Vancomycin and high-level aminoglycoside resistance in Enterococcus species
The aim of the study was to investigate vancomycin and high-level aminoglycoside resistance (HLAR) in <em>Enterococcus</em> species by phenotypic and genotypic methods. A hundred <em>Enterococcus</em> strains were included in the study. Antimicrobial susceptibilities of strai...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/mr/article/view/6441 |
| _version_ | 1819275699862634496 |
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| author | Seyda Ozarslan Kurtgoz Burcin Ozer Melek Inci Nizami Duran Erkan Yula |
| author_facet | Seyda Ozarslan Kurtgoz Burcin Ozer Melek Inci Nizami Duran Erkan Yula |
| author_sort | Seyda Ozarslan Kurtgoz |
| collection | DOAJ |
| description | The aim of the study was to investigate vancomycin and high-level aminoglycoside resistance (HLAR) in <em>Enterococcus</em> species by phenotypic and genotypic methods. A hundred <em>Enterococcus</em> strains were included in the study. Antimicrobial susceptibilities of strains were investigated by automated system, betalactamase production was investigated by nitrocefin disks, vancomycin resistance and HLAR were investigated by gradient diffusion method (GDM) and disk diffusion method, respectively. For detection of vancomycin and high-level gentamicin resistance (HLGR) genes, polymerase chain reaction was used. Teicoplanin linezolid, vancomycin, ampicillin, penicillin are the most susceptible antibiotics and strains were detected not to produce beta lactamase. Vancomycin resistance was detected in ten isolates by automated system and in only five isolates by GDM. Five isolates carrying <em>VanA</em> gene were determined. The ratio of HLGR and high-level streptomycin resistance was found 40 and 63% respectively. <em>aac (6’)-1eaph (2’’)-1a</em> gene was detected in 58% of strains. <em>E. faecium</em> strains were found more resistant to the antibiotics than the other species. Beta lactamase was detected in none of strains. The automated system detected vancomycin resistance in more strains than GDM. Therefore it is concluded that strains, which were detected to be resistant to vancomycin, should be confirmed by GDM. The ratio of <em>VanA</em> gene in strains is consistent with other studies. The HLAR ratio was found in about half of strains. The ratio of<em> aac(6’)-1e-aph(2’’)-1a</em> gene, which is the most reported gene in our country and other countries and one of the HLGR genes investigated in our study, was detected 58%. |
| first_indexed | 2024-12-23T23:28:29Z |
| format | Article |
| id | doaj.art-282c4dac6bb5456f96c8d7a437f2a179 |
| institution | Directory Open Access Journal |
| issn | 2036-7473 2036-7481 |
| language | English |
| last_indexed | 2024-12-23T23:28:29Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-282c4dac6bb5456f96c8d7a437f2a1792022-12-21T17:26:09ZengMDPI AGMicrobiology Research2036-74732036-74812016-07-017110.4081/mr.2016.64413474Vancomycin and high-level aminoglycoside resistance in Enterococcus speciesSeyda Ozarslan Kurtgoz0Burcin Ozer1Melek Inci2Nizami Duran3Erkan Yula4City Hospital, Turhal, TokatDepartment of Medical Microbiology, Mustafa Kemal University, School of Medicine, HatayDepartment of Medical Microbiology, Mustafa Kemal University, School of Medicine, HatayDepartment of Medical Microbiology, Mustafa Kemal University, School of Medicine, HatayDepartment of Medical Microbiology, Katip Celebi University, School of Medicine, IzmirThe aim of the study was to investigate vancomycin and high-level aminoglycoside resistance (HLAR) in <em>Enterococcus</em> species by phenotypic and genotypic methods. A hundred <em>Enterococcus</em> strains were included in the study. Antimicrobial susceptibilities of strains were investigated by automated system, betalactamase production was investigated by nitrocefin disks, vancomycin resistance and HLAR were investigated by gradient diffusion method (GDM) and disk diffusion method, respectively. For detection of vancomycin and high-level gentamicin resistance (HLGR) genes, polymerase chain reaction was used. Teicoplanin linezolid, vancomycin, ampicillin, penicillin are the most susceptible antibiotics and strains were detected not to produce beta lactamase. Vancomycin resistance was detected in ten isolates by automated system and in only five isolates by GDM. Five isolates carrying <em>VanA</em> gene were determined. The ratio of HLGR and high-level streptomycin resistance was found 40 and 63% respectively. <em>aac (6’)-1eaph (2’’)-1a</em> gene was detected in 58% of strains. <em>E. faecium</em> strains were found more resistant to the antibiotics than the other species. Beta lactamase was detected in none of strains. The automated system detected vancomycin resistance in more strains than GDM. Therefore it is concluded that strains, which were detected to be resistant to vancomycin, should be confirmed by GDM. The ratio of <em>VanA</em> gene in strains is consistent with other studies. The HLAR ratio was found in about half of strains. The ratio of<em> aac(6’)-1e-aph(2’’)-1a</em> gene, which is the most reported gene in our country and other countries and one of the HLGR genes investigated in our study, was detected 58%.http://www.pagepress.org/journals/index.php/mr/article/view/6441Enterococcusbeta lactamasevancomycinhigh-level aminoglycosideresistance |
| spellingShingle | Seyda Ozarslan Kurtgoz Burcin Ozer Melek Inci Nizami Duran Erkan Yula Vancomycin and high-level aminoglycoside resistance in Enterococcus species Microbiology Research Enterococcus beta lactamase vancomycin high-level aminoglycoside resistance |
| title | Vancomycin and high-level aminoglycoside resistance in Enterococcus species |
| title_full | Vancomycin and high-level aminoglycoside resistance in Enterococcus species |
| title_fullStr | Vancomycin and high-level aminoglycoside resistance in Enterococcus species |
| title_full_unstemmed | Vancomycin and high-level aminoglycoside resistance in Enterococcus species |
| title_short | Vancomycin and high-level aminoglycoside resistance in Enterococcus species |
| title_sort | vancomycin and high level aminoglycoside resistance in enterococcus species |
| topic | Enterococcus beta lactamase vancomycin high-level aminoglycoside resistance |
| url | http://www.pagepress.org/journals/index.php/mr/article/view/6441 |
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