Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain
Summary: Astrocytes interact with not only synapses but also brain blood vessels through perivascular astrocyte endfeet (PV-AEF) to form the neurovascular unit (NVU). However, PV-AEF components have not been fully identified. Here, we biochemically isolated blood vessels from mouse brain homogenates...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223020874 |
| _version_ | 1797647803794587648 |
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| author | Takeshi Kameyama Muneaki Miyata Hajime Shiotani Jun Adachi Soichiro Kakuta Yasuo Uchiyama Kiyohito Mizutani Yoshimi Takai |
| author_facet | Takeshi Kameyama Muneaki Miyata Hajime Shiotani Jun Adachi Soichiro Kakuta Yasuo Uchiyama Kiyohito Mizutani Yoshimi Takai |
| author_sort | Takeshi Kameyama |
| collection | DOAJ |
| description | Summary: Astrocytes interact with not only synapses but also brain blood vessels through perivascular astrocyte endfeet (PV-AEF) to form the neurovascular unit (NVU). However, PV-AEF components have not been fully identified. Here, we biochemically isolated blood vessels from mouse brain homogenates and purified PV-AEF. The purified PV-AEF were observed in different sizes, similar to PV-AEF on brain blood vessels. Mass spectrometry analysis identified 9,762 proteins in the purified PV-AEF, including cell adhesion molecules, nectin-2δ, Kirrel2, and podoplanin. Immunofluorescence microscopic analysis revealed that nectin-2δ and podoplanin were concentrated mainly in arteries/arterioles and veins/venules of the mouse brain, whereas Kirrel2 was mainly in arteries/arterioles. Nectin-2α/δ, Kirrel2, and podoplanin were preferentially observed in large sizes of the purified PV-AEF. Furthermore, Kirrel2 potentially has cell adhesion activity of cultured astrocytes. Collectively, these results indicate that PV-AEF have heterogeneity in sizes and molecular components, implying different roles of PV-AEF in NVU function depending on vascular regions. |
| first_indexed | 2024-03-11T15:21:47Z |
| format | Article |
| id | doaj.art-284f87b094cb43ccba6d8301bf19a4f1 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:21:47Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-284f87b094cb43ccba6d8301bf19a4f12023-10-28T05:09:17ZengElsevieriScience2589-00422023-10-012610108010Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brainTakeshi Kameyama0Muneaki Miyata1Hajime Shiotani2Jun Adachi3Soichiro Kakuta4Yasuo Uchiyama5Kiyohito Mizutani6Yoshimi Takai7Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan; Department of Immunology and Parasitology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, JapanDivision of Pathogenetic Signaling, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan; Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, JapanDivision of Pathogenetic Signaling, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan; Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, JapanLaboratory of Proteomics for Drug Discovery, Center for Drug Design Research, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan; Laboratory of Clinical and Analytical Chemistry, Center for Drug Design Research, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, JapanLaboratory of Morphology and Image Analysis, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan; Department of Cellular Molecular Neuropathology, Juntendo University Graduate School of Medicine, Tokyo 113-8421, JapanDepartment of Cellular Molecular Neuropathology, Juntendo University Graduate School of Medicine, Tokyo 113-8421, JapanDivision of Pathogenetic Signaling, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan; Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, Japan; Corresponding authorDivision of Pathogenetic Signaling, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan; Corresponding authorSummary: Astrocytes interact with not only synapses but also brain blood vessels through perivascular astrocyte endfeet (PV-AEF) to form the neurovascular unit (NVU). However, PV-AEF components have not been fully identified. Here, we biochemically isolated blood vessels from mouse brain homogenates and purified PV-AEF. The purified PV-AEF were observed in different sizes, similar to PV-AEF on brain blood vessels. Mass spectrometry analysis identified 9,762 proteins in the purified PV-AEF, including cell adhesion molecules, nectin-2δ, Kirrel2, and podoplanin. Immunofluorescence microscopic analysis revealed that nectin-2δ and podoplanin were concentrated mainly in arteries/arterioles and veins/venules of the mouse brain, whereas Kirrel2 was mainly in arteries/arterioles. Nectin-2α/δ, Kirrel2, and podoplanin were preferentially observed in large sizes of the purified PV-AEF. Furthermore, Kirrel2 potentially has cell adhesion activity of cultured astrocytes. Collectively, these results indicate that PV-AEF have heterogeneity in sizes and molecular components, implying different roles of PV-AEF in NVU function depending on vascular regions.http://www.sciencedirect.com/science/article/pii/S2589004223020874Vascular anatomyImmunologyMolecular neuroscience |
| spellingShingle | Takeshi Kameyama Muneaki Miyata Hajime Shiotani Jun Adachi Soichiro Kakuta Yasuo Uchiyama Kiyohito Mizutani Yoshimi Takai Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain iScience Vascular anatomy Immunology Molecular neuroscience |
| title | Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| title_full | Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| title_fullStr | Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| title_full_unstemmed | Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| title_short | Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| title_sort | heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain |
| topic | Vascular anatomy Immunology Molecular neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223020874 |
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