Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress
<i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/266 |
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| author | Khadijetou Hamoud Bowbe Karima Bel Hadj Salah Sarra Moumni Mada F. Ashkan Abderrahmen Merghni |
| author_facet | Khadijetou Hamoud Bowbe Karima Bel Hadj Salah Sarra Moumni Mada F. Ashkan Abderrahmen Merghni |
| author_sort | Khadijetou Hamoud Bowbe |
| collection | DOAJ |
| description | <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their antibacterial, antibiofilm, and anti-virulence actions against the opportunistic pathogen <i>Staphylococcus aureus</i>. The cytotoxic effect of agents tested against this bacterium was investigated by monitoring reactive oxygen-species (ROS) generation and antioxidant-enzyme (catalase) production. Regarding the antistaphylococcal effects, our results showed antibacterial efficacy of both Eos and their combination, where the minimum inhibitory concentrations ranged between 0.7 and 11.25 mg/mL. A combination of tested agents showed the highest anti-hemolytic and anti-protease effects. Additionally, association between EOs displayed more potency against the development of biofilm performed by <i>S. aureus,</i> with percentage of removal reaching 74%. The inhibitory impacts of EOs on <i>S. aureus</i> virulence factors were discovered to be concentration-dependent. Furthermore, our results provide insight on the abilities of <i>R. officinalis</i> and <i>M. communis</i> EOs, as well as their potential in combination, to generate ROS and affect oxidative stress enzyme catalase in <i>S. aureus</i>, leading to their antagonistic effect against this pathogen. |
| first_indexed | 2024-03-11T09:14:46Z |
| format | Article |
| id | doaj.art-2825e54035f847c88ffaff283ef56e27 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:14:46Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-2825e54035f847c88ffaff283ef56e272023-11-16T18:42:34ZengMDPI AGAntibiotics2079-63822023-01-0112226610.3390/antibiotics12020266Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative StressKhadijetou Hamoud Bowbe0Karima Bel Hadj Salah1Sarra Moumni2Mada F. Ashkan3Abderrahmen Merghni4Laboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy, University of Monastir, Monastir 5000, TunisiaLaboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy, University of Monastir, Monastir 5000, TunisiaLaboratory of Pharmaceutical, Chemical and Pharmacological Drug Development LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, TunisiaBiological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaLaboratory of Antimicrobial Resistance LR99ES09, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia<i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their antibacterial, antibiofilm, and anti-virulence actions against the opportunistic pathogen <i>Staphylococcus aureus</i>. The cytotoxic effect of agents tested against this bacterium was investigated by monitoring reactive oxygen-species (ROS) generation and antioxidant-enzyme (catalase) production. Regarding the antistaphylococcal effects, our results showed antibacterial efficacy of both Eos and their combination, where the minimum inhibitory concentrations ranged between 0.7 and 11.25 mg/mL. A combination of tested agents showed the highest anti-hemolytic and anti-protease effects. Additionally, association between EOs displayed more potency against the development of biofilm performed by <i>S. aureus,</i> with percentage of removal reaching 74%. The inhibitory impacts of EOs on <i>S. aureus</i> virulence factors were discovered to be concentration-dependent. Furthermore, our results provide insight on the abilities of <i>R. officinalis</i> and <i>M. communis</i> EOs, as well as their potential in combination, to generate ROS and affect oxidative stress enzyme catalase in <i>S. aureus</i>, leading to their antagonistic effect against this pathogen.https://www.mdpi.com/2079-6382/12/2/266essential oil<i>R. officinalis</i><i>M. communis</i><i>Staphylococcus aureus</i>ant-virulenceoxidative stress |
| spellingShingle | Khadijetou Hamoud Bowbe Karima Bel Hadj Salah Sarra Moumni Mada F. Ashkan Abderrahmen Merghni Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress Antibiotics essential oil <i>R. officinalis</i> <i>M. communis</i> <i>Staphylococcus aureus</i> ant-virulence oxidative stress |
| title | Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress |
| title_full | Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress |
| title_fullStr | Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress |
| title_full_unstemmed | Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress |
| title_short | Anti-Staphylococcal Activities of <i>Rosmarinus officinalis</i> and <i>Myrtus communis</i> Essential Oils through ROS-Mediated Oxidative Stress |
| title_sort | anti staphylococcal activities of i rosmarinus officinalis i and i myrtus communis i essential oils through ros mediated oxidative stress |
| topic | essential oil <i>R. officinalis</i> <i>M. communis</i> <i>Staphylococcus aureus</i> ant-virulence oxidative stress |
| url | https://www.mdpi.com/2079-6382/12/2/266 |
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