Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-25454-1 |
| _version_ | 1818742626735620096 |
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| author | Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub |
| author_facet | Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub |
| author_sort | Martin Steger |
| collection | DOAJ |
| description | Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibitors. |
| first_indexed | 2024-12-18T02:15:31Z |
| format | Article |
| id | doaj.art-284693f5e9d14df9b7b8bbbd91efb49e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T02:15:31Z |
| publishDate | 2021-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-284693f5e9d14df9b7b8bbbd91efb49e2022-12-21T21:24:22ZengNature PortfolioNature Communications2041-17232021-09-0112111310.1038/s41467-021-25454-1Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scaleMartin Steger0Vadim Demichev1Mattias Backman2Uli Ohmayer3Phillip Ihmor4Stefan Müller5Markus Ralser6Henrik Daub7Evotec München GmbH, Am Klopferspitz 19aCharité – Universitätsmedizin Berlin, Department of BiochemistryEvotec München GmbH, Am Klopferspitz 19aEvotec München GmbH, Am Klopferspitz 19aEvotec München GmbH, Am Klopferspitz 19aEvotec München GmbH, Am Klopferspitz 19aCharité – Universitätsmedizin Berlin, Department of BiochemistryEvotec München GmbH, Am Klopferspitz 19aCombining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibitors.https://doi.org/10.1038/s41467-021-25454-1 |
| spellingShingle | Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale Nature Communications |
| title | Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale |
| title_full | Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale |
| title_fullStr | Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale |
| title_full_unstemmed | Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale |
| title_short | Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale |
| title_sort | time resolved in vivo ubiquitinome profiling by dia ms reveals usp7 targets on a proteome wide scale |
| url | https://doi.org/10.1038/s41467-021-25454-1 |
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