Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention
ObjectiveTo establish an ischemia-reperfusion model in cynomolgus macaques and to analyse the effects of edaravone intervention.MethodsA total of fifteen adult male cynomolgus macaques were randomly divided into three groups: sham operation (Sham group, n=3), ischemia-reperfusion model (Model group,...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2023-04-01
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| Series: | Shiyan dongwu yu bijiao yixue |
| Subjects: | |
| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.165 |
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| author | PAN Mengxian HUANG Xiaojiao HUANG Zhongli SHEN Guo ZHANG Pengfei ZENG Yong LI Wenfeng ZHOU Huabo WEI Zhumei |
| author_facet | PAN Mengxian HUANG Xiaojiao HUANG Zhongli SHEN Guo ZHANG Pengfei ZENG Yong LI Wenfeng ZHOU Huabo WEI Zhumei |
| author_sort | PAN Mengxian |
| collection | DOAJ |
| description | ObjectiveTo establish an ischemia-reperfusion model in cynomolgus macaques and to analyse the effects of edaravone intervention.MethodsA total of fifteen adult male cynomolgus macaques were randomly divided into three groups: sham operation (Sham group, n=3), ischemia-reperfusion model (Model group, n=6) and edaravone treatment (Edaravone group, n=6). Ischemic-reperfusion model of cynomolgus macaques was established by clamping the M1 branch of the left cerebral artery for 1 h. After 2 h of reperfusion, the animals in Edaravone group were injected with 0.5 mg/kg edaravone intravenously for intervention treatment, while the animals in Sham and Model groups were injected with an equal volume of normal saline intravenously, twice a day, from the 2nd to 7th day. The behavioral video recordings, clinical observations and neurological deficit scores of cynomolgus macaques were obtained, and brain edema volume and cerebral ischemia volume were statistically analyzed.ResultsCompared with the Sham group, the animals in Model group showed typical symptoms of ischemic stroke, with a significant increase in the neurological deficit score， the volumes of edema and infarct of brain tissue (all P＜0.01). Compared with Model group, the neurological deficit score, the volumes of edema and infarct of brain tissue were significantly reduced in Edaravone group (all P＜0.05).ConclusionAn animal model of ischemia-reperfusion in cynomolgus macaques was successfully established, and edaravone was confirmed to alleviate the damage caused by ischemia-reperfusion. |
| first_indexed | 2024-03-13T10:53:03Z |
| format | Article |
| id | doaj.art-28499584c529420ea33ec84cc1e89eff |
| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-03-13T10:53:03Z |
| publishDate | 2023-04-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-28499584c529420ea33ec84cc1e89eff2023-05-17T06:57:29ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172023-04-0143213614410.12300/j.issn.1674-5817.2022.1651674-5817（2023）02-0136-09Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone InterventionPAN Mengxian0HUANG Xiaojiao1HUANG Zhongli2SHEN Guo3ZHANG Pengfei4ZENG Yong5LI Wenfeng6ZHOU Huabo7WEI Zhumei8Guangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaGuangxi Thinxon Biomedical Technology Co., LTD., Nanning 530000, ChinaNanning Huabo Pet Hospital, Nanning 530000, ChinaGuangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, ChinaObjectiveTo establish an ischemia-reperfusion model in cynomolgus macaques and to analyse the effects of edaravone intervention.MethodsA total of fifteen adult male cynomolgus macaques were randomly divided into three groups: sham operation (Sham group, n=3), ischemia-reperfusion model (Model group, n=6) and edaravone treatment (Edaravone group, n=6). Ischemic-reperfusion model of cynomolgus macaques was established by clamping the M1 branch of the left cerebral artery for 1 h. After 2 h of reperfusion, the animals in Edaravone group were injected with 0.5 mg/kg edaravone intravenously for intervention treatment, while the animals in Sham and Model groups were injected with an equal volume of normal saline intravenously, twice a day, from the 2nd to 7th day. The behavioral video recordings, clinical observations and neurological deficit scores of cynomolgus macaques were obtained, and brain edema volume and cerebral ischemia volume were statistically analyzed.ResultsCompared with the Sham group, the animals in Model group showed typical symptoms of ischemic stroke, with a significant increase in the neurological deficit score， the volumes of edema and infarct of brain tissue (all P＜0.01). Compared with Model group, the neurological deficit score, the volumes of edema and infarct of brain tissue were significantly reduced in Edaravone group (all P＜0.05).ConclusionAn animal model of ischemia-reperfusion in cynomolgus macaques was successfully established, and edaravone was confirmed to alleviate the damage caused by ischemia-reperfusion.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.165cynomolgus macaquesischemic strokeischemia-reperfusionedaravone |
| spellingShingle | PAN Mengxian HUANG Xiaojiao HUANG Zhongli SHEN Guo ZHANG Pengfei ZENG Yong LI Wenfeng ZHOU Huabo WEI Zhumei Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention Shiyan dongwu yu bijiao yixue cynomolgus macaques ischemic stroke ischemia-reperfusion edaravone |
| title | Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention |
| title_full | Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention |
| title_fullStr | Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention |
| title_full_unstemmed | Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention |
| title_short | Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention |
| title_sort | establishment of ischemia reperfusion model in cynomolgus macaques and effects of edaravone intervention |
| topic | cynomolgus macaques ischemic stroke ischemia-reperfusion edaravone |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.165 |
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