HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas
<p><strong>.</strong> High performance liquid chromatography (HPLC) with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: <em&g...
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| Format: | Article |
| Language: | English |
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AcademicPres
2013-11-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| Subjects: | |
| Online Access: | http://journals.usamvcluj.ro/index.php/fst/article/view/9247 |
| _version_ | 1818259236686135296 |
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| author | Raluca Maria Pop Florina Csernatoni Floricuta Ranga Florinela Fetea Carmen Socaciu |
| author_facet | Raluca Maria Pop Florina Csernatoni Floricuta Ranga Florinela Fetea Carmen Socaciu |
| author_sort | Raluca Maria Pop |
| collection | DOAJ |
| description | <p><strong>.</strong> High performance liquid chromatography (HPLC) with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: <em>Taraxacum officinalis</em> (1), <em>Cynara scolimus</em> (2), <em>Silybum marianum</em> (3), <em>Hypericum perforatum</em> (4), <em>Chelidonium majus</em> (5), <em>Lycopodium clavatum</em> (6) and <em>Hippophae rhamnoides</em> (7) leaves and fruits. Two products (A and B) were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol) were analyzed using HPLC-UV for their phenolics’ fingerprint and composition.</p> <p>The qualitative (untargeted analysis) and quantitative (targeted analysis) results were further compared using Principal Component Analysis (PCA) in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were <em>trans</em>-cinnamic, chlorogenic, caffeic and <em>p-</em>coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids.</p> <p>In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.</p> |
| first_indexed | 2024-12-12T18:12:14Z |
| format | Article |
| id | doaj.art-2846a21747c84298a5a0b8b6cf82fc18 |
| institution | Directory Open Access Journal |
| issn | 2344-2344 2344-5300 |
| language | English |
| last_indexed | 2024-12-12T18:12:14Z |
| publishDate | 2013-11-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| spelling | doaj.art-2846a21747c84298a5a0b8b6cf82fc182022-12-22T00:16:22ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002013-11-017029910710.15835/buasvmcn-fst:92478115HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement FormulasRaluca Maria Pop0Florina Csernatoni1Floricuta Ranga2Florinela Fetea3Carmen Socaciu4UMF Cluj-NapocaUMF Cluj-NapocaUMF Cluj-NapocaUMF Cluj-NapocaUMF Cluj-Napoca<p><strong>.</strong> High performance liquid chromatography (HPLC) with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: <em>Taraxacum officinalis</em> (1), <em>Cynara scolimus</em> (2), <em>Silybum marianum</em> (3), <em>Hypericum perforatum</em> (4), <em>Chelidonium majus</em> (5), <em>Lycopodium clavatum</em> (6) and <em>Hippophae rhamnoides</em> (7) leaves and fruits. Two products (A and B) were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol) were analyzed using HPLC-UV for their phenolics’ fingerprint and composition.</p> <p>The qualitative (untargeted analysis) and quantitative (targeted analysis) results were further compared using Principal Component Analysis (PCA) in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were <em>trans</em>-cinnamic, chlorogenic, caffeic and <em>p-</em>coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids.</p> <p>In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.</p>http://journals.usamvcluj.ro/index.php/fst/article/view/9247medicinal plants, herbal supplements, HPLC fingerprint, Principal Component Analysis |
| spellingShingle | Raluca Maria Pop Florina Csernatoni Floricuta Ranga Florinela Fetea Carmen Socaciu HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology medicinal plants, herbal supplements, HPLC fingerprint, Principal Component Analysis |
| title | HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas |
| title_full | HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas |
| title_fullStr | HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas |
| title_full_unstemmed | HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas |
| title_short | HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas |
| title_sort | hplc uv analysis coupled with chemometry to identify phenolic biomarkers from medicinal plants used as ingredients in two food supplement formulas |
| topic | medicinal plants, herbal supplements, HPLC fingerprint, Principal Component Analysis |
| url | http://journals.usamvcluj.ro/index.php/fst/article/view/9247 |
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