<i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice
As part of the search for new bioactive plants from the Colombian Caribbean, the dichloromethane fraction of the calyces of <i>Physalis angulata</i> L. (PADF) was selected for its anti-inflammatory activity. In this work, we investigated the immunomodulatory effect of PADF in activated m...
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MDPI AG
2020-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/2/24 |
| _version_ | 1818310468561797120 |
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| author | David Rivera Yanet Ocampo Luis A. Franco |
| author_facet | David Rivera Yanet Ocampo Luis A. Franco |
| author_sort | David Rivera |
| collection | DOAJ |
| description | As part of the search for new bioactive plants from the Colombian Caribbean, the dichloromethane fraction of the calyces of <i>Physalis angulata</i> L. (PADF) was selected for its anti-inflammatory activity. In this work, we investigated the immunomodulatory effect of PADF in activated macrophages and during dextran sulfate sodium (DSS)-induced colitis. PADF displayed a low content of withanolides or phenolic compounds, and a higher content of sucrose esters, representative anti-inflammatory metabolites of the <i>Physalis</i> genus. The PADF fraction at 12.5 μg/mL prevented the induction of interleukin (IL)-1β, tumor necrosis factor (TNF-α), IL-6, IL-12, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) by lipopolysaccharide (LPS), while increased the levels of arginase (ARG1), IL-10, and mannose receptor C (MRC1). The polarization towards an anti-inflammatory profile was also observed in resting macrophages, without promoting the typical gene profile induced by IL-4, suggesting that PADF promotes a shift to a regulatory status rather than to an alternative one. In vivo, the administration of PADF to mice with chronic DSS-colitis reduced disease signs (i.e., body weight loss and colon shortening), and improved the histology score by diminishing the levels of pro-inflammatory cytokines and increasing the production of IL-10. Overall, results suggest that the regulatory effect on PADF towards macrophages might contribute to the therapeutic activity observed in the murine model of inflammatory bowel disease. |
| first_indexed | 2024-12-13T07:46:33Z |
| format | Article |
| id | doaj.art-283656f5bbbc4db98ddda62f323e7932 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-12-13T07:46:33Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-283656f5bbbc4db98ddda62f323e79322022-12-21T23:54:48ZengMDPI AGBiomedicines2227-90592020-02-01822410.3390/biomedicines8020024biomedicines8020024<i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in MiceDavid Rivera0Yanet Ocampo1Luis A. Franco2Biological Evaluation of Promising Substances Group, Department of Pharmaceutical Sciences, University of Cartagena, Carrera 50 No. 29-11, Cartagena 130014, ColombiaBiological Evaluation of Promising Substances Group, Department of Pharmaceutical Sciences, University of Cartagena, Carrera 50 No. 29-11, Cartagena 130014, ColombiaBiological Evaluation of Promising Substances Group, Department of Pharmaceutical Sciences, University of Cartagena, Carrera 50 No. 29-11, Cartagena 130014, ColombiaAs part of the search for new bioactive plants from the Colombian Caribbean, the dichloromethane fraction of the calyces of <i>Physalis angulata</i> L. (PADF) was selected for its anti-inflammatory activity. In this work, we investigated the immunomodulatory effect of PADF in activated macrophages and during dextran sulfate sodium (DSS)-induced colitis. PADF displayed a low content of withanolides or phenolic compounds, and a higher content of sucrose esters, representative anti-inflammatory metabolites of the <i>Physalis</i> genus. The PADF fraction at 12.5 μg/mL prevented the induction of interleukin (IL)-1β, tumor necrosis factor (TNF-α), IL-6, IL-12, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) by lipopolysaccharide (LPS), while increased the levels of arginase (ARG1), IL-10, and mannose receptor C (MRC1). The polarization towards an anti-inflammatory profile was also observed in resting macrophages, without promoting the typical gene profile induced by IL-4, suggesting that PADF promotes a shift to a regulatory status rather than to an alternative one. In vivo, the administration of PADF to mice with chronic DSS-colitis reduced disease signs (i.e., body weight loss and colon shortening), and improved the histology score by diminishing the levels of pro-inflammatory cytokines and increasing the production of IL-10. Overall, results suggest that the regulatory effect on PADF towards macrophages might contribute to the therapeutic activity observed in the murine model of inflammatory bowel disease.https://www.mdpi.com/2227-9059/8/2/24<i>physalis angulata</i>macrophagesinflammatory bowel disease |
| spellingShingle | David Rivera Yanet Ocampo Luis A. Franco <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice Biomedicines <i>physalis angulata</i> macrophages inflammatory bowel disease |
| title | <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice |
| title_full | <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice |
| title_fullStr | <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice |
| title_full_unstemmed | <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice |
| title_short | <i>Physalis angulata</i> Calyces Modulate Macrophage Polarization and Alleviate Chemically Induced Intestinal Inflammation in Mice |
| title_sort | i physalis angulata i calyces modulate macrophage polarization and alleviate chemically induced intestinal inflammation in mice |
| topic | <i>physalis angulata</i> macrophages inflammatory bowel disease |
| url | https://www.mdpi.com/2227-9059/8/2/24 |
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