Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma
ObjectiveIntrahepatic cholangiocarcinoma (ICC) is a silent liver malignancy with an increasing incidence. Gut mycobiota plays a crucial role in benign liver diseases; however, its correlation with ICC remains elusive. This study aimed to elucidate fungal differences in patients with ICC compared to...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1090392/full |
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| author | Lilong Zhang Lilong Zhang Lilong Zhang Chen Chen Chen Chen Chen Chen Dongqi Chai Dongqi Chai Dongqi Chai Tianrui Kuang Tianrui Kuang Tianrui Kuang Wenhong Deng Wenhong Deng Wenhong Deng Weixing Wang Weixing Wang Weixing Wang |
| author_facet | Lilong Zhang Lilong Zhang Lilong Zhang Chen Chen Chen Chen Chen Chen Dongqi Chai Dongqi Chai Dongqi Chai Tianrui Kuang Tianrui Kuang Tianrui Kuang Wenhong Deng Wenhong Deng Wenhong Deng Weixing Wang Weixing Wang Weixing Wang |
| author_sort | Lilong Zhang |
| collection | DOAJ |
| description | ObjectiveIntrahepatic cholangiocarcinoma (ICC) is a silent liver malignancy with an increasing incidence. Gut mycobiota plays a crucial role in benign liver diseases; however, its correlation with ICC remains elusive. This study aimed to elucidate fungal differences in patients with ICC compared to healthy controls.MethodsThe 40 fecal samples from 23 ICC patients and 17 healthy controls were collected and analyzed using ITS2 rDNA sequencing. Obtaining the OTUs and combining effective grouping, we carried out the biodiversity and composition of the fungi, as well as FUNGuild functional annotation.ResultsOur results revealed the presence of intestinal fungal dysbiosis with significant enrichment of opportunistic pathogenic fungi such as Candida and C. albicans, and significant depletion of the beneficial fungus Saccharomyces cerevisiae in ICC patients compared with healthy controls. Alpha-diversity analysis demonstrated that patients with ICC showed decreased fungal diversity compared to healthy controls. Beta diversity analysis indicated that the two groups exhibited significant segregated clustering. Besides, C. albicans was found to be significantly more abundant in the ICC patients with TNM stage III-IV than those with stage I-II. The FUNGuild functional classification predicted that pathotrophs were the most abundant taxon in the ICC group, well above their abundance in healthy controls.ConclusionThis study indicates that dysbiosis of the fecal mycobiome might be involved in ICC development. Further research into gut fungi may contribute to new therapeutic options for ICC patients. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T00:35:38Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-28236fbdf20b46aea0cc253cf621e3122023-01-06T18:28:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10903921090392Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinomaLilong Zhang0Lilong Zhang1Lilong Zhang2Chen Chen3Chen Chen4Chen Chen5Dongqi Chai6Dongqi Chai7Dongqi Chai8Tianrui Kuang9Tianrui Kuang10Tianrui Kuang11Wenhong Deng12Wenhong Deng13Wenhong Deng14Weixing Wang15Weixing Wang16Weixing Wang17Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaObjectiveIntrahepatic cholangiocarcinoma (ICC) is a silent liver malignancy with an increasing incidence. Gut mycobiota plays a crucial role in benign liver diseases; however, its correlation with ICC remains elusive. This study aimed to elucidate fungal differences in patients with ICC compared to healthy controls.MethodsThe 40 fecal samples from 23 ICC patients and 17 healthy controls were collected and analyzed using ITS2 rDNA sequencing. Obtaining the OTUs and combining effective grouping, we carried out the biodiversity and composition of the fungi, as well as FUNGuild functional annotation.ResultsOur results revealed the presence of intestinal fungal dysbiosis with significant enrichment of opportunistic pathogenic fungi such as Candida and C. albicans, and significant depletion of the beneficial fungus Saccharomyces cerevisiae in ICC patients compared with healthy controls. Alpha-diversity analysis demonstrated that patients with ICC showed decreased fungal diversity compared to healthy controls. Beta diversity analysis indicated that the two groups exhibited significant segregated clustering. Besides, C. albicans was found to be significantly more abundant in the ICC patients with TNM stage III-IV than those with stage I-II. The FUNGuild functional classification predicted that pathotrophs were the most abundant taxon in the ICC group, well above their abundance in healthy controls.ConclusionThis study indicates that dysbiosis of the fecal mycobiome might be involved in ICC development. Further research into gut fungi may contribute to new therapeutic options for ICC patients.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1090392/fullgut mycobiomeintrahepatic cholangiocarcinomaITS2 rDNA sequencingdysbiosisCandida albicans |
| spellingShingle | Lilong Zhang Lilong Zhang Lilong Zhang Chen Chen Chen Chen Chen Chen Dongqi Chai Dongqi Chai Dongqi Chai Tianrui Kuang Tianrui Kuang Tianrui Kuang Wenhong Deng Wenhong Deng Wenhong Deng Weixing Wang Weixing Wang Weixing Wang Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma Frontiers in Microbiology gut mycobiome intrahepatic cholangiocarcinoma ITS2 rDNA sequencing dysbiosis Candida albicans |
| title | Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| title_full | Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| title_fullStr | Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| title_full_unstemmed | Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| title_short | Alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| title_sort | alterations of gut mycobiota profiles in intrahepatic cholangiocarcinoma |
| topic | gut mycobiome intrahepatic cholangiocarcinoma ITS2 rDNA sequencing dysbiosis Candida albicans |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1090392/full |
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