The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis
Inflammatory cytokines have crucial roles in the pathogenesis of tuberculosis (TB), and interleukin (IL)-27 and IL-35 have a pro-inflammatory and anti-inflammatory effect on many diseases, including infectious diseases. Therefore, we evaluated the relationship between IL-27 and IL-35 gene polymorphi...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1267624/full |
| _version_ | 1827281322160160768 |
|---|---|
| author | Lei Gao Lei Gao Yan-Jun Xiong Ya-Xue Liang Peng-Fei Huang Shuang Liu Yu Xiao Qian Huang Hua Wang Hui-Mei Wu |
| author_facet | Lei Gao Lei Gao Yan-Jun Xiong Ya-Xue Liang Peng-Fei Huang Shuang Liu Yu Xiao Qian Huang Hua Wang Hui-Mei Wu |
| author_sort | Lei Gao |
| collection | DOAJ |
| description | Inflammatory cytokines have crucial roles in the pathogenesis of tuberculosis (TB), and interleukin (IL)-27 and IL-35 have a pro-inflammatory and anti-inflammatory effect on many diseases, including infectious diseases. Therefore, we evaluated the relationship between IL-27 and IL-35 gene polymorphism, expression levels, and pulmonary TB (PTB) susceptibility. Nine single-nucleotide polymorphisms (SNPs) in the IL-27 gene (rs181206, rs153109, and rs17855750) and the IL-35 gene (rs4740, rs428253, rs9807813, rs2243123, rs2243135, and rs568408) were genotyped by the SNPscan technique in 497 patients with PTB and 501 controls. There was no significant difference regarding the genotype and allele frequencies of the above SNPs in the IL-27 and IL-35 genes between patients with PTB and controls. Haplotype analysis showed that the frequency of the GAC haplotype in the IL-35 gene was significantly decreased in patients with PTB when compared to controls (p = 0.036). Stratified analysis suggested that the frequency of the IL-27 rs17855750 GG genotype was significantly increased in patients with PTB with fever. Moreover, the lower frequency of the IL-35 rs568408 GA genotype was associated with drug-induced liver injury in patients with PTB. The IL-35 rs428253 GC genotype, as well as the rs4740 AA genotype and A allele, showed significant relationships with hypoproteinemia in patients with PTB. When compared with controls, the IL-27 level was significantly increased in patients with PTB. Taken together, IL-35 gene variation might contribute to a protective role on the susceptibility to PTB, and IL-27 and IL-35 gene polymorphisms were associated with several clinical manifestations of patients with PTB. |
| first_indexed | 2024-04-24T08:58:18Z |
| format | Article |
| id | doaj.art-28454619740542a79b11c42104a4a2e6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-24T08:58:18Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-28454619740542a79b11c42104a4a2e62024-04-16T04:51:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-04-011510.3389/fimmu.2024.12676241267624The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosisLei Gao0Lei Gao1Yan-Jun Xiong2Ya-Xue Liang3Peng-Fei Huang4Shuang Liu5Yu Xiao6Qian Huang7Hua Wang8Hui-Mei Wu9Anhui Geriatric Institute, Department of Geriatric Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaDepartment of Public Health, Anhui Medical University, Hefei, ChinaDepartment of Tuberculosis, Anhui Chest Hospital, Hefei, Anhui, ChinaAnhui Geriatric Institute, Department of Geriatric Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaInflammatory cytokines have crucial roles in the pathogenesis of tuberculosis (TB), and interleukin (IL)-27 and IL-35 have a pro-inflammatory and anti-inflammatory effect on many diseases, including infectious diseases. Therefore, we evaluated the relationship between IL-27 and IL-35 gene polymorphism, expression levels, and pulmonary TB (PTB) susceptibility. Nine single-nucleotide polymorphisms (SNPs) in the IL-27 gene (rs181206, rs153109, and rs17855750) and the IL-35 gene (rs4740, rs428253, rs9807813, rs2243123, rs2243135, and rs568408) were genotyped by the SNPscan technique in 497 patients with PTB and 501 controls. There was no significant difference regarding the genotype and allele frequencies of the above SNPs in the IL-27 and IL-35 genes between patients with PTB and controls. Haplotype analysis showed that the frequency of the GAC haplotype in the IL-35 gene was significantly decreased in patients with PTB when compared to controls (p = 0.036). Stratified analysis suggested that the frequency of the IL-27 rs17855750 GG genotype was significantly increased in patients with PTB with fever. Moreover, the lower frequency of the IL-35 rs568408 GA genotype was associated with drug-induced liver injury in patients with PTB. The IL-35 rs428253 GC genotype, as well as the rs4740 AA genotype and A allele, showed significant relationships with hypoproteinemia in patients with PTB. When compared with controls, the IL-27 level was significantly increased in patients with PTB. Taken together, IL-35 gene variation might contribute to a protective role on the susceptibility to PTB, and IL-27 and IL-35 gene polymorphisms were associated with several clinical manifestations of patients with PTB.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1267624/fullpulmonary tuberculosissingle-nucleotide polymorphismsIL-27IL-35infectious diseases |
| spellingShingle | Lei Gao Lei Gao Yan-Jun Xiong Ya-Xue Liang Peng-Fei Huang Shuang Liu Yu Xiao Qian Huang Hua Wang Hui-Mei Wu The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis Frontiers in Immunology pulmonary tuberculosis single-nucleotide polymorphisms IL-27 IL-35 infectious diseases |
| title | The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| title_full | The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| title_fullStr | The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| title_full_unstemmed | The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| title_short | The effects of IL-27 and IL-35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| title_sort | effects of il 27 and il 35 gene variation and expression levels on the susceptibility and clinical manifestations of pulmonary tuberculosis |
| topic | pulmonary tuberculosis single-nucleotide polymorphisms IL-27 IL-35 infectious diseases |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1267624/full |
| work_keys_str_mv | AT leigao theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT leigao theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yanjunxiong theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yaxueliang theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT pengfeihuang theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT shuangliu theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yuxiao theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT qianhuang theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT huawang theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT huimeiwu theeffectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT leigao effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT leigao effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yanjunxiong effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yaxueliang effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT pengfeihuang effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT shuangliu effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT yuxiao effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT qianhuang effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT huawang effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis AT huimeiwu effectsofil27andil35genevariationandexpressionlevelsonthesusceptibilityandclinicalmanifestationsofpulmonarytuberculosis |