Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
Abstract Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screen...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13722-z |
| _version_ | 1818249812485603328 |
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| author | Sebnem Hazal Gulsen Evren Tileklioglu Edna Bode Harun Cimen Hatice Ertabaklar Derya Ulug Sema Ertug Sebastian L. Wenski Mustapha Touray Canan Hazir Duygu Kaya Bilecenoglu Ibrahim Yildiz Helge B. Bode Selcuk Hazir |
| author_facet | Sebnem Hazal Gulsen Evren Tileklioglu Edna Bode Harun Cimen Hatice Ertabaklar Derya Ulug Sema Ertug Sebastian L. Wenski Mustapha Touray Canan Hazir Duygu Kaya Bilecenoglu Ibrahim Yildiz Helge B. Bode Selcuk Hazir |
| author_sort | Sebnem Hazal Gulsen |
| collection | DOAJ |
| description | Abstract Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future. |
| first_indexed | 2024-12-12T15:42:26Z |
| format | Article |
| id | doaj.art-2834b885765c4fc79432f5af53f556d3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T15:42:26Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2834b885765c4fc79432f5af53f556d32022-12-22T00:19:51ZengNature PortfolioScientific Reports2045-23222022-06-0112111310.1038/s41598-022-13722-zAntiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approachSebnem Hazal Gulsen0Evren Tileklioglu1Edna Bode2Harun Cimen3Hatice Ertabaklar4Derya Ulug5Sema Ertug6Sebastian L. Wenski7Mustapha Touray8Canan Hazir9Duygu Kaya Bilecenoglu10Ibrahim Yildiz11Helge B. Bode12Selcuk Hazir13Department of Biology, Faculty of Arts and Science, Aydin Adnan Menderes UniversityDepartment of Parasitology, Faculty of Medicine, Aydin Adnan Menderes UniversityMax-Planck-Institute for Terrestrial Microbiology Department, Natural Products in Organismic InteractionsDepartment of Biology, Faculty of Arts and Science, Aydin Adnan Menderes UniversityDepartment of Parasitology, Faculty of Medicine, Aydin Adnan Menderes UniversityDepartment of Biology, Faculty of Arts and Science, Aydin Adnan Menderes UniversityDepartment of Parasitology, Faculty of Medicine, Aydin Adnan Menderes UniversityMax-Planck-Institute for Terrestrial Microbiology Department, Natural Products in Organismic InteractionsDepartment of Biology, Faculty of Arts and Science, Aydin Adnan Menderes UniversityAydin Health Services Vocational School, Aydin Adnan Menderes UniversityFaculty of Health Science, Aydin Adnan Menderes UniversityDepartment of Parasitology, Faculty of Medicine, Aydin Adnan Menderes UniversityMax-Planck-Institute for Terrestrial Microbiology Department, Natural Products in Organismic InteractionsDepartment of Biology, Faculty of Arts and Science, Aydin Adnan Menderes UniversityAbstract Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.https://doi.org/10.1038/s41598-022-13722-z |
| spellingShingle | Sebnem Hazal Gulsen Evren Tileklioglu Edna Bode Harun Cimen Hatice Ertabaklar Derya Ulug Sema Ertug Sebastian L. Wenski Mustapha Touray Canan Hazir Duygu Kaya Bilecenoglu Ibrahim Yildiz Helge B. Bode Selcuk Hazir Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach Scientific Reports |
| title | Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach |
| title_full | Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach |
| title_fullStr | Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach |
| title_full_unstemmed | Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach |
| title_short | Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach |
| title_sort | antiprotozoal activity of different xenorhabdus and photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easypacid approach |
| url | https://doi.org/10.1038/s41598-022-13722-z |
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