Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS
(1) Background: Citrus honey constitutes a unique monofloral honey characterized by a distinctive aroma and unique taste. The non-targeted chemical analysis can provide pivotal information on chemical markers that differentiate honey based on its geographical and botanical origin. (2) Methods: Withi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/9/3967 |
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| author | Konstantinos M. Kasiotis Eirini Baira Styliani Iosifidou Electra Manea-Karga Despina Tsipi Sofia Gounari Ioannis Theologidis Theodora Barmpouni Pier Paolo Danieli Filippo Lazzari Daniele Dipasquale Sonia Petrarca Souad Shairra Naglaa A. Ghazala Aida A. Abd El-Wahed Seham M. A. El-Gamal Kyriaki Machera |
| author_facet | Konstantinos M. Kasiotis Eirini Baira Styliani Iosifidou Electra Manea-Karga Despina Tsipi Sofia Gounari Ioannis Theologidis Theodora Barmpouni Pier Paolo Danieli Filippo Lazzari Daniele Dipasquale Sonia Petrarca Souad Shairra Naglaa A. Ghazala Aida A. Abd El-Wahed Seham M. A. El-Gamal Kyriaki Machera |
| author_sort | Konstantinos M. Kasiotis |
| collection | DOAJ |
| description | (1) Background: Citrus honey constitutes a unique monofloral honey characterized by a distinctive aroma and unique taste. The non-targeted chemical analysis can provide pivotal information on chemical markers that differentiate honey based on its geographical and botanical origin. (2) Methods: Within the PRIMA project “PLANT-B”, a metabolomics workflow was established to unveil potential chemical markers of orange blossom honey produced in case study areas of Egypt, Italy, and Greece. In some of these areas, aromatic medicinal plants were cultivated to enhance biodiversity and attract pollinators. The non-targeted chemical analysis and metabolomics were conducted using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). (3) Results: Forty compounds were disclosed as potential chemical markers, enabling the differentiation of the three orange blossom honeys according to geographical origin. Italian honey showed a preponderance of flavonoids, while in Greek honey, terpenoids and iridoids were more abundant than flavonoids, except for hesperidin. In Egyptian honey, suberic acid and a fatty acid ester derivative emerged as chemical markers. New, for honey, furan derivatives were identified using GC-MS in Greek samples. (4) Conclusions: The application of UHPLC-HRMS metabolomics combined with an elaborate melissopalynological analysis managed to unveil several potential markers of Mediterranean citrus honey potentially associated with citrus crop varieties and the local indigenous flora. |
| first_indexed | 2024-03-11T04:10:48Z |
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| id | doaj.art-2833cf193c1e4f14abe85a5dfca5c9f5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:10:48Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2833cf193c1e4f14abe85a5dfca5c9f52023-11-17T23:25:54ZengMDPI AGMolecules1420-30492023-05-01289396710.3390/molecules28093967Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MSKonstantinos M. Kasiotis0Eirini Baira1Styliani Iosifidou2Electra Manea-Karga3Despina Tsipi4Sofia Gounari5Ioannis Theologidis6Theodora Barmpouni7Pier Paolo Danieli8Filippo Lazzari9Daniele Dipasquale10Sonia Petrarca11Souad Shairra12Naglaa A. Ghazala13Aida A. Abd El-Wahed14Seham M. A. El-Gamal15Kyriaki Machera16Laboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, GreeceLaboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, GreeceGeneral Chemical State Laboratory, Independent Public Revenue Authority (A.A.D.E.), 16 An. Tsocha Street, 115 21 Athens, GreeceLaboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, GreeceGeneral Chemical State Laboratory, Independent Public Revenue Authority (A.A.D.E.), 16 An. Tsocha Street, 115 21 Athens, GreeceLaboratory of Apiculture, Institute of Mediterranean & Forest Ecosystems, ELGO DHMHTRA, 115 28 Athens, GreeceLaboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, GreeceLaboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, GreeceDepartment of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via. S. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via. S. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via. S. Camillo de Lellis snc, 01100 Viterbo, ItalyConsorzio Nazionale Produttori Apistici (CONAPROA), Via N. Guerrizio, 2, 86100 Campobasso, ItalyBiological Control Department, Plant Protection Research Institute, Agricultural Research Center, Giza 12619, EgyptDepartment of Bee Research, Plant Protection Research Institute, Agricultural Research Center, Giza 12627, EgyptDepartment of Bee Research, Plant Protection Research Institute, Agricultural Research Center, Giza 12627, EgyptMedicinal and Aromatic Plants Research Department, Horticulture Research Institute, Agricultural Research Center, Giza 12619, EgyptLaboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61 Kifissia, Greece(1) Background: Citrus honey constitutes a unique monofloral honey characterized by a distinctive aroma and unique taste. The non-targeted chemical analysis can provide pivotal information on chemical markers that differentiate honey based on its geographical and botanical origin. (2) Methods: Within the PRIMA project “PLANT-B”, a metabolomics workflow was established to unveil potential chemical markers of orange blossom honey produced in case study areas of Egypt, Italy, and Greece. In some of these areas, aromatic medicinal plants were cultivated to enhance biodiversity and attract pollinators. The non-targeted chemical analysis and metabolomics were conducted using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). (3) Results: Forty compounds were disclosed as potential chemical markers, enabling the differentiation of the three orange blossom honeys according to geographical origin. Italian honey showed a preponderance of flavonoids, while in Greek honey, terpenoids and iridoids were more abundant than flavonoids, except for hesperidin. In Egyptian honey, suberic acid and a fatty acid ester derivative emerged as chemical markers. New, for honey, furan derivatives were identified using GC-MS in Greek samples. (4) Conclusions: The application of UHPLC-HRMS metabolomics combined with an elaborate melissopalynological analysis managed to unveil several potential markers of Mediterranean citrus honey potentially associated with citrus crop varieties and the local indigenous flora.https://www.mdpi.com/1420-3049/28/9/3967citrushoneyaromatic medicinal plantspollenmetabolomicsmass spectrometry |
| spellingShingle | Konstantinos M. Kasiotis Eirini Baira Styliani Iosifidou Electra Manea-Karga Despina Tsipi Sofia Gounari Ioannis Theologidis Theodora Barmpouni Pier Paolo Danieli Filippo Lazzari Daniele Dipasquale Sonia Petrarca Souad Shairra Naglaa A. Ghazala Aida A. Abd El-Wahed Seham M. A. El-Gamal Kyriaki Machera Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS Molecules citrus honey aromatic medicinal plants pollen metabolomics mass spectrometry |
| title | Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS |
| title_full | Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS |
| title_fullStr | Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS |
| title_full_unstemmed | Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS |
| title_short | Fingerprinting Chemical Markers in the Mediterranean Orange Blossom Honey: UHPLC-HRMS Metabolomics Study Integrating Melissopalynological Analysis, GC-MS and HPLC-PDA-ESI/MS |
| title_sort | fingerprinting chemical markers in the mediterranean orange blossom honey uhplc hrms metabolomics study integrating melissopalynological analysis gc ms and hplc pda esi ms |
| topic | citrus honey aromatic medicinal plants pollen metabolomics mass spectrometry |
| url | https://www.mdpi.com/1420-3049/28/9/3967 |
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