| Summary: | <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their antibacterial, antibiofilm, and anti-virulence actions against the opportunistic pathogen <i>Staphylococcus aureus</i>. The cytotoxic effect of agents tested against this bacterium was investigated by monitoring reactive oxygen-species (ROS) generation and antioxidant-enzyme (catalase) production. Regarding the antistaphylococcal effects, our results showed antibacterial efficacy of both Eos and their combination, where the minimum inhibitory concentrations ranged between 0.7 and 11.25 mg/mL. A combination of tested agents showed the highest anti-hemolytic and anti-protease effects. Additionally, association between EOs displayed more potency against the development of biofilm performed by <i>S. aureus,</i> with percentage of removal reaching 74%. The inhibitory impacts of EOs on <i>S. aureus</i> virulence factors were discovered to be concentration-dependent. Furthermore, our results provide insight on the abilities of <i>R. officinalis</i> and <i>M. communis</i> EOs, as well as their potential in combination, to generate ROS and affect oxidative stress enzyme catalase in <i>S. aureus</i>, leading to their antagonistic effect against this pathogen.
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