Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs
Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2014-10-01
|
| Series: | Burns & Trauma |
| Subjects: | |
| Online Access: | http://www.burnstrauma.com/article.asp?issn=2321-3868;year=2014;volume=2;issue=4;spage=181;epage=186;aulast=Wang |
| _version_ | 1818172198255329280 |
|---|---|
| author | Huili Wang Xin Yan Liangyun Shen Shiyan Li Yue Lin Shuqin Wang Xiang Lin Hou Chunying Shi Yun Yang Jianwu Dai Qian Tan |
| author_facet | Huili Wang Xin Yan Liangyun Shen Shiyan Li Yue Lin Shuqin Wang Xiang Lin Hou Chunying Shi Yun Yang Jianwu Dai Qian Tan |
| author_sort | Huili Wang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. <i>In vivo</i> investigations indicated that due to the immunoreaction, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups (<i>P</i> < 0.05, <i>P</i> < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds. |
| first_indexed | 2024-12-11T19:08:48Z |
| format | Article |
| id | doaj.art-2833e395176646bdaf6d1777b8bb7ced |
| institution | Directory Open Access Journal |
| issn | 2321-3868 2321-3876 |
| language | English |
| last_indexed | 2024-12-11T19:08:48Z |
| publishDate | 2014-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Burns & Trauma |
| spelling | doaj.art-2833e395176646bdaf6d1777b8bb7ced2022-12-22T00:53:49ZengOxford University PressBurns & Trauma2321-38682321-38762014-10-012418118610.4103/2321-3868.143623Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCsHuili WangXin YanLiangyun ShenShiyan Li Yue LinShuqin WangXiang Lin HouChunying ShiYun YangJianwu DaiQian TanMesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. <i>In vivo</i> investigations indicated that due to the immunoreaction, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups (<i>P</i> < 0.05, <i>P</i> < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds.http://www.burnstrauma.com/article.asp?issn=2321-3868;year=2014;volume=2;issue=4;spage=181;epage=186;aulast=WangMesenchymal stem cellsskin wound healingCollagen-binding domainmesenchymal stem cells affinity peptide |
| spellingShingle | Huili Wang Xin Yan Liangyun Shen Shiyan Li Yue Lin Shuqin Wang Xiang Lin Hou Chunying Shi Yun Yang Jianwu Dai Qian Tan Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs Burns & Trauma Mesenchymal stem cells skin wound healing Collagen-binding domain mesenchymal stem cells affinity peptide |
| title | Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs |
| title_full | Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs |
| title_fullStr | Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs |
| title_full_unstemmed | Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs |
| title_short | Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs |
| title_sort | acceleration of wound healing in acute full thickness skin wounds using a collagen binding peptide with an affinity for mscs |
| topic | Mesenchymal stem cells skin wound healing Collagen-binding domain mesenchymal stem cells affinity peptide |
| url | http://www.burnstrauma.com/article.asp?issn=2321-3868;year=2014;volume=2;issue=4;spage=181;epage=186;aulast=Wang |
| work_keys_str_mv | AT huiliwang accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT xinyan accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT liangyunshen accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT shiyanli accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT yuelin accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT shuqinwang accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT xianglinhou accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT chunyingshi accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT yunyang accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT jianwudai accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs AT qiantan accelerationofwoundhealinginacutefullthicknessskinwoundsusingacollagenbindingpeptidewithanaffinityformscs |