Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage
It has previously demonstrated that adenine (6-amino-6H-purine) exhibited a vasoactive effect in contracted aorta. The adenine receptor (AdeR) has recently been identified as a G protein coupled purinergic receptor in diverse organs. It has been suggested that adenine acts as a signaling molecule th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616303553 |
| _version_ | 1819292699014987776 |
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| author | Toshihiko Fukuda Kaustav Majumder Hua Zhang Toshiro Matsui Yoshinori Mine |
| author_facet | Toshihiko Fukuda Kaustav Majumder Hua Zhang Toshiro Matsui Yoshinori Mine |
| author_sort | Toshihiko Fukuda |
| collection | DOAJ |
| description | It has previously demonstrated that adenine (6-amino-6H-purine) exhibited a vasoactive effect in contracted aorta. The adenine receptor (AdeR) has recently been identified as a G protein coupled purinergic receptor in diverse organs. It has been suggested that adenine acts as a signaling molecule through the activation of AdeR. The present study investigated the effect of adenine on endotoxin-stimulated mouse macrophages (RAW 264.7 cells) and focused on the AdeR signaling pathway. RAW 264.7 cells were treated with adenine and stimulated with lipopolysaccharide (LPS, Toll-like receptor 4 agonist) from Escherichia coli. Adenine inhibited tumor necrosis factor (TNF)-α secretion in RAW 264.7 cells. The inhibition by adenine was significantly attenuated in the presence of either adenyl cyclase (AC) or protein kinase A (PKA) inhibitors. Furthermore, adenine restored AdeR gene expression. These results firstly demonstrated that adenine acts as an anti-endotoxin compounds through a AdeR/AC/PKA signaling cascade. |
| first_indexed | 2024-12-24T03:58:41Z |
| format | Article |
| id | doaj.art-284ba9f9bbdc4dbdb59a0cca7ce84512 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T03:58:41Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-284ba9f9bbdc4dbdb59a0cca7ce845122022-12-21T17:16:22ZengElsevierJournal of Functional Foods1756-46462017-01-0128235239Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophageToshihiko Fukuda0Kaustav Majumder1Hua Zhang2Toshiro Matsui3Yoshinori Mine4Department of Food Science, University of Guelph, Guelph, Ontario N1G2W1, Canada; Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanDepartment of Food Science, University of Guelph, Guelph, Ontario N1G2W1, CanadaDepartment of Food Science, University of Guelph, Guelph, Ontario N1G2W1, CanadaDivision of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanDepartment of Food Science, University of Guelph, Guelph, Ontario N1G2W1, Canada; Corresponding author.It has previously demonstrated that adenine (6-amino-6H-purine) exhibited a vasoactive effect in contracted aorta. The adenine receptor (AdeR) has recently been identified as a G protein coupled purinergic receptor in diverse organs. It has been suggested that adenine acts as a signaling molecule through the activation of AdeR. The present study investigated the effect of adenine on endotoxin-stimulated mouse macrophages (RAW 264.7 cells) and focused on the AdeR signaling pathway. RAW 264.7 cells were treated with adenine and stimulated with lipopolysaccharide (LPS, Toll-like receptor 4 agonist) from Escherichia coli. Adenine inhibited tumor necrosis factor (TNF)-α secretion in RAW 264.7 cells. The inhibition by adenine was significantly attenuated in the presence of either adenyl cyclase (AC) or protein kinase A (PKA) inhibitors. Furthermore, adenine restored AdeR gene expression. These results firstly demonstrated that adenine acts as an anti-endotoxin compounds through a AdeR/AC/PKA signaling cascade.http://www.sciencedirect.com/science/article/pii/S1756464616303553AdenineAdenine receptorMacrophageLPSAnti-inflammation |
| spellingShingle | Toshihiko Fukuda Kaustav Majumder Hua Zhang Toshiro Matsui Yoshinori Mine Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage Journal of Functional Foods Adenine Adenine receptor Macrophage LPS Anti-inflammation |
| title | Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| title_full | Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| title_fullStr | Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| title_full_unstemmed | Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| title_short | Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| title_sort | adenine has an anti inflammatory effect through the activation of adenine receptor signaling in mouse macrophage |
| topic | Adenine Adenine receptor Macrophage LPS Anti-inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616303553 |
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