Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibit...
Main Authors: | Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-09-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-021-25454-1 |
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