia van der Hoek |
| author_facet | Cormac M. Kinsella Arthur W. D. Edridge Ingeborg E. van Zeggeren Martin Deijs Diederik van de Beek Matthijs C. Brouwer Lia van der Hoek |
| author_sort | Cormac M. Kinsella |
| collection | DOAJ |
| description | Key points We demonstrate that an unmodified viromic next-generation sequencing methodology effectively detects bacterial pathogen ribosomal RNA in cerebrospinal fluid samples. Sensitivity and specificity displayed pathogen dependent variation. Our finding in principle offers avenues for simultaneous detection of neuropathogenic viruses and bacteria. |
| first_indexed | 2024-04-11T05:04:59Z |
| format | Article |
| id | doaj.art-28229ec4574642eda9dc038732c4016e |
| institution | Directory Open Access Journal |
| issn | 2045-8118 |
| language | English |
| last_indexed | 2024-04-11T05:04:59Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Fluids and Barriers of the CNS |
| spelling | doaj.art-28229ec4574642eda9dc038732c4016e2022-12-25T12:26:16ZengBMCFluids and Barriers of the CNS2045-81182022-12-011911910.1186/s12987-022-00400-5Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approachCormac M. Kinsella0Arthur W. D. Edridge1Ingeborg E. van Zeggeren2Martin Deijs3Diederik van de Beek4Matthijs C. Brouwer5Lia van der Hoek6Amsterdam UMC, Laboratory of Experimental Virology, Department of Medical Microbiology and Infection Prevention, University of AmsterdamAmsterdam UMC, Laboratory of Experimental Virology, Department of Medical Microbiology and Infection Prevention, University of AmsterdamAmsterdam UMC, Department of Neurology, University of AmsterdamAmsterdam UMC, Laboratory of Experimental Virology, Department of Medical Microbiology and Infection Prevention, University of AmsterdamAmsterdam UMC, Department of Neurology, University of AmsterdamAmsterdam UMC, Department of Neurology, University of AmsterdamAmsterdam UMC, Laboratory of Experimental Virology, Department of Medical Microbiology and Infection Prevention, University of AmsterdamKey points We demonstrate that an unmodified viromic next-generation sequencing methodology effectively detects bacterial pathogen ribosomal RNA in cerebrospinal fluid samples. Sensitivity and specificity displayed pathogen dependent variation. Our finding in principle offers avenues for simultaneous detection of neuropathogenic viruses and bacteria.https://doi.org/10.1186/s12987-022-00400-5Bacterial meningitisPathogen detectionCerebrospinal fluidViromicsMetagenomics |
| spellingShingle | Cormac M. Kinsella Arthur W. D. Edridge Ingeborg E. van Zeggeren Martin Deijs Diederik van de Beek Matthijs C. Brouwer Lia van der Hoek Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach Fluids and Barriers of the CNS Bacterial meningitis Pathogen detection Cerebrospinal fluid Viromics Metagenomics |
| title | Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach |
| title_full | Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach |
| title_fullStr | Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach |
| title_full_unstemmed | Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach |
| title_short | Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach |
| title_sort | bacterial ribosomal rna detection in cerebrospinal fluid using a viromics approach |
| topic | Bacterial meningitis Pathogen detection Cerebrospinal fluid Viromics Metagenomics |
| url | https://doi.org/10.1186/s12987-022-00400-5 |
| work_keys_str_mv | AT cormacmkinsella bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT arthurwdedridge bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT ingeborgevanzeggeren bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT martindeijs bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT diederikvandebeek bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT matthijscbrouwer bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach AT liavanderhoek bacterialribosomalrnadetectionincerebrospinalfluidusingaviromicsapproach |