Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways
IntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associa...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2024.1373499/full |
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| author | Natália Yumi Noronha Natália Yumi Noronha Isabella Harumi Yonehara Noma Rafael Fernandes Ferreira Guilherme da Silva Rodrigues Luzania dos Santos Martins Lígia Moriguchi Watanabe Marcela Augusta de Souza Pinhel Marcela Augusta de Souza Pinhel Isabelle Mello Schineider Luísa Maria Diani Daniela Carlos Carla Barbosa Nonino |
| author_facet | Natália Yumi Noronha Natália Yumi Noronha Isabella Harumi Yonehara Noma Rafael Fernandes Ferreira Guilherme da Silva Rodrigues Luzania dos Santos Martins Lígia Moriguchi Watanabe Marcela Augusta de Souza Pinhel Marcela Augusta de Souza Pinhel Isabelle Mello Schineider Luísa Maria Diani Daniela Carlos Carla Barbosa Nonino |
| author_sort | Natália Yumi Noronha |
| collection | DOAJ |
| description | IntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.MethodsSix healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.Results and discussionThe group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables (r = 0.84, p = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response. |
| first_indexed | 2024-04-24T13:22:19Z |
| format | Article |
| id | doaj.art-2836c483fb7a414f8d382af11424fe54 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-24T13:22:19Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-2836c483fb7a414f8d382af11424fe542024-04-04T10:25:36ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-04-011110.3389/fnut.2024.13734991373499Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathwaysNatália Yumi Noronha0Natália Yumi Noronha1Isabella Harumi Yonehara Noma2Rafael Fernandes Ferreira3Guilherme da Silva Rodrigues4Luzania dos Santos Martins5Lígia Moriguchi Watanabe6Marcela Augusta de Souza Pinhel7Marcela Augusta de Souza Pinhel8Isabelle Mello Schineider9Luísa Maria Diani10Daniela Carlos11Carla Barbosa Nonino12Department of Gynecology and Obstetrics, University Medical Center Groningen, Groningen, NetherlandsFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Molecular Biology, Sao Jose do Rio Preto Medical School, São José do Rio Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirao Preto, Department of Health Sciences, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilDepartment of Molecular Biology, Sao Jose do Rio Preto Medical School, São José do Rio Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirão Preto, Department of Biochemistry and Immunology, University of São Paulo, Ribeirão Preto, BrazilFaculty of Medicine of Ribeirao Preto, Department of Health Sciences, University of São Paulo, Ribeirão Preto, BrazilIntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.MethodsSix healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.Results and discussionThe group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables (r = 0.84, p = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response.https://www.frontiersin.org/articles/10.3389/fnut.2024.1373499/fullDNA methylationActinobacteriavitamin Cimmune response and nutrientsepigenetic |
| spellingShingle | Natália Yumi Noronha Natália Yumi Noronha Isabella Harumi Yonehara Noma Rafael Fernandes Ferreira Guilherme da Silva Rodrigues Luzania dos Santos Martins Lígia Moriguchi Watanabe Marcela Augusta de Souza Pinhel Marcela Augusta de Souza Pinhel Isabelle Mello Schineider Luísa Maria Diani Daniela Carlos Carla Barbosa Nonino Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways Frontiers in Nutrition DNA methylation Actinobacteria vitamin C immune response and nutrients epigenetic |
| title | Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways |
| title_full | Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways |
| title_fullStr | Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways |
| title_full_unstemmed | Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways |
| title_short | Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways |
| title_sort | association between the relative abundance of phyla actinobacteria vitamin c consumption and dna methylation of genes linked to immune response pathways |
| topic | DNA methylation Actinobacteria vitamin C immune response and nutrients epigenetic |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2024.1373499/full |
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