Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm
Objectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)2) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus faecalis (EF) from the root canal system. Materials and Meth...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-06-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/2033 |
| _version_ | 1819182633065644032 |
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| author | Sholeh Ghabraei Behnam Bolhari Mohammad Marvi Sabbagh Mahsa Sobhi Afshar |
| author_facet | Sholeh Ghabraei Behnam Bolhari Mohammad Marvi Sabbagh Mahsa Sobhi Afshar |
| author_sort | Sholeh Ghabraei |
| collection | DOAJ |
| description | Objectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)2) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus faecalis (EF) from the root canal system.
Materials and Methods: Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)2 mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs).
Results: The paste of Ca(OH)2 mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days.
Conclusions: It seems that Ca(OH)2 mixed with 2% CHX is more potent than the TAP against EF biofilm. |
| first_indexed | 2024-12-22T22:49:13Z |
| format | Article |
| id | doaj.art-284979f764a8419b956fd075d95d9000 |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-12-22T22:49:13Z |
| publishDate | 2018-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-284979f764a8419b956fd075d95d90002022-12-21T18:10:00ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2018-06-01153966Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis BiofilmSholeh Ghabraei0Behnam Bolhari1Mohammad Marvi Sabbagh2Mahsa Sobhi Afshar3Assistant Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranDentist, Private Practice, Tehran, IranPostgraduate Student, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranObjectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)2) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus faecalis (EF) from the root canal system. Materials and Methods: Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)2 mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs). Results: The paste of Ca(OH)2 mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days. Conclusions: It seems that Ca(OH)2 mixed with 2% CHX is more potent than the TAP against EF biofilm.https://jdt.tums.ac.ir/index.php/jdt/article/view/2033DoxycyclineMetronidazoleCiprofloxacinCalcium HydroxideChlorhexidineEnterococcus Faecalis |
| spellingShingle | Sholeh Ghabraei Behnam Bolhari Mohammad Marvi Sabbagh Mahsa Sobhi Afshar Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm Frontiers in Dentistry Doxycycline Metronidazole Ciprofloxacin Calcium Hydroxide Chlorhexidine Enterococcus Faecalis |
| title | Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm |
| title_full | Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm |
| title_fullStr | Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm |
| title_full_unstemmed | Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm |
| title_short | Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm |
| title_sort | comparison of antimicrobial effects of triple antibiotic paste and calcium hydroxide mixed with 2 chlorhexidine as intracanal medicaments against enterococcus faecalis biofilm |
| topic | Doxycycline Metronidazole Ciprofloxacin Calcium Hydroxide Chlorhexidine Enterococcus Faecalis |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/2033 |
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