Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury
Abstract Background The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this...
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| Format: | Article |
| Language: | English |
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Wiley
2018-06-01
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| Series: | Journal of Experimental Orthopaedics |
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| Online Access: | http://link.springer.com/article/10.1186/s40634-018-0130-z |
| _version_ | 1797280359853850624 |
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| author | Clarissa Rocha dos Santos Richard da Rocha Filgueiras Patrícia Furtado Malard Andre Rodrigues da Cunha Barreto-Vianna Kaique Nogueira Carolina da Silva Leite Eduardo Maurício Mendes de Lima |
| author_facet | Clarissa Rocha dos Santos Richard da Rocha Filgueiras Patrícia Furtado Malard Andre Rodrigues da Cunha Barreto-Vianna Kaique Nogueira Carolina da Silva Leite Eduardo Maurício Mendes de Lima |
| author_sort | Clarissa Rocha dos Santos |
| collection | DOAJ |
| description | Abstract Background The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. Methods The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups. Animals from the treated group (GT) received cell transport medium containing about 1.5 millions MSC, and the animals from the control group (GC) received only the cell transport medium. The study was performed in a double-blind manner using radiographs acquired on days 15, 30, 60 and 120 after the procedure. Evaluations of the density of the trabecular bone were performed using image analysis software. The results were subjected to descriptive statistical analysis, followed by the normality test, Chi-square test, Mann-Whitney test and Tukey’s multiple comparison test for p ≤ 0.05. Results After 30 days of the procedure, the animals of the GT presented an ossification mean 36.45% greater (p ≤ 0.033) than the GC, and there were no statistical differences for the other periods. Conclusions Despite the total bone ossification within the expected period, there was no minimization of the estimated recovery time with the application of MSC, and inflammatory factors should be considered for reassessment of the therapeutic intervention time. |
| first_indexed | 2024-03-07T16:39:08Z |
| format | Article |
| id | doaj.art-28439c41380248a9ab7174574d010fb3 |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-07T16:39:08Z |
| publishDate | 2018-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-28439c41380248a9ab7174574d010fb32024-03-03T08:37:47ZengWileyJournal of Experimental Orthopaedics2197-11532018-06-01511810.1186/s40634-018-0130-zMesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injuryClarissa Rocha dos Santos0Richard da Rocha Filgueiras1Patrícia Furtado Malard2Andre Rodrigues da Cunha Barreto-Vianna3Kaique Nogueira4Carolina da Silva Leite5Eduardo Maurício Mendes de Lima6Faculty of Agronomy and Veterinary Medicine, University of BrasiliaOrthos Ortopedia e Neurocirurgia VeterináriaBIO-Biotecnologia em Reprodução AnimalFaculty of Veterinary Medicine, Federal University of LavrasFaculty of Agronomy and Veterinary Medicine, University of BrasiliaFaculty of Agronomy and Veterinary Medicine, University of BrasiliaFaculty of Agronomy and Veterinary Medicine, University of BrasiliaAbstract Background The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. Methods The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups. Animals from the treated group (GT) received cell transport medium containing about 1.5 millions MSC, and the animals from the control group (GC) received only the cell transport medium. The study was performed in a double-blind manner using radiographs acquired on days 15, 30, 60 and 120 after the procedure. Evaluations of the density of the trabecular bone were performed using image analysis software. The results were subjected to descriptive statistical analysis, followed by the normality test, Chi-square test, Mann-Whitney test and Tukey’s multiple comparison test for p ≤ 0.05. Results After 30 days of the procedure, the animals of the GT presented an ossification mean 36.45% greater (p ≤ 0.033) than the GC, and there were no statistical differences for the other periods. Conclusions Despite the total bone ossification within the expected period, there was no minimization of the estimated recovery time with the application of MSC, and inflammatory factors should be considered for reassessment of the therapeutic intervention time.http://link.springer.com/article/10.1186/s40634-018-0130-zOrthopedicsBiotechnologyJointsKnee |
| spellingShingle | Clarissa Rocha dos Santos Richard da Rocha Filgueiras Patrícia Furtado Malard Andre Rodrigues da Cunha Barreto-Vianna Kaique Nogueira Carolina da Silva Leite Eduardo Maurício Mendes de Lima Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury Journal of Experimental Orthopaedics Orthopedics Biotechnology Joints Knee |
| title | Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| title_full | Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| title_fullStr | Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| title_full_unstemmed | Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| title_short | Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| title_sort | mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury |
| topic | Orthopedics Biotechnology Joints Knee |
| url | http://link.springer.com/article/10.1186/s40634-018-0130-z |
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